Evaluation of an antigen from Taeniacrassiceps cysticercus for the serodiagnosis of neurocysticercosis

Peralta, Regina Helena Saramago; Vaz, Adelaide José; Pardini, Alessandra Xavier; Macedo, Heloísa Werneck de; Machado, L. R.; De-Simone, Salvatore Giovanni; Peralta, José Mauro

doi:10.1016/s0001-706x(02)00092-x

Cited by 32 publications

(44 citation statements)

References 38 publications

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“…Devido à alta homologia dos antí-genos da larva da T. solium aos da T. crassiceps, esses últimos têm sido utilizados nos métodos imunológicos empregados para o diagnóstico da cisticercose. As técnicas ELISA e WB, empregando esses antígenos, têm apresentado elevadas sensibilidade e especificidade em amostras de soro 21,22 , líquido cefaloraquidiano 13 ou de sangue total 11 .…”

Section: Comissão De éTicaunclassified

Estudo soroepidemiológico da cisticercose humana em um município do Estado do Piauí, Região Nordeste do Brasil

et al. 2004

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Integrando as pesquisas sobre parasitoses na região do entorno do Parque Nacional Serra da Capivara, Piauí, Brasil, realizadas entre 1999 e 2001, o presente estudo tem como objetivo avaliar a situação epidemiológica da cisticercose humana no Município de João Costa, no Nordeste do Brasil. Foram obtidas informações clínico-epidemiológicas e coletadas amostras de sangue para testes sorológicos imunoenzimáticos (ELISA e Western blot), empregando cisticercos de Taenia crassiceps como antígeno. Na primeira etapa, em 1999, foram investigadas 169 pessoas com história confirmada ou suspeita de infecção/doença pelo complexo teníase-cisticercose, e seus familiares. Na análise, 13,6% das pessoas apresentaram soros reagentes para cisticercose pelo método ELISA. Na segunda etapa, em 2001, foram avaliadas 92 amostras de soro de indivíduos reativos para cisticercose detectados no primeiro momento e seus familiares, sendo que 24,0% das amostras de soro foram reagentes para cisticercose pelo ELISA, e 29,0%, pelo WB. Nessa mesma etapa, realizou-se inquérito coprológico em 701 pessoas, incluindo voluntários. A prevalência de parasitoses intestinais foi de 51,0%, tendo sido observada uma maior prevalência de protozoários (95,0%) em relação aos helmintos (5,0%). Os resultados do estudo indicam o caráter endêmico da cisticercose na área, além da elevada freqüência de protozooses intestinais.

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Section: Comissão De éTicaunclassified

Estudo soroepidemiológico da cisticercose humana em um município do Estado do Piauí, Região Nordeste do Brasil

et al. 2004

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“…In the non NC group we obtained positive results mainly with the Tso antigen. The specificity of the ELISA with Tcra-vf has been reported as 80% and 96% by BUENO et al 5 and BRAGAZZA et al 4 , respectively, while PERALTA et al 17 reported 98% specificity for ELISA with 14 kDa glycoprotein of T. crassiceps. In this study the Tcra-gp antigen showed similar sensitivity and better specificity than that of Tcra-vf, so Tcra-gp seems to be the best choice to be attempt in order to improve the specificity of ELISA for screening purpose and efforts are underway through purification of the specific and immunodominant antigens.…”

Section: Discussionmentioning

confidence: 96%

“…Some studies demonstrated that T. solium from pig infection and T. crassiceps which can be maintained in mice, share antigenic components, including those of low molecular mass peptides (18 and 14 kDa) 8,15 . T. crassiceps larvae, which are easily maintained in the laboratory by intraperitoneal passage through female BALB/c mice, were shown to be an important alternative source of such antigen components 5,17,22 .…”

Section: Neurocysticercosis (Nc)mentioning

confidence: 99%

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Serodiagnosis of neurocysticercosis in patients with epileptic seizure using ELISA and immunoblot assay

