Evaluation of an intensifier for 14C and 3H autoradiography

Meeks, Marion J.; Kessler, Wayne V.; Born, Gordon S.; Landolt, Robert R.

doi:10.1016/0020-708x(75)90107-6

Cited by 4 publications

(1 citation statement)

References 7 publications

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“…Despite various proposed processes, physical development after gold latensification (Wisse & Tates, 1968) or short preexposure of the emulsion with photons (Hillson & Sutherns, 1967), the maximum yield, at the present time, is of the order of one silver grain for five emitted electrons (Vrensen, 1970). For the detection of low radioactivities in chromatographic gels by X-ray films, increased efficiency was obtained by impregnating the gel with scintillating compounds (Wilson, 1958;Liithi & Waser, 1965;Randerath, 1970;Bonner & Laskey, 1974;Meeks et al, 1975). The only technical limitations are the necessity of using tritium as the labelling isotope and the low temperature o f expxure (193 K).…”

Section: Introductionmentioning

confidence: 99%

Electron microscope fluoro‐autoradiography: improvement of efficiency

Buchel¹,

Delain²,

Bouteille³

1978

Journal of Microscopy

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The fluorographic process for enhancement of the autoradiographic detection of beta-ray emitted by tritium has been successfully adapted to electron microscope autoradiography. An original scintillator plastic film helped to increase the detection yield of electrons up to 80% without alteration of the morphological aspect of the autoradiograms, and without increasing background fog or causing apparent loss of resolution.

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Section: Introductionmentioning

confidence: 99%

Electron microscope fluoro‐autoradiography: improvement of efficiency

Buchel¹,

Delain²,

Bouteille³

1978

Journal of Microscopy

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Quantitative investigation of scintillator intensification for light and electron microscope radioautography

Kopriwa

1980

Histochemistry

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Several reports have indicated that scintillators enhance the intensity of radioautographic reactions. If this were the case, the use of scintillators would shorten the exposure time of radioautographs and the production of radioautographs would be accelerated. It was, therefore, decided to examine the effect of scintillators on the intensity of radioautographs in a quantitative manner over uniformly labeled test specimens. Reaction intensities with or without scintillators were compared using Kodak NTB2 emulsion for light microscopy and Ilford L4 and Sakura NR-H2 emulsion for electron microscopy. In addition, the background fog was quantitatively evaluated. No increase in the intensity of the radioautographs was observed under the following conditions: pretreatment of tissue sections with scintillator solutions before emulsion coating and exposure; treatment with scintillator solutions after emulsion coating but before exposure; treatment with scintillator solutions during exposure; contact with a plastic scintillator film throughout exposure. Heightened reaction intensity was obtained only when scintillators were applied during the histological processing of tissues. The impregnation of tissue blocks with PPO (2,5-Diphenyloxazole) and POPOP (1,4-bis-2-(5-phenyloxazolyl)-benzene) during dehydration, infiltration and Epon embedding produced a 20% increase of reaction intensity in light microscope radioautographs using NTB2 emulsion and a 75% increase in electron microscope radioautographs using L4 emulsion.

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