Evaluation of angiogenesis in diabetic lower limb wound healing using a natural medicine: A quantitative approach

Barui, Ananya; Banerjee, P.; Chaudhary, Amrita; Conjeti, Sailesh; Mondal, B.; Dey, Susmita; Chatterjee, Jyotirmoy

doi:10.1016/j.wndm.2014.09.003

Cited by 6 publications

(14 citation statements)

References 33 publications

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“…The surplus reactive oxygen species (ROS) generation and oxidative stress contributed by high glucose ambience which causes impairment of HIF-1α regulation in diabetes. 31 Several studies were reported about the relevance of various hydroxylase inhibitors viz. DMOG, DFX, etc.…”

Section: Resultsmentioning

confidence: 99%

Wound healing efficacy of Jamun honey in diabetic mice model through reepithelialization, collagen deposition and angiogenesis

Chaudhary

Bag

Banerjee³

et al. 2020

Journal of Traditional and Complementary Medicine

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Diabetic patients are frequently afflicted with impaired wound healing where linear progression of molecular and cellular events compromised. Despite of meaningful progress in diabetic treatment, management of diabetic chronic wounds is still challenging. Jamun ( Syzygium cumini ) honey may be a promising candidate for diabetic wound healing and need to explore in detail. So present study was designed to evaluate the efficacy of Jamun honey (JH) for diabetic wound healing in in vitro wound (primary fibroblasts) model and in in vivo of diabetic mice (Streptozotocin induced) model. The fibroblast cell model was studied for migratory behaviour and myofibrolasts infiltration under honey interventions via scratch/migration assay, immuno-cytochemistry and western blot. We applied FDA approved Manuka honey (MH) as positive control and JH as test honey to evaluate wound re-epithelialization, sub-epithelial connective tissue modification and angiogenesis via histo-pathological and immuno-histochemical analysis. JH (0.1% v/v) dilution has notably improved wound closure, migration with concomitant α-SMA expressions in vitro . Topical application of JH in diabetic mice model showed significant (*p ≤ 0.05) wound closure, reepithelialization, collagen deposition (I/III) and balanced the myofibroblasts formation. It also modulated vital angiogenic markers ( viz HIF-1α, VEGF, VEGF R–II) significantly (*p ≤ 0.05). All these observations depicted that JH promotes sequential stages of wound healing in diabetic mice model. The results of the present study established Jamun honey as good as Manuka honey considering wound closure, re-epithelialization, collagen deposition and pro-angiogenic potential.

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Section: Resultsmentioning

confidence: 99%

Wound healing efficacy of Jamun honey in diabetic mice model through reepithelialization, collagen deposition and angiogenesis

Chaudhary

Bag

Banerjee³

et al. 2020

Journal of Traditional and Complementary Medicine

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“…Essa composição cria um gradiente osmótico, fornecendo um ambiente com umidade equilibrada (Mohamed et al, 2014). Um ensaio clínico de Barui et al (2014) com seis pacientes analisou as características química e físicas do mel, demonstrando que uma grande quantidade de açúcares, juntamente com uma quantidade mínima de água livre, pode facilitar a retenção ideal de umidade no leito da ferida. Research, Society and Development, v. 9, n. 7, e956974663, 2020 (CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v9i7.4663…”

Section: Resultsunclassified

Benefícios do uso do mel no tratamento do pé diabético: Scoping Review

Cruz

Monteiro

Silva

et al. 2020

RSD

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Diabetic foot can be understood as the destruction of soft tissues, and as infections or ulcerations associated with neurological and vascular changes in the lower limbs of patients with Diabetes Mellitus (DM). It is also one of the most severe, costly, and devastating complications of DM, and foot ulcers are precursors of lower limb amputations in 85% of the cases. Thus, honey emerges as an alternative treatment which is economical, presents a low risk of bacterial resistance, and is widely available, indispensable factors for the continuity of treatment by the patients. This article is a scoping review with the objective of describing the evidence to elucidate the benefits of honey in the treatment of diabetic foot. The research includes primary studies, reviews, meta-analyses and/or meta-syntheses, books and guidelines, theses, and dissertations, published in indexed sources or in the gray literature published in the following databases: Scielo, LILACS, Pubmed, The Cochrane Library, Web of Science, SCOPUS, CINAHL, and Academic Search Premier, CAPS, the USP Digital Library of Theses and Dissertations, and the USP Integrated Search Portal. In the literature reviewed, 33 different benefits have been found which are described for the use of honey. Among these benefits, the higher healing rate and anti-microbial, anti-inflammatory, and debriding properties stand out, among others. Therefore, the analyzed studies showed a wide variety of benefits, which reflects the complexity of the action and the usefulness of this product.