Ishida

Peralta

Livramento

et al. 2006

Rev. Inst. Med. trop. S. Paulo

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SUMMARYSera from 88 patients from Santa Catarina and São Paulo states of Brazil, with epileptic seizures who underwent cerebral computed tomography (CT) were analyzed for the detection of antibodies to T. solium cysticercus by ELISA and Immunoblot (IB) with the following antigens: Taenia solium cysticercus total saline (Tso), Taenia crassiceps cysticercus vesicular fluid (Tcra-vf) and T. crassiceps cysticercus glycoproteins (Tcra-gp). ELISA carried out with Tso, Tcra-vf and Tcra-gp antigens showed 95%, 90% and 80% sensitivities, respectively, and 68%, 85% and 93% specificities, respectively. In the epileptic patients group, ELISA positivity was 30%, 51% and 35% with Tso, Tcra-vf and Tcra-gp antigens respectively. Considering the IB as the confirmatory test, the positivity was 16% (14/88) in the epileptic patients total group and 22% (12/54) in the epileptic patients with positive CT and signals of cysticercosis. We found a significant statistical correlation among ELISA or IB results and the phase of the disease when any antigens were used (p < 0.05). We emphasize the need to introduce in the laboratory routine the search for neurocysticercosis (NC) in patients presenting with epileptic seizures because of the high risk of acquiring NC in our region and its potential cause of epilepsy.

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“…Crude extracts of whole T. solium or T. crassiceps cysticerci, crude extracts from particular components of the parasites (scolex, membrane or vesicular fluid), excretory-secretory products, purified antigens from crude extracts, recombinant proteins and synthetic peptides have been used in immunological tests to detect specific antibodies [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21] . Currently, the most reliable immunological test available for the immunodiagnosis of neurocysticercosis is an enzyme-linked immunoelectrotransfer blot (EITB) assay based on glycoprotein antigens prepared by lentil lectin affinity chromatography from an extract of intact cysts 6,16 .…”

mentioning

confidence: 99%

Evaluation of two Taenia solium cysticercal antigenic preparations (vesicular fluid and a glycoprotein fraction with affinity for lentil lectin) for the immunodiagnosis of neurocysticercosis by enzyme-linked immunosorbent assay (ELISA)

Suzuki

Rossi

2011

Arq. Neuro-Psiquiatr.

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Objective: To evaluate the performance of two antigenic preparations (vesicular fluid -VF and a glycoprotein fraction, LLa-Gp fraction, purified from a whole parasite extract by lentil lectin affinity chromatography) from Taenia solium cysticerci for the immunodiagnosis of neurocysticercosis. Method: Fifty-six cerebrospinal fluid (CSF) samples (22 from patients with neurocysticercosis and 34 from patients with other neurological disorders) and 57 serum samples (22 from patients with neurocysticercosis, 18 from patients with other infections and 17 from presumably healthy persons) were assayed for anticysticercal IgG antibodies with an enzyme-linked immunosorbent assay (ELISA). Results: The VF ELISA showed 100% sensitivity and specificity in CSF and serum samples, whereas the sensitivity and specificity of the LLa-Gp ELISA were, respectively, 90.9% and 97.1%, with the CSF samples and 95.5% and 100% with serum samples. There was no significant difference in the sensitivity and specificity of the two antigenic preparations used to screen CSF and serum samples. Conclusion: Considering the complexity and high cost of obtaining the LLa-Gp fraction, VF could be more suitable for screening specific antibodies by ELISA in CSF and serum samples from patients with neurocysticercosis.

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Evaluation of an antigen from Taeniacrassiceps cysticercus for the serodiagnosis of neurocysticercosis

Cited by 32 publications

References 38 publications

Estudo soroepidemiológico da cisticercose humana em um município do Estado do Piauí, Região Nordeste do Brasil

Estudo soroepidemiológico da cisticercose humana em um município do Estado do Piauí, Região Nordeste do Brasil

Serodiagnosis of neurocysticercosis in patients with epileptic seizure using ELISA and immunoblot assay

Evaluation of two Taenia solium cysticercal antigenic preparations (vesicular fluid and a glycoprotein fraction with affinity for lentil lectin) for the immunodiagnosis of neurocysticercosis by enzyme-linked immunosorbent assay (ELISA)

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