Evaluation of an electrochemical biosensor for uric acid measurement in human whole blood samples

Liao, Li Ting; Liao, Chi Chih; Liu, Chiu Ching; Yang, Ting Ya; Wang, Giueng Chueng

doi:10.1016/j.cca.2014.04.033

Cited by 24 publications

(13 citation statements)

References 14 publications

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“…Liao et al compared UA measurements utilizing an electrochemical detection device that used an electrode to oxidize UA, producing a measurable current proportional to UA concentration. Their study found that blood samples collected into a sodium citrate anticoagulant manifested 10.4% lower UA measurements as compared to a standard reference [8]. Further research has yielded conflicting results regarding the measurement of metabolites such as UA based on differences in sample collection and the assay used [9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

“…Recent investigations have focused on the long-term stability of metabolites in blood samples, as well as differences in metabolite concentrations based upon blood sample type [5][6][7][8]. Liu and Aa et al demonstrated that UA concentrations measured from plasma ethylenediaminetetraacetic acid (EDTA) samples were significantly higher than serum samples using gas chromatography and time-of-flight mass spectrometry [6].…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant properties of citric acid interfere with the uricase-based measurement of circulating uric acid

Ryan

Duryee

Hollins

et al. 2019

Journal of Pharmaceutical and Biomedical Analysis

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Background: Circulating uric acid (UA) is an important biomarker, not only in the detection and management of gout, but also in assessing the risk of related comorbidity. The impact of collection methods on clinical UA measurements has been the subject of limited study. After observing significant differences between UA concentrations of blood samples obtained by different collection tubes, we began examining the effects of exogenous tube components on measured UA concentrations. We aimed to: (1) demonstrate the variability in uricase-based UA measurements attributable to different collection methods and (2) identify factors influencing this variability.Methods: Blood samples from human subjects were collected using Serum Separator Tubes (SST tubes), Acid Citrate Dextrose (ACD) tubes, and Sodium Citrate (SC) tubes. Circulating UA concentrations were measured by chemistry analyzers utilizing the uricase method. Absorbance assays were run in order to determine the effects of citric acid, sodium citrate, and dextrose on measured absorbance in the presence of leuco crystal violet dye, hydrogen peroxide, and peroxidase. Statistical analyses-including Student's T tests and ANOVA-were used to compare results.Results: UA concentrations of blood samples collected in ACD tubes were significantly lower than those collected in SST tubes (P<0.01). Samples collected in SC tubes trended towards lower

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antioxidant properties of citric acid interfere with the uricase-based measurement of circulating uric acid

Ryan

Duryee

Hollins

et al. 2019

Journal of Pharmaceutical and Biomedical Analysis

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“…Light from optical fibre is collimated into parallel beam using Ocean Optics 74-UV collimating lens (3) to interact with solution in cuvette (4) as shown in Figure 5. Then, light from cuvette is collected using the collimating lens and delivered to Ocean Optics HR4000CG-UV-NIR high resolution spectrometer (5). Ocean Optics OceanView spectrometer operating software is used to obtain and process data from spectrometer.…”

Section: Methodsmentioning

confidence: 99%

“…Analysis of sensitivity and accuracy in this work was calculated using Beer Lambert law. The Beer-Lambert law defines the attenuation of light to the properties of the sample in terms of transmittance, T and absorbance, A as stated in (1) and (2)(3)(4)(5). In this work, the output light intensity after passing through cuvette without sample, Iref, and with sample, Io, is measured by using spectrometer.…”

Section: Theorymentioning

confidence: 99%

Uric acid detection in visible spectrum

et al. 2020

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The measurement of uric acid based on the optical absorption at visible light spectrum is investigated and tested. Sensing in the visible region was conducted for determination of suitable wavelength that produces high sensitivity and accuracy performance based on the Beer-Lambert law calculation. In this work, the uric acid is detected by detecting sodium urate as a product of chemical reaction between uric acid with sodium hydroxide buffer. The setup has been tested for uric acid concentration ranging from 15 mg/dL to 85 mg/dL. Three wavelengths have been analyzed which are 460 nm, 525 nm and 630 nm. Measured data at 460nm wavelength exhibits the highest sensitivity, which is 0.0012 (mg/dL)-with 86.51% accuracy. Detection of uric acid at visible light spectrum offers a low-cost sensor based on visible LEDs and photodiode is possible to be realized.

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“…Monitoring the level of uric acid (UA) in human physiological fluids is indispensable for the diagnosis of patients suffering from a range of disorders associated with altered purine metabolism [1]. Various electrochemical UA biosensors have emerged from laboratories, because of the advantages of simplicity, short response time, high sensitivity, and selectivity [2]. However, interference from common biological materials such as acetaminophen, dopamine, and ascorbic acid has been the most serious problem encountered when applying reagent based methods for uric acid measurement [3].…”

Section: Introductionmentioning

confidence: 99%

Voltammetric Determination of Uric Acid in Clinical Serum Samples Using DMF Modified Screen Printed Carbon Electrodes

Metto

Eramias

Gelagay

et al. 2019

International Journal of Electrochemistry

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Screen printed carbon electrodes (SPCEs) provide attractive opportunity for sensitive and selective determination target analytes in clinical samples. The aim of the current work was to develop SPCEs based sensor for the determination of uric acid in clinical serum samples. The electrodes were pretreated by soaking in N,N-dimethylformamide for 5 minutes followed by drying in an oven at 100°C for 20 mins. The effect of surface pretreatment was characterized using cyclic voltammetry. The current response of uric acid detection was improved by a factor of 3.5 in differential pulse voltammetric measurement compared to unmodified electrode. Under the optimized conditions, the sensor displayed two dynamic linear ranges 5-100 μM and 100-500 μM with correlation coefficient, R2, values of 0.98782 and 0.97876, respectively. The limit of detection and limit of quantification calculated using the dynamic linear range 5-100 μM were 1.9 x 10−7 M and 6.33 x 10−7 M, respectively. The developed sensor displayed well separated and discerned peaks for UA in presence of the potential interferent (ascorbic acid and citric acid). The electrode was successfully applied for the detection of very low level of UA in clinical serum samples in a phosphate buffer solution (pH = 7). The proposed sensor showed a very high reproducibility and repeatability with the relative standard deviation of 0.9%. In conclusion, a simple and low cost sensor based on SPCEs is developed for sensitive and selective detection of uric acid in clinical samples.

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Evaluation of an electrochemical biosensor for uric acid measurement in human whole blood samples

Cited by 24 publications

References 14 publications

Antioxidant properties of citric acid interfere with the uricase-based measurement of circulating uric acid

Antioxidant properties of citric acid interfere with the uricase-based measurement of circulating uric acid

Uric acid detection in visible spectrum

Voltammetric Determination of Uric Acid in Clinical Serum Samples Using DMF Modified Screen Printed Carbon Electrodes

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