Evaluation of <i>In-Vitro</i> Anti-Tuberculosis Activity of <i>Tetrapleura tetraptera</i> Crude and Fractions on Multidrug Resistant <i>Mycobacterium tuberculosis</i>

Abstract

The management, control and elimination of tuberculosis (TB) have been difficult with the advent of HIV and cases of multidrug resistant (MDR-TB) tuberculosis. The cases of multidrug resistance to rifampicin and isoniazid pose greater challenges on first line and second line drugs to eliminate TB. The study is aimed at establishing anti-tuberculosis activity of Tetrapleura tetraptera against Mycobacterium tuberculosis and MDR-TB and the phytochemical present. The leaves of Tetrapleura tetraptera were collected, weighed, dried and pulverized to powder. The pulverized leaves of Tetrapleura tetraptera were subjected to 70% methanol extraction and screened for phytochemical. The crude extract was further purified into fractions using silica gel and thin layer chromatography techniques. M. tuberculosis and MDR-TB were obtained from positive acid fast bacilli sputa of TB patients and confirmed using GeneXpert to differentiate genotypic drug susceptible M. tuberculosis and MDR-TB. The sputa were digested using sodium hydroxide-cysteine technique and cultured in Middlebrook 7H9. The crude extract and fractions were screened for anti-tuberculosis activity using tetrazolium microtitre plate assay. The results showed that Tetrapleura tetraptera crude had activities against M. tuberculosis at 7.4 ± 0 mg/ml and 27.5 ± 0 mg/ml for MDR-TB. One of the fractions inhibited the growth of M. tuberculosis at 0.

Synergistic activity of Tetrapleura tetraptera and Abrus precatorius fractions extract against Streptococcus pneumoniae and Mycobacterium tuberculosis

“…[38] [36] [25] [13] Tetrapleura tetraptera possess anti-tuberculosis activity. [37] The phytochemical compositions of the T. tetraptera Adeleye et al