Evaluation of an indirect ELISA using a tachyzoite surface antigen SAG1 for diagnosis of Toxoplasma gondii infection in cats

Hosseininejad, M.

doi:10.1016/j.exppara.2012.09.009

Cited by 9 publications

(4 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…122.5938-9). Details on the experimental infections conducted in Iran and the serological results of the kittens were published elsewhere [41]. Sera from cats in Germany and Switzerland were collected for routine veterinary diagnostic purposes and not for the purpose of research; consequently no ethical approval was needed.…”

Section: Methodsmentioning

confidence: 99%

“…Sera from cats experimentally infected with tachyzoites at the Veterinary Faculty of Shahrekord University, Iran: Six 45±5-day-old clinically healthy kittens of both genders were infected intraperitoneally with 10 4 T. gondii RH tachyzoites in sterile PBS as described previously [41]. In five of six kittens infected with RH tachyzoites, serum samples were collected on days 18 and 26 post infections.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Serotyping of Toxoplasma gondii in Cats (Felis domesticus) Reveals Predominance of Type II Infections in Germany

et al. 2013

View full text Add to dashboard Cite

BackgroundCats are definitive hosts of Toxoplasma gondii and play an essential role in the epidemiology of this parasite. The study aims at clarifying whether cats are able to develop specific antibodies against different clonal types of T. gondii and to determine by serotyping the T. gondii clonal types prevailing in cats as intermediate hosts in Germany.MethodologyTo establish a peptide-microarray serotyping test, we identified 24 suitable peptides using serological T. gondii positive (n=21) and negative cat sera (n=52). To determine the clonal type-specific antibody response of cats in Germany, 86 field sera from T. gondii seropositive naturally infected cats were tested. In addition, we analyzed the antibody response in cats experimentally infected with non-canonical T. gondii types (n=7).FindingsPositive cat reference sera reacted predominantly with peptides harbouring amino acid sequences specific for the clonal T. gondii type the cats were infected with. When the array was applied to field sera from Germany, 98.8% (85/86) of naturally-infected cats recognized similar peptide patterns as T. gondii type II reference sera and showed the strongest reaction intensities with clonal type II-specific peptides. In addition, naturally infected cats recognized type II-specific peptides significantly more frequently than peptides of other type-specificities. Cats infected with non-canonical types showed the strongest reactivity with peptides presenting amino-acid sequences specific for both, type I and type III.ConclusionsCats are able to mount a clonal type-specific antibody response against T. gondii. Serotyping revealed for most seropositive field sera patterns resembling those observed after clonal type II-T. gondii infection. This finding is in accord with our previous results on the occurrence of T. gondii clonal types in oocysts shed by cats in Germany.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Serotyping of Toxoplasma gondii in Cats (Felis domesticus) Reveals Predominance of Type II Infections in Germany

et al. 2013

View full text Add to dashboard Cite

show abstract

“…By contrast, multiple serologic tests including indirect haemagglutination test (IHAT), modified agglutination test (MAT), latex agglutination test (LAT), indirect fluorescent antibody test (IFAT), and enzyme-linked immunosorbent assay (ELISA) are more optimized and practical. ELISA, in particular, is widely utilized for its convenience in antigen-specific antibody response detection across various laboratories [ 5 , 6 ]. However, test performance depends mostly on diagnostic antigens.…”

Section: Introductionmentioning

confidence: 99%

“…Consequently, numerous studies have reported the acquisition of false-positive results along with poor specificity and sensitivity for T. gondii for this method [ 7 ]. In the past decade, multiple recombinant antigens with serodiagnostic potential were identified, which includes dense granule proteins (GRA) [ 8 – 10 ], the surface antigens (SAG) [ 5 , 11 ], microneme proteins (MIC), cyst matrix antigen (MAG1), apical membrane antigen (AMA) [ 12 ], and the rhoptry proteins (ROP) [ 13 , 14 ]. In the aforementioned studies, recombinant protein antigens were tested using the sera acquired from T. gondii -infected humans and animals.…”

