Evaluation of an ELISA - PPD for the diagnosis of bovine tuberculosis in field trials in Brazil

Lilenbaum, Walter; Ribeiro, Elisângela; Souza, Guilherme Nunes de; Moreira, É. C.; Fonseca, Leila S.; Ferreira, María Auxiliadora Pantoja; Schettini, Jorge

doi:10.1053/rvsc.1998.0229

Cited by 29 publications

(33 citation statements)

References 8 publications

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“…Similar results have been described for cattle (13,31). In the present study, this effect was observed for four of six antigens following SICTT.…”

Section: Discussionsupporting

confidence: 92%

“…Also, the presence of antibody provides only indirect evidence of TB infection in skin testnegative goats that did not undergo postmortem examination. Nevertheless, a large number of studies have documented correlations between antibodies and TB lesions and/or bacterial loads in cattle and other species (13,14,17,28,31,33,34,37,44,45). The Enferplex test has previously been shown to detect infected cattle with lesions that were missed by the SICTT (17).…”

Section: Discussionmentioning

confidence: 99%

“…Since skin and serology tests measure different arms of the immune response, detection of specific antibodies has the potential to identify infected animals not revealed by skin tests. Several studies have documented antibody responses in goats and other animals that are negative in CMI tests (2,(12)(13)(14).…”

mentioning

confidence: 99%

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Serological Analysis of Tuberculosis in Goats by Use of the Enferplex Caprine TB Multiplex Test

O’Brien¹,

Whelan²,

Clarke³

et al. 2017

Clin Vaccine Immunol

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Tuberculosis in goats is usually diagnosed clinically, at postmortem, or by a positive skin test. However, none of these approaches detects all infected animals. Serology offers an additional tool to identify infected animals missed by current tests. We describe the use of the Enferplex Caprine TB serology test to aid the management of a large dairy goat herd undergoing a tuberculosis breakdown. Initial skin and serology testing showed that IgG antibodies were present in both serum and milk from 100% of skin test-positive animals and in serum and milk from 77.8 and 95.4% of skin test-negative animals, respectively. A good correlation was observed between serum and milk antibody levels. The herd had been vaccinated against Mycobacterium avium subsp. paratuberculosis, but no direct serological crossreactions were found. Subsequent skin testing revealed 13.7% positive animals, 64.9% of which were antibody positive, while 42.1% of skin test-negative animals were seropositive. Antibody responses remained high 1 month later (57.1% positive), and the herd was slaughtered. Postmortem analysis of 20 skin test-negative goats revealed visible lesions in 6 animals, all of which had antibodies to six Mycobacterium bovis antigens. The results provide indirect evidence that serology testing with serum or milk could be a useful tool in the diagnosis and management of tuberculosis in goats.KEYWORDS tuberculosis, antibodies, goats T uberculosis (TB) due to infection by Mycobacterium bovis is a major problem in cattle in the United Kingdom, causing huge financial losses, as well as being a significant zoonosis risk. The presence of significant levels of TB in wildlife vectors such as the badger has led to trial culling or vaccination of these animals in high-risk areas such as southwestern England and Wales, respectively. Spillover hosts such as sheep, goats, deer, and alpacas also present a risk of spreading TB (1). Currently, control programs involving cattle are focused on detecting cell-mediated immunity (CMI) through the use of tuberculin skin testing and gamma interferon (IFN-␥) tests. In goats, the single intradermal comparative tuberculin test (SICTT) and the single intradermal test (SIT) are used to detect infection (2-5). Published estimates of SICTT sensitivity range from 42.7 to 83.7%, while those of SIT range from 44.6 to 93.8% (2, 3, 5). However, a recent study adopting bacteriology as the gold standard showed the sensitivity of the SIT by the severe interpretation to be as low as 43.9% and to decrease to 38.8% by the standard interpretation, while the sensitivity of the SICTT ranged between 21.3 and 7%, depending of the interpretation criterion used (6).Recent studies also showed that when goats are coinfected with Mycobacterium avium subsp. paratuberculosis, the sensitivity and specificity of the TB skin tests appear to be adversely affected, with a substantial proportion of M. bovis-infected animals going undetected (2-4, 7-11). Since skin and serology tests measure different arms of

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“…Similar results have been described for cattle (13,31). In the present study, this effect was observed for four of six antigens following SICTT.…”

Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Serological Analysis of Tuberculosis in Goats by Use of the Enferplex Caprine TB Multiplex Test

