Evaluation of an ELISA for detection of antibodies to Babesia bovis in cattle in Australia and Zimbabwe

Molloy, J.B.; Bowles, P.M.; Bock, R.E.; Turton, J. A.; Katsande, Tendai C.; Katende, J.M.; Mabikacheche, L.G.; Waldron, Susan J.; Blight, GW; Dalgliesh, R.J.

doi:10.1016/s0167-5877(97)00063-9

Cited by 24 publications

(13 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast, the IFAT method is in disagreement with the ELISA and EIS tests for two out of the four samples tested (Table 2). These results are not unexpected given the subjective nature of the IFAT test [4,7], and the fact that both, ELISA and EIS were designed for detecting only specific antibodies against the rRAP-1/CT-STR antigen. Therefore, although the results suggest that EIS could also be used as a method for the detection of antibodies in cattle samples using B. bovis rRAP-1/CT-STR protein immobilized on gold substrate, more field cattle serum samples needs to be analysed in future work comparing these three antibody detection methods using an statistically validated design.…”

Section: Detection Of Antibodies Against B Bovis Rrap-1/ct-str Protementioning

confidence: 84%

“…Acute infections are usually diagnosed by microscopic examination of blood smears, whereas subclinical chronic infections can be identified serologically [2]. In addition, the indirect fluorescent antibody test (IFAT) is one of the most commonly used methods for the diagnosis of B. bovis infection [3,4] and has been applied in epidemiological studies in a number of countries [5,6], but IFAT was rendered cumbersome and subjective [4,7].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

An impedance spectroscopy method for the detection and evaluation of Babesia bovis antibodies in cattle

Silva¹,

Helali²,

Esseghaier³

et al. 2008

Sensors and Actuators B: Chemical

View full text Add to dashboard Cite

Section: Detection Of Antibodies Against B Bovis Rrap-1/ct-str Protementioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

An impedance spectroscopy method for the detection and evaluation of Babesia bovis antibodies in cattle

Silva¹,

Helali²,

Esseghaier³

et al. 2008

Sensors and Actuators B: Chemical

View full text Add to dashboard Cite

“…Serological assays are currently most likely to meet these requirements, as B. bovis infection finally leads to an asymptomatic carrier animal state in which the parasite commonly escapes direct detection by PCR, reverse line blot hybridization (RLB), or Giemsa-stained blood smears (10,23). A number of serological methods have been established for the detection of B. bovis-specific antibodies, of which ELISA is considered the most advantageous for epidemiological investigations, as it offers greater sensitivity and objectivity and can be easily adapted to test large numbers of serum samples (3,4,6,7,8,11,20,21,27,31,35,37). Furthermore, this format is amenable to the use of recombinant antigens, which makes it independent of the necessity to produce large amounts of parasites in vivo or in vitro, facilitates standardization, and has the potential to overcome limitations caused by cross-reactivity (22,32).…”

Section: Resultsmentioning

confidence: 99%

Validation and Field Evaluation of a Competitive Enzyme-Linked Immunosorbent Assay for Diagnosis of Babesia bovis Infections in Argentina

Dominguez

Echaide²,

Echaide³

et al. 2012

Clin Vaccine Immunol

View full text Add to dashboard Cite

ABSTRACTInfections byBabesia bovislimit cattle production and cause important economic losses in tropical and subtropical areas around the world. Monitoring of calf sera can be used to detect unprotected cattle herds and to decide on strategic control measures, as well as for epidemiological studies. Merozoite surface antigen 2c (MSA-2c) is an immunodominant surface protein expressed inB. bovismerozoites and sporozoites and contains B-cell epitopes that are conserved among geographic isolates. A monoclonal antibody against recombinant MSA-2c (rMSA-2c) was previously shown to inhibit the binding of anti-B. bovisantibodies to a parasite B-cell epitope in a competitive enzyme-linked immunosorbent assay (cELISA) format. In the work at hand, the parameters of this cELISA were reevaluated and adjusted when necessary, and a cutoff value was determined by receiver operator characteristic (ROC) curve analysis of a total of 357 bovine sera of known reactivity, as assessed by indirect immunofluorescence assay (IFAT). The established rMSA-2c cELISA demonstrated a specificity of 98% and a sensitivity of 96.2%. An additional set of 303 field bovine sera from regions where ticks are endemic and tick-free regions of Argentina was tested by both rMSA-2c cELISA and IFAT, and the results were shown to be in very good agreement (kappa index, 0.8325). The performance shown by rMSA-2c cELISA in the detection ofB. bovis-specific antibodies and its suitability for standardization and large-scale production, as well as the possibility of its application in most veterinary diagnostic laboratories, make the assay a powerful tool for the surveillance of herd immunity as a strategic measure for the control of bovine babesiosis.

show abstract

“…Other serological methods that have been developed and utilized in the past include an IIF assay (6) and a number of ELISAs (1,3,12,13,17). Although these assays allow the detection of infected cattle, they all have limitations to some degree in specificity, sensitivity, reliability, or, in the case of IIF, subjective interpretation and low throughput.…”

Section: Discussionmentioning

confidence: 99%

Validation of a Competitive Enzyme-Linked Immunosorbent Assay for Detection of Antibodies against Babesia bovis

Goff

Molloy

Johnson

et al. 2006

Clin Vaccine Immunol

View full text Add to dashboard Cite

A previously developed competitive enzyme-linked immunosorbent assay (cELISA) based on a speciesspecific, broadly conserved, and tandemly repeated B-cell epitope within the C terminus of rhoptry-associated protein 1 of Babesia bovis was refined and validated for use internationally. Receiver operating characteristic analysis revealed an assay with a specificity and positive predictive value of 100% and a sensitivity of 91.1%, with various negative predictive values depending on the level of disease prevalence. The cELISA was distributed to four different laboratories, along with a reference set of 100 defined bovine sera, including knownpositive, known-negative, and field samples. Pairwise concordances among the four laboratories ranged from 94% to 88%. Analysis of variance of the resulting optical densities and a test of homogeneity indicated no significant difference among the laboratories. Overall, the cELISA appears to have the attributes necessary for international application.

show abstract

Evaluation of an ELISA for detection of antibodies to Babesia bovis in cattle in Australia and Zimbabwe

Cited by 24 publications

References 7 publications

An impedance spectroscopy method for the detection and evaluation of Babesia bovis antibodies in cattle

An impedance spectroscopy method for the detection and evaluation of Babesia bovis antibodies in cattle

Validation and Field Evaluation of a Competitive Enzyme-Linked Immunosorbent Assay for Diagnosis of Babesia bovis Infections in Argentina

Validation of a Competitive Enzyme-Linked Immunosorbent Assay for Detection of Antibodies against Babesia bovis

Contact Info

Product

Resources

About