Section: Introductionmentioning

confidence: 99%

Detection of Toxoplasma gondii Infections using Virus-Like Particles Displaying T. gondii ROP4 Antigen

Kim¹,

Mao²,

Kang³

et al. 2021

Korean J Parasitol

View full text Add to dashboard Cite

Toxoplasma gondii ME49 infections are typically diagnosed by serological tests. However, serological diagnosis of RH strain-induced toxoplasmosis remains unknown. In order to develop seradiagnosis of above 2 kinds of infections, we generated recombinant virus-like particles (VLPs) displaying the T. gondii rhoptry protein 4 (ROP4) and evaluated their potential in T. gondii ME49 or RH strain infection diagnostics. Mice were orally infected with either the tachyzoites of T. gondii (RH) or cysts of T. gondii (ME49) at various dosages, and sera were collected at regular intervals. ELISA-based serological tests were performed to assess IgG, IgM, and IgA antibody responses against ROP4 VLP antigen and tissue lysate antigen (TLA). Compared to TLA, IgG, IgM, and IgA levels to ROP4 VLP antigen were significantly higher in the sera of T. gondii RH-infected mice 1 and 2 week post-infection (PI). T. gondii-specific IgG antibody was detected at 1, 2, 4, and 8 week PI in the T. gondii ME49-infected mice with infection dose-dependent manner. These results indicated that the ROP4 VLP antigen was highly sensitive antigens detecting T. gondii RH and ME49 antibodies at an early stage.

show abstract

Evaluation of recombinant antigens in combination and single formula for diagnosis of feline toxoplasmosis

Abdelbaset

Alhasan

Salman

et al. 2017

Experimental Parasitology

View full text Add to dashboard Cite

Cats are the only definitive hosts of Toxoplasma gondii and constitute an essential source of infection to all warm blooded animals and humans. Diagnosis of T. gondii infection in cats is fundamental for proper management and control of infection in humans and animals. In the current study, we have evaluated the diagnostic performance of tachyzoite lysate antigen (TLA) and different T. gondii recombinant antigens including surface antigen 2 (SAG2), dense granule proteins 2, 6, 7, 15 (GRA2, GRA6, GRA7, GRA15) and microneme 10 protein (MIC10) in immunoglobulin G enzyme linked-immunosorbent assay (IgG ELISA) using cat serum samples, with reference to latex agglutination test (LAT). Remarkably, TLA showed better performance than other recombinant antigens in IgG ELISAs as compared to LAT, with concordance and Kappa values of 94.27% and 0.93, respectively. Furthermore, to improve the reactivity of the recombinant antigens, we have developed IgG ELISAs using different combinations with these recombinant antigens. Strikingly, a combination of SAG2 and GRAs has relatively similar performance as TLA evidenced by concordance and Kappa values of 94.27% and 0.81, respectively. The developed ELISA with a combination of recombinant antigens can be used as a promising diagnostic tool for routine testing of T. gondii infection and mass screening in cats. The major advantages of this assay are the high sensitivity and specificity, lower cost, safer production and easiness of standardization in various laboratories worldwide.

show abstract

Evaluation of an indirect ELISA using a tachyzoite surface antigen SAG1 for diagnosis of Toxoplasma gondii infection in cats

Cited by 9 publications

References 24 publications

Serotyping of Toxoplasma gondii in Cats (Felis domesticus) Reveals Predominance of Type II Infections in Germany

Serotyping of Toxoplasma gondii in Cats (Felis domesticus) Reveals Predominance of Type II Infections in Germany

Detection of Toxoplasma gondii Infections using Virus-Like Particles Displaying T. gondii ROP4 Antigen

Evaluation of recombinant antigens in combination and single formula for diagnosis of feline toxoplasmosis

Contact Info

Product

Resources

About