Evaluation of an Ionic Calcium Fiber Supplement and Its Impact on Bone Health Preservation in a Dietary Calcium Deficiency Mice Model

Abstract

ionic calcium can help in the prevention of the process of osseous decalcification. This study aimed to evaluate the physicochemical properties and toxic effects of ionic calcium-fiber supplement (ICa+) and its impact on bone health preservation in mice C57/BL6 fed a calcium-deficient diet. Physicochemical properties include FTIR, apparent calcium solubility estimated by the calcium ratio obtained by ionization chromatography and atomic absorption. In vitro genotoxicity and cytotoxicity of the ICa+ were assessed. Twenty-five 7-week-old C57/BL6 mice were fed calcium-free diet (CFD) or CFD plus CaCO3 (1.33 mg Ca) or CFD plus ICa+ (1.33–6.66 mg Ca) for six weeks. After that, bone mass and microstructure parameters were assessed. Histological staining was performed to determine calcium deposits. ICa+ (100%) exhibited an apparent calcium solubility higher than CaCO3 (12.3%). ICa+ showed no cytotoxic and genotoxic in vitro activities. Histomorphometry analysis showed that the ICa+ treated group displayed a higher trabecular number than the trabecular space. Also, the ratio BV/TV was increased compared with all treatments. Ionic calcium-fiber supplementation prevents bone deterioration compared to mice fed a calcium-deficient diet.

Anti-osteoporotic Drug Utilization Rates for Secondary Prevention Among Patients with Osteoporotic Fractures

“…Moreover, some researchers suggest that ionic calcium supplements such as antiorbital ionic calcium may provide greater benefit to osteoporosis patients than conventional calcium intake. 22,23 Furthermore, since osteoporosis patients have a high prevalence of vitamin D deficiency, vitamin D supplement becomes a necessity, even in patients receiving AOD. 19 The course of osteoporosis is mostly silent until it is complicated by fractures.…”

Preparation of synbiotic milk powder and its effect on calcium absorption and the bone microstructure in calcium deficient mice

“…The increase in cancellous bone volume fraction is primarily due to an increase in the number of bone trabeculae. 60 Fig. 8C shows that the number of bone trabeculae in the female HD group was significantly higher ( p < 0.05) than that in the female LC group.…”