Evaluation of Amplified Ribosomal DNA Restriction Analysis for Identification of Acinetobacter Genomic Species

Dijkshoorn, Lenie; Harsselaar, Barbara van; Tjernberg, Ingela; Bouvet, Philippe; Vaneechoutte, Mario

doi:10.1016/s0723-2020(98)80006-4

Cited by 114 publications

(109 citation statements)

References 26 publications

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“…Besides that DNA-DNA hybridization is regarded as the gold standard, other molecular typing methods also have been developed and validated. It is recommended that amplified 16S rRNA gene restriction analysis (ARDRA)[4] and amplified fragment length polymorphism (AFLP)[5] are the most widely accepted methods. However, they are too laborious and far from being suitable for day-to-day diagnostic microbiology.…”

Section: Introductionmentioning

confidence: 99%

TheAcinetobacter baumanniigroup:a systemic review

Zhang

Qiao

2013

World Journal of Emergency Medicine

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BACKGROUND:The Acinetobacter baumannii group, including Acinetobacter baumannii, Acinetobacter genomospecies 3 and 13TU, is phenotypically indistinguishable and uniformly identified as Acinetobacter baumannii by laboratories of clinical microbiology. This review aimed to demonstrate the differences among them.METHODS:Literatures associated with the Acinetobacter baumannii group were identified and selected from PubMed databases and relevant journals.RESULTS:Acinetobacter genospecies 3 and 13TU possess a certain proportion in clinical isolates. There were considerable differences in epidemiologic features, clinical manifestations, antimicrobial resistances and therapeutic options among the Acinetobacter baumannii group. Compared with Acinetobacter genomospecies 3 and 13TU, Acinetobacter baumannii with a higher resistance to antimicrobial agents are easier to be treated inappropriately, and present a worse outcome in patients.CONCLUSION:The Acinetobacter baumannii group comprises three distinct clinical entities, and their clinical value are not equal.

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Section: Introductionmentioning

confidence: 99%

TheAcinetobacter baumanniigroup:a systemic review

Zhang

Qiao

2013

World Journal of Emergency Medicine

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“…ARDRA and other methods that involve fragment restriction after PCR amplification should be preferred over methods where restriction digest occurs before PCR, because the former option is less prone to contamination and more reproducible (Stackebrandt et al, 2002). et al, 1995) Species identification in clinical isolates (Vaneechoutte et al, 1995;Dijkshoorn et al, 1998;Chandra et al, 2002).…”

Section: Genotypic Methodsmentioning

confidence: 99%

Safety Assessment of Transgenic Organisms, Volume 4

2010

Harmonisation of Regulatory Oversight in Biotechnology

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io s a fe t y nv ir on me nt ag ri cu lt ur e bi os af et y g r ic u lt u r e e n vi r o n m e n t gr ic ul tu re bi os af et y en vi ro nm en t n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o io sa fe ty en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en v io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t nv ir on me nt ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm e g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a f gr ic ul tu re bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t a n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u io sa fe ty en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m nv ir on me nt ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a f gr ic ul tu re bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t a n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u io sa fe ty en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e nv ir on me nt ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y e g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a f ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et...

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“…The ARDRA method was carried out as described previously [18,33]. Briefly, the amplified 16S rRNA gene was obtained by Gene Amp PCR system 9700 (Applied Biosystems).…”

Section: Identification Of the Isolates By Ardramentioning

confidence: 99%

“…The fragments obtained by digestion with each enzyme were electrophoretically separated in 2.5% agarose gels. Species identification was done by comparing the profiles consisting of the combination of restriction patterns generated with the different enzymes to those of a library of profiles of strains of described named and unnamed species [18,33] (http://allserv.rug. ac.be/~mvaneech/ARDRA/Acinetobacter.html).…”

Section: Identification Of the Isolates By Ardramentioning

confidence: 99%

“…Molecular methods that have been developed and validated for identification of Acinetobacters include amplified 16S rRNA gene restriction analysis (ARDRA) [18], high-resolution fingerprint analysis by amplified fragment length polymorphism (AFLP) [19], ribotyping [20], tRNA spacer fingerprinting [21], restriction analysis of the 16S-23S rRNA intergenic spacer sequences [22], sequence analysis of the 16S-23S rRNA gene spacer region [12], and sequencing of the rpoB (RNA polymerase β-subunit) gene and its flanking spacers [23]. Currently, ARDRA and AFLP are the most widely used validated reference methods for species identification of Acinetobacters [18,19] The tremendous increase in sequencing, lead to partial or nearly complete sequence analysis of the 16S rRNA gene for Acinetobacter classification [24,25]. In addition, the 16S and 23S rRNA genes intergenic spacer (ITS) has been suggested to be a good candidate for bacterial species identification [26], since these regions have degrees of low intraspecies variation and high degrees of interspecies divergence [27,28].…”

Section: Introductionmentioning

confidence: 99%

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Value of phenotypic and genotypic identification of Acinetobacter baumannii isolates from two hospitals in Jordan

Bustami

Dakkak²,

Abu-Qatouseh³

et al. 2014

int. arab. j antimicrob. agents

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A total of 12 Acinetobacter baumannii isolates have been recovered from hospitalized patients at two hospitals in Jordan over two different periods of time (2006 and 2008). Phenotypic and biochemical characterization with antibiotic susceptibility testing indicated that all isolates were belonging to A. baumannii. A high degree of conservation of both the 16S-23S rRNA gene intergenic spacer (ITS) length and the ITS sequence was observed among the isolates, and their identities were further confirmed by amplified ribosomal DNA gene restriction analysis (ARDRA). The application of ITS sequence-based identification and ARDRA provide promising tools for the elucidation of the clinical significance of genospecies identification of A. baumannii.

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Evaluation of Amplified Ribosomal DNA Restriction Analysis for Identification of Acinetobacter Genomic Species

Cited by 114 publications

References 26 publications

TheAcinetobacter baumanniigroup:a systemic review

TheAcinetobacter baumanniigroup:a systemic review

Safety Assessment of Transgenic Organisms, Volume 4

Value of phenotypic and genotypic identification of Acinetobacter baumannii isolates from two hospitals in Jordan

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