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“…One possible reason behind mitigating the consequences of hypoxia and its related gene Hif 1α is newly formed free oxygen, which is generated by decomposition of H 2 O 2 into water and oxygen. The degradation of H 2 O 2 is catalyzed by pollen source derived enzyme catalase and this enzyme activity reported to increase with honey dilution . Furthermore, this process also produces superoxide anion radical which is again reduced by methyl syringate present in honey, this in turn induces wound healing process by decreasing oxidative stress both extracellularly and intracellularly .…”

Section: Discussionmentioning

confidence: 99%

“…The degradation of H 2 O 2 is catalyzed by pollen source derived enzyme catalase 32 and this enzyme activity reported to increase with honey dilution. 10 Furthermore, this process also produces superoxide anion radical which is again reduced by methyl syringate present in honey, this in turn induces wound healing process by decreasing oxidative stress both extracellularly and intracellularly. 39 Hence, all these findings support improved in vitro wound healing in hypoxic under 0.1% honey dilution.…”

Section: Discussionmentioning

confidence: 99%

“…9 Presently, researchers are trying to search complementary, alternative, and cost efficient medicine to persuade appropriate conditions for natural healing process. In this regard, healing efficacy of honey against tropical infections and wounds mentioned by some researchers in last few years, 10 but observations of honey dilutions are scanty. Being hygroscopic in nature, honey gets diluted by absorbing biofluids along with systemic assimilation of relevant bioactive components.…”

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confidence: 99%

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Honey dilution impact on in vitro wound healing: Normoxic and hypoxic condition

Chaudhary

Bag

Barui

et al. 2015

Wound Repair Regeneration

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Honey is known as a popular healing agent against tropical infections and wounds. However, the effects of honey dilutions on keratinocyte (HaCaT) wound healing under hypoxic condition is still not explored. In this study, we examined whether honey dilution have wound healing potential under hypoxic stress. The antioxidant potential and healing efficacy of honey dilution on in vitro wound of human epidermal keratinocyte (HaCaT cells) under hypoxia (3% O2 ), and normoxia is explored by nitro blue tetrazolium assay. The cell survival % quantified by MTT assay to select four honey dilutions like 10, 1, 0.1, and 0.01 v/v% and the changes in cellular function was observed microscopically. Further, the cell proliferation, migration, cell-cell adhesion, and relevant gene expression were studied by flow cytometry, migration/scratch assay, immunocytochemistry, and reverse transcription-polymerase chain reaction, respectively. The expression pattern of cardinal molecular features viz. E-cadherin, cytoskeletal protein F-actin, p63, and hypoxia marker Hif 1α were examined. Honey dilution in 0.1% v/v combat wound healing limitations in vitro under normoxia and hypoxia (3%). Its wound healing potential was quantified by immunocytochemistry and real-time PCR for the associated molecular features that were responsible for cell proliferation and migration. Our data showed that honey dilution can be effective in hypoxic wound healing. Additionally, it reduced superoxide generation and supplied favorable bioambience for cell proliferation, migration, and differentiation during hypoxic wound healing. These findings may reveal the importance of honey as an alternative and cost effective therapeutic natural product for wound healing in hypoxic condition.

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Evaluation of angiogenesis in diabetic lower limb wound healing using a natural medicine: A quantitative approach

Cited by 6 publications

References 33 publications

Wound healing efficacy of Jamun honey in diabetic mice model through reepithelialization, collagen deposition and angiogenesis

Wound healing efficacy of Jamun honey in diabetic mice model through reepithelialization, collagen deposition and angiogenesis

Benefícios do uso do mel no tratamento do pé diabético: Scoping Review

Honey dilution impact on in vitro wound healing: Normoxic and hypoxic condition

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