Evaluation of an Enzyme Immunoassay for Detection of Dengue Virus NS1 Antigen in Human Serum

Abstract

We evaluated a one-step sandwich-format microplate enzyme immunoassay for detecting dengue virus NS1 antigen (Ag) in human serum by use of Platelia Dengue NS1 Ag kits (Bio-Rad Laboratories, Marnes La Coquette, France). We collected 299 serum samples from patients with dengue disease and 50 serum samples from patients not infected with dengue virus. For the 239 serum samples from patients with acute infections testing positive by reverse transcription-PCR and/or virus isolation for one of the four dengue virus serotypes, the sensitivity of the Platelia Dengue NS1 Ag kit was 88.7% (95% confidence interval, 84.0% to 92.4%). None of the serum samples from patients not infected with dengue virus tested positive with the Platelia Dengue NS1 Ag kit. A diagnostic strategy combining the Platelia Dengue NS1 Ag test for acute-phase sera and immunoglobulin M capture enzyme-linked immunosorbent assay for early-convalescent-phase sera increased sensitivity only from 88.7% to 91.9%. Thus, NS1 antigen detection with the Platelia Dengue NS1 Ag kit could be used for first-line testing for acute dengue virus infection in clinical diagnostic laboratories.

Evaluation of Nonstructural 1 Antigen Assays for the Diagnosis and Surveillance of Dengue in Singapore

“…Dussart et al (2006) reported a similar finding for the performance of the Platelia Dengue NS1 Antigen ELISA assay. Though our analysis showed reduced sensitivity of Dengue Early assay for the detection of DENV3 serotype, more samples are needed to confirm this observation.…”

“…Evaluation of these assays (Dussart et al 2006, Kumarasamy et al 2007, Blacksell et al 2008, Lapphra et al 2008) has demonstrated high sensitivity and specificity for DENV detection, particularly during the acute phase of the disease. In this study, we evaluated the performance of three commercially available dengue NS1 antigen assays, in comparison with other widely used dengue diagnostic assays, to determine the usefulness of NS1 antigen assays in early diagnosis of dengue in a primary healthcare setting in Singapore.…”

Co-Infection of Dengue Virus by Serotypes 1 and 4 in Patient From Medium Sized City From Brazil

“…This assay provides additional dengue diagnostic tool to the existing approaches of PCR, antibody capture ELISA and virus isolation 5,6,10,12,13,20,24,25,28 . Even though NS1 is a sensitive methodology for the detection of DENV it should never be used as the only methodology for DENV detection because it doesn't identify different serotypes 39 .…”

Evaluation of the Dengue NS1 Ag Strip®for Detection of Dengue Virus Antigen inAedes aegypti(Diptera: Culicidae)

“…NS1 is a structural protein that is produced in both membrane-associated and secreted forms. It is involved in viral replication and has been shown to correlate with viremia (Libraty et al 2002, Dussart et al 2006 Environmental Health Institute, National Environmental Agency, Singapore, Singapore. DOI: 10.1089DOI: 10.…”