Evaluation of an immunochromatographic assay: Giardia-Strip® (Coris BioConcept) for detection of Giardia intestinalis in human fecal specimens

Nguyen, T. K. T.; Kherouf, H.; Blanc-Pattin, V.; Allais, E.; Chevalier, Y.; Richez, A.; Ramade, C.; Peyron, François

doi:10.1007/s10096-011-1332-9

Cited by 9 publications

(3 citation statements)

References 17 publications

(11 reference statements)

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“…As in other European countries. Microscopic examination remains the method of choice for the detection of G. duodenalis [38][39] . Most often, G. duodenalis was diagnosed in rural areas, which is confirmed by other researchers [40][41] .…”

Section: Discussionmentioning

confidence: 99%

Giardiasis in children: molecular genotyping, growth and calprotectin levels

Сорокман¹,

Sokolnyk²,

POPELYUK³

et al. 2019

Arch Balk Med Union

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La giardiase chez les enfants: le génotype moléculaire, la croissance des enfants et la calprotectine fécaleIntroduction. La giardiase est la plus sévère infection parasitaire de l'intestin humain. L'objectif de l'étude a été d'investiguer la fréquence de la giardiase, trouver son génotype, estimer la croissance et déterminer le niveau de la calprotectine fécale chez les enfants. Matériel et méthodes. On a utilisé la méthode directe microscopique de 688 enfants, âgés de 6 à 18 ans pour G. duodenalis. Deux groupes se sont constitués: Groupe I -avec un test positif pour G. duodenalis (n = 90); groupe II -enfants avec un test négatif (n = 110). On a effectué pour ces enfants, le dosage génétique, l'anthropométrie et la calprotectine fécale. Résultats. Sur ces 688 enfants examinés pour G. duodenalis, 90 ont donné un résultat positif pour ABSTRACT Introduction. G iardiasis is the most frequently reported human intestinal parasitic infection. The objective of the study was to investigate the frequency of giardiasis, carry out the genotyping, estimate the growth and determine the level of fecal calprotectin in children. Material and methods. 688 children aged 6-18 years were examined for Giardia duodenalis by direct microscopy. Two groups were formed: group I -children with a positive test for Giardia duodenalis (n = 90); group II -children with a negative test (n = 110). Genetic examination, anthropometry and fecal calprotectin (FC) evaluation were carried out in these children.Results. Out of the 688 children examined, 90 had a positive result (G. duodenalis (+)). The leading clinical feature of G. duodenalis infection (+) was abdominal pain, followed by nausea and diarrhea. The FC content in the feces of the group I was significantly

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Section: Discussionmentioning

confidence: 99%

Giardiasis in children: molecular genotyping, growth and calprotectin levels

Сорокман¹,

Sokolnyk²,

POPELYUK³

et al. 2019

Arch Balk Med Union

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“…ICT provides comparable sensitivities and specificities to CM and ELISA [ 39 , 40 ], but only detects half of PCR-positive samples at best [ 41 ]. In addition, a number of evaluation studies have revealed variable rates of ICT false-negative and false-positive results [ 42 , 43 ]. In our set of samples we estimated an ICT false-positive rate of nearly 5%, clearly indicating that ICT must be interpreted with caution and may require confirmation by a more sensitive and specific technique in certain cases.…”

Section: Discussionmentioning

confidence: 99%

Molecular Genotyping of Giardia duodenalis Isolates from Symptomatic Individuals Attending Two Major Public Hospitals in Madrid, Spain

et al. 2015

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BackgroundThe flagellate protozoan Giardia duodenalis is an enteric parasite causing human giardiasis, a major gastrointestinal disease of global distribution affecting both developing and industrialised countries. In Spain, sporadic cases of giardiasis have been regularly identified, particularly in pediatric and immigrant populations. However, there is limited information on the genetic variability of circulating G. duodenalis isolates in the country.MethodsIn this longitudinal molecular epidemiological study we report the diversity and frequency of the G. duodenalis assemblages and sub-assemblages identified in 199 stool samples collected from 184 individual with symptoms compatible with giardiasis presenting to two major public hospitals in Madrid for the period December 2013–January 2015. G. duodenalis cysts were initially detected by conventional microscopy and/or immunochomatography on stool samples. Confirmation of the infection was performed by direct immunofluorescence and real-time PCR methods. G. duodenalis assemblages and sub-assemblages were determined by multi-locus genotyping of the glutamate dehydrogenase (GDH) and β-giardin (BG) genes of the parasite. Sociodemographic and clinical features of patients infected with G. duodenalis were also analysed.Principal findingsOf 188 confirmed positive samples from 178 giardiasis cases a total of 124 G. duodenalis isolates were successfully typed at the GDH and/or the BG loci, revealing the presence of sub-assemblages BIV (62.1%), AII (15.3%), BIII (4.0%), AI (0.8%), and AIII (0.8%). Additionally, 6.5% of the isolates were only characterised at the assemblage level, being all of them assigned to assemblage B. Discordant genotype results AII/AIII or BIII/BIV were also observed in 10.5% of DNA isolates. A large number of multi-locus genotypes were identified in G. duodenalis assemblage B, but not assemblage A, isolates at both the GDH and BG loci, confirming the high degree of genetic variability observed in other molecular surveys. BIV was the most prevalent genetic variant of G. duodenalis found in individuals with symptomatic giardiasis in the population under study.ConclusionsHuman giardiasis is an ongoing public health problem in Spain affecting primarily young children under four years of age but also individuals of all age groups. Our typing and sub-typing results demonstrate that assemblage B is the most prevalent G. duodenalis assemblage circulating in patients with clinical giardiasis in Central Spain. Our analyses also revealed a large genetic variability in assemblage B (but not assemblage A) isolates of the parasite, corroborating the information obtained in similar studies in other geographical regions. We believe that molecular data presented here provide epidemiological evidence at the population level in support of the existence of genetic exchange within assemblages of G. duodenalis.

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“…Many studies reported that human stool samples tested with the ImmunoCardSTAT! ® , RIDAQUICK ® Combi and Duo-Strip ® kits yielded false negatives, and this was due to the presence of low numbers of Giardia cysts in the fecal specimens examined [8,16,17,19,20].…”

Section: Resultsmentioning

confidence: 99%