Evaluation of amplified fragment length polymorphism and simple sequence repeats for tomato germplasm fingerprinting: utility for grouping closely related traditional cultivars

Abstract

Cultivated tomato (Solanum lycopersicum L.) germplasm shows limited genetic variation. Many DNA marker systems have been used for genetic diversity studies in wild and cultivated tomatoes, but their usefulness for characterizing phenotypic differences among very closely related cultivars remains uncertain. We have used 19 selected simple sequence repeat (SSR) markers and 7 amplified fragment length polymorphism (AFLP) primer combinations to characterize 48 cultivars of tomato, mainly traditional cultivars from the south-east of Spain. The main types were Solanum lycopersicum L. 'Muchamiel', 'De la pera', and 'Moruno'. The robustness of the dendrograms and the discrimination power reached with each marker type were similar. Unique fingerprinting even of the most closely related tomato cultivars could be obtained using a combination of some SSR and AFLP markers. A better grouping of the 'Muchamiel' cultivars was observed with SSR markers, whereas the grouping of cultivars of 'De la pera' type was best achieved with AFLPs. However, both types of markers adequately grouped cultivars of the main types, confirming the utility of SSR and AFLP markers for the identification of traditional cultivars of tomato.

Genetic Diversity in Tomato (Solanum lycopersicum) and Its Wild Relatives

“…Selection of new genotypes within heterogeneous cultivars (or selection of chance variance) resulted from spontaneous mutations, natural outcrossing or recombination of pre-existing genetic variation. Thus, S. lycopersicum found in Europe a secondary centre for diversification (García-Martínez, Andreani et al 2006). In the XIX th century, establishment of commercial routes and colonies contributed to spread the species worldwide (Diez and Nuez 2008).…”

Genetic diversity of cultivated and wild tomatoes revealed by morphological traits and SSR markers

“…Fortunately, the model tomato vegetable from the family Solanceae has abundant genomic information, including a complete reference genome sequence and a rapidly developing comparative genomic database (http://solgenomics.net/). Therefore, many molecular markers generated from the sequence database have been successfully applied for identification and genetic diversity analysis of tomato cultivars (Park et al, 2004;García-Martínez et al, 2006;Benor et al, 2008;Korir et al, 2014).…”

Genetic Variation in Bell Peppers (Capsicum annuum L.) Collected from Iraqi Local Markets by Using AFLP Marker

“…1). The decreasing or lack of genetic variation is due to the nature of the auto-pollination of plant classes and also to the genetic basis, as it is known that the plant species with auto-pollination (inoculation) such as tomato Lycopersicon esculentum (belonging to the family Solanaceae) showed the low rate in the morphological diversity in own genome [33], [34], [35]. Kochieva and Ryzhova [13] revealed that the genetic variation was low in the fruits of pepper, they observed that the morphological diversity rate was16.5% when using 9 pairs of primers for AFLP technique.…”