Evaluation of an immunoradiometric assay specific for the CK-MB isoenzyme for detection of acute myocardial infarction

Al-Sheikh, W.; Heal, A.; Pefkaros, Kyriacos; Piña, Ileana L.; Serafini, Aldo N.; Ihmedian, Ismail H.; Ashkar, Fuad S.

doi:10.1016/0002-9149(84)90180-2

Cited by 11 publications

(2 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Double-antibody techniques are now available (10)(11)(12)(13)(14)(15)(16)(17)(18)(19). Double-antibody techniques are now available (10)(11)(12)(13)(14)(15)(16)(17)(18)(19).…”

Section: Discussionmentioning

confidence: 99%

“…This difference is useful as a non-invasive index of coronary reperfusion (9). The recent introduction of the immunoassay "sandwich" methods, employing anti-monomer M and anti-monomer B antibodies for specific detection of creatine kinase MB, offers an alternative measurement of MB isoenzyme, which quantifies MB mass concentration instead of MB catalytic activity concentration (10)(11)(12)(13)(14)(15)(16)(17)(18). However, our published results showed that an exact comparison between MB activity and mass concentration measurements can be difficult (19).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Short Communication

1988

Clinical Chemistry and Laboratory Medicine

View full text Add to dashboard Cite

Serum creatine kinase MB isoenzyme time-activity curves are useful for the assessment of coronary rcperfusion after acute myocardial infarction. The purpose of this study was to compare serum creatine kinase MB catalytic activity with mass concentration for the determination of coronary reflow after therapeutic thrombolysis. Creatine kinase MB mass was determined immunoenzymometrically. Creatine kinase MB catalytic activity concentration was determined by electrophoresis. Serum was collected every 4 hours for 96 hours in two groups of myocardial infarction patients: A (n = 10), urokinase induced reperfusion; B (n = 10), conventional therapy without urokinase. Peaks of mass and activity occurred at similar limes in groups A and B. Both were significantly earlier in the urokinase treated patients. The maximal rate of increase of creatine kinase MB (based on either mass or catalytic activity) was threefold greater in the urokinase group. There are no important differences between the behaviour of creatine kinase measured as catalytic concentration or as mass concentration. Mass concentration is therefore equally useful as an indicator of coronary reperfusion.

show abstract

“…Double-antibody techniques are now available (10)(11)(12)(13)(14)(15)(16)(17)(18)(19). Double-antibody techniques are now available (10)(11)(12)(13)(14)(15)(16)(17)(18)(19).…”

Section: Discussionmentioning

confidence: 99%