O’Brien¹,

Whelan²,

Clarke³

et al. 2017

Clin Vaccine Immunol

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“…When the test is read 72 h later, animals that have been exposed to mycobacterial infection show an antibody response, visible as a swelling at the injection site. The test is estimated to identify 80.9% of infected animals and 90.2% of uninfected animals correctly (J. P. Raath, unpublished data), which is similar to tuberculin-test sensitivity and specificity observed in cattle (80-91% and 95-100%, respectively; Lilenbaum et al 1999, Llamazares et al 1999, Ameni et al 2000. Cross-reactivity of bovine tuberculin with immune responses to other mycobacteria, such as Mycobacterium avium, can yield false-positive results.…”

Section: Buffalo Capture and Disease Surveymentioning

confidence: 53%

Hidden Effects of Chronic Tuberculosis in African Buffalo

Jolles

Cooper²,

Levin

2005

Ecology

102

121

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Abstract. Infectious diseases can bring about population declines and local host extinctions, contributing significantly to the global biodiversity crisis. Nonetheless, studies measuring population-level effects of pathogens in wild host populations are rare, and taxonomically biased toward avian hosts and macroparasitic infections. We investigated the effects of bovine tuberculosis (bTB), caused by the bacterial pathogen Mycobacterium bovis, on African buffalo (Syncerus caffer) at Hluhluwe-iMfolozi Park, South Africa. We tested 1180 buffalo for bTB infection between May 2000 and November 2001. Most infections were mild, confirming the chronic nature of the disease in buffalo. However, our data indicate that bTB affects both adult survival and fecundity. Using an age-structured population model, we demonstrate that the pathogen can reduce population growth rate drastically; yet its effects appear difficult to detect at the population level: bTB causes no conspicuous mass mortalities or fast population declines, nor does it alter host-population age structure significantly. Our models suggest that this syndrome-low detectability coupled with severe impacts on population growth rate and, therefore, resilience-may be characteristic of chronic diseases in large mammals.

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“…Em relação à TB, no RS, ALMEIDA et al (1997) estimaram uma prevalência de 1,33%. Em São Paulo, observou-se uma prevalência de 15,64% (MELO et al, 1997), enquanto, no Rio de Janeiro, LILENBAUM et al (1999), observaram uma taxa de infecção de 12,7% em 1622 animais de 13 propriedades com história prévia de TB. A variação na prevalência da TB nas diferentes regiões pode estar relacionada a diversos fatores como fonte de aquisição de animais, manejo, clima e ao serviço de diagnóstico da tuberculose em cada propriedade.…”

Section: Dois Mil Cento E Dezenove Bovinos Adultos Pertencentes a 156unclassified

Prevalência de tuberculose, brucelose e infecções víricas em bovinos leiteiros do município de Passo Fundo, RS

et al. 2004

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Dois mil cento e dezenove bovinos adultos pertencentes a 156 propriedades leiteiras distribuídas em 22 localizadas do município de Passo Fundo foram testados para tuberculose bovina (TB) e brucelose bovina (BB). A prevalência de anticorpos contra o vírus da diarréia viral bovina (BVDV), vírus da leucose enzoótica bovina (BLV) e herpesvírus bovino tipo 1 (BHV-1) foi estimada por amostragem aleatória em 28 dessas propriedades. Para tanto, considerou-se prevalência acima de 30% para essas infecções e intervalo de confiança de 90%, e sorteou-se 204 amostras de soro sangüíneo. Entre os 2.119 animais testados, 32 (1,51%) foram positivos para TB e 26 (1,22%) foram positivos para BB. Os exames sorológicos dos animais pertencentes às 28 propriedades indicaram animais positivos para BHV-1, BVDV e BLV em 22 (92,85%), 19 (67,85%) e 17 (60,71%) propriedades, respectivamente; 11 (39,28%) propriedades apresentaram animais soropositivos tanto para BHV-1, BVDV e BLV; em 1 (3,57%) propriedade apenas todos os animais eram soronegativos para essas infecções.

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Evaluation of an ELISA - PPD for the diagnosis of bovine tuberculosis in field trials in Brazil

Cited by 29 publications

References 8 publications

Serological Analysis of Tuberculosis in Goats by Use of the Enferplex Caprine TB Multiplex Test

Serological Analysis of Tuberculosis in Goats by Use of the Enferplex Caprine TB Multiplex Test

Hidden Effects of Chronic Tuberculosis in African Buffalo

Prevalência de tuberculose, brucelose e infecções víricas em bovinos leiteiros do município de Passo Fundo, RS

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