Evaluation of an indirect ELISA using recombinant granule antigen GRA1, GRA7 and soluble antigens for serodiagnosis of Toxoplasma gondii infection in chickens

Sun, Xiaohong; Wang, Zedong; Li, Jiping; Wei, Feng; Liu, Quan

doi:10.1016/j.rvsc.2015.04.011

Cited by 25 publications

(25 citation statements)

References 26 publications

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“…Although there was no solid and direct comparison between these different types of ELISA tests, from literature it seems like SAG and GRA7 based indirect ELISAs are the most reliable. The sensitivity and specificity were claimed to be nearly 100% and above 96%, respectively (Lu et al, 2006 ; Sun et al, 2015 ).…”

Section: Development Of Diagnostic Approachesmentioning

confidence: 99%

Sixty Years (1957–2017) of Research on Toxoplasmosis in China—An Overview

et al. 2017

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Toxoplasma gondii is a ubiquitous zoonotic pathogen belonging to apicomplexan parasites. Infection in humans and animals may cause abortion and other severe symptoms under certain circumstances, leading to great economical losses and public health problems. T. gondii was first discovered in China in 1955 and the corresponding work was published in 1957. Since then, a lot of work has been done on this parasite and the diseases it causes. This review summarizes the major progress made by Chinese scientists over the last 60 years, and gives our perspectives on what should be done in the near future. A wide variety of diagnostic approaches were designed, including the ones to detect T. gondii specific antibodies in host sera, and T. gondii specific antigens or DNA in tissue and environmental samples. Further work will be needed to translate some of the laboratory assays into reliable products for clinic uses. Epidemiological studies were extensively done in China and the sero-prevalence in humans increased over the years, but is still below the world average, likely due to the unique eating and cooking habits. Infection rates were shown to be fairly high in meat producing animals such as, pigs, sheep, and chickens, as well as in the definitive host cats. Numerous subunit vaccines in the form of recombinant proteins or DNA vaccines were developed, but none of them is satisfactory in the current form. Live attenuated parasites using genetically modified strains may be a better option for vaccine design. The strains isolated from China are dominated by the ToxoDB #9 genotype, but it likely contains multiple subtypes since different ToxoDB #9 strains exhibited phenotypic differences. Further studies are needed to understand the general biology, as well as the unique features of strains prevalent in China.

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Section: Development Of Diagnostic Approachesmentioning

confidence: 99%

Sixty Years (1957–2017) of Research on Toxoplasmosis in China—An Overview

et al. 2017

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“…Recently, the recombinant antigens of secretory microneme proteins (MIC3), rhoptry proteins (ROPs), rhoptry neck proteins (RONs), and dense granule antigens (GRA) were developed as an alternative method for Toxoplasma sero-diagnosis for both humans and animals, and demonstrated to be more sensitive and specifi c at detecting T. gondii antibodies in multiple animal species compared to MAT and IFAT [33][34][35][36][37][38]. Specifi cally, Sun and colleagues reported >90% agreement between recombinant GRA1, GRA7 and Toxoplasma soluble antigens (TSA) for T. gondii antibody detection in chickens, with GRA7 showed higher sensitivity and specifi city than GRA1 and TSA [40]. To establish good epidemiological data of toxoplasmosis, additional other serological tests such as ELISA or recombinant antigen based diagnostic technique, bioassays and polymerase PCR based assays was recommended.…”

Section: Discussionmentioning

confidence: 99%

Toxoplasma Serostatus in Thai Free- Range Chickens: Prevalence and Two Diagnostic Methods

Udonsom¹,

Chaichan²,

Mahittikorn³

et al. 2017

Open J Trop Med

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Toxoplasma seroprevalence in free-range chicken, which directly feed from ground, is a good indicator for detecting an environment contamination by T. gondii oocysts. Many serological methods have been used for diagnosing Toxoplasma infection in animals including chicken. Selection of appropriate methods primarily depend on their sensitivity and specifi city as well as laboratory techniques, special reagent or tool requirements and regional prevalence status. The aims of this study were to determine the prevalence of T. gondii antibody in free-range chickens in Thailand and to compare two serodiagnostic methods, indirect fl uorescent antibody test (IFAT) and Modifi ed Agglutination Test (MAT). Six hundred free-range chicken sera were collected from 2 villages in Kanchanaburi Province, Thailand, 300 in 2014 and 300 in 2015. The age of chickens was between 3-6 mo, of which chickens of 2015 were older than of 2014. Collected sera were test for T. gondii IgG antibody by both methods. Over all Toxoplasma seroprevalence were 33.0% and 17.7% by IFAT and MAT, respectively. There was poor agreement between the two serological methods (kappa=0.09). IFAT in this study revealed higher positive percentage than MAT. Even though the way of living and environment in the present study was suitable for Toxoplasma transmission, the seroprevalence in Thai free-range chickens was low compared to other tropical regions such as Africa or South America. A low Toxoplasma prevalence was also found in humans, cats and other intermediate hosts in Thailand. Toxoplasmosis in Thailand causes low burden compare to other infectious diseases, however, consume well-cooked free-range chicken meat and proper practice during handling raw meat when cooking should be advocated to prevent and reduce this infection.

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“…We also modified the incubation temperature to 37°C, according to Sun et al . ( 2015 ). Plates were incubated for 1 h with serum samples and then for 45 min with the secondary antibody.…”

Section: Methodsmentioning

confidence: 99%

Meat juice serology for Toxoplasma gondii infection in chickens

et al. 2016

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Toxoplasma gondii is an important foodborne zoonosis. Free-range chickens are at particularly high risk of infection and are also excellent indicators of soil contamination by oocysts. In the present study, hearts of 77 free-range chickens were collected at slaughter. T. gondii meat juice enzyme-linked immunosorbent assay was performed with a commercial kit, following validation with positive controls, from experimentally infected chickens, and negative ones. Out of 77 samples, only 66 gave sufficient meat juice for serology. Of these, 24 (36.4%) were positive for T. gondii considering the 5*standard deviation values (calculated on the optical density of negative controls), while all the samples were negative considering sample/positive% values. Parasite-specific polymerase chain reaction was carried out on all samples obtained from heart tissue and none were positive for the presence of T. gondii DNA. Results would suggest that further study on the use of meat juice with a validated serological test to detect T. gondii in chickens could lead to widespread epidemiological studies in this important intermediate host. However, sample collection and test specificity require further evaluation.

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Evaluation of an indirect ELISA using recombinant granule antigen GRA1, GRA7 and soluble antigens for serodiagnosis of Toxoplasma gondii infection in chickens

Cited by 25 publications

References 26 publications

Sixty Years (1957–2017) of Research on Toxoplasmosis in China—An Overview

Sixty Years (1957–2017) of Research on Toxoplasmosis in China—An Overview

Toxoplasma Serostatus in Thai Free- Range Chickens: Prevalence and Two Diagnostic Methods

Meat juice serology for Toxoplasma gondii infection in chickens

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