Evaluation of an automated system of immunofluorescence analysis in daily practice

Mathiaux, François; Barrot, Amandine; Elong, Cécile; Parent, Xavier

doi:10.1684/abc.2018.1364

Cited by 1 publication

(4 citation statements)

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“…[ 15 ] Previous studies showed that IIF-ANCA was easy to cover up with high titer ANA. [ 16 , 17 ] IIF-ANCA display lower specificities. Therefore, ANA was generally considered to be an interference factor for IIF-ANCA, and the clinical significance of ANA in AAV patients was usually ignored.…”

Section: Discussionmentioning

confidence: 99%

“…IIF was the first method used and is still the most commonly used ANCA and ANA at present [15] . Previous studies showed that IIF-ANCA was easy to cover up with high titer ANA [16,17] . IIF-ANCA display lower specificities.…”

Section: Discussionmentioning

confidence: 99%

“…Reticular/AMA (AC-21) was not detected in any of the three ANCA patterns. There were no statistically differences in terms of RR (AC-23), fibrillar (AC- 15,16,17), and polar/Golgi-like (AC-22).…”

Section: Correlation Between Iif-ana Cytoplasmic Patterns and Iif-anc...mentioning

confidence: 93%

“…As the most widely used biomarker of autoimmune diseases, HEp-2 cell cytoplasmic pattern of ANA is of great significance. In August 2014, the International Consensus on antinuclear antibody pattern meeting made clear that cytoplasmic patterns were categorized as a major group and required patterns including fibrillar (AC- 15,16,17), speckled 19,20), reticular/AMA (AC-21), polar/Golgi-like (AC-22), and rods and rings (RR) (AC-23). [7] Studies on HEp-2 cell cytoplasmic patterns mainly focus on autoimmune diseases, and the clear patterns are RR, polar/ Golgi-like and reticular/AMA at present.…”

Section: Introductionmentioning

confidence: 99%

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Clinical significance of HEp-2 cell cytoplasmic patterns in anti-neutrophil cytoplasmic antibody associated vasculitis

Zhang

Bao

et al. 2022

Medicine

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The study was to investigate the clinical characteristics and significance of antinuclear antibody (ANA) cytoplasmic patterns in ANCA-associated vasculitis (AAV) from Southwest China. A retrospective study including 232 AAV patients from Peoples Hospital of Deyang City was performed. These included 115 patients with ANA cytoplasmic pattern as observation group and 117 patients without ANA cytoplasmic pattern as control group. Chest involvement (60.00 vs 46.15, P = .035), cardiovascular involvement (5.21 vs 29.91, P < .001), and renal involvement (37.39 vs 77.78, P = .001) were different between groups. Total protein (69.55 vs 64.01, P < .001), triglyceride (1.41 vs 1.18, P = .023), mean cell volume (89.76 vs 87.59, P = .040), and estimated glomerular filtration rate (76.67 vs 50.87, P = .035) were higher in ANA cytoplasmic patterns group. Creatinine (73.00 vs 117.50, P = .011), white blood cell (6.93 vs 8.86, P = .001), platelet (196.0 vs 239.0, P = .017), anti-myeloperoxidase (2.44 vs 3.42, P = .042), and anti-proteinase 3 (1.00 vs 4.93, P = .007) were lower in this group. In multivariate analysis, creatinine (odds ratio [OR] = 1.21, 95% confidence interval [CI]: 1.06–1.38), triglyceride (OR = 1.97, 95% CI: 1.10–3.48), and anti-myeloperoxidase (OR = 1.64, 95% CI: 1.37–1.95) were independent risk factors of AAV renal involvement. Total protein (OR = .95, 95% CI: 0.91–0.99) was an independent protective factor of AAV renal involvement. Chi-square test showed that speckled pattern was different among anti-neutrophil cytoplasmic antibody patterns ( χ 2 = 18.526, P < .001). In summary, HEp-2 cell cytoplasmic patterns have certain clinical significance in AAV, which is a new exploration of the clinical value of ANA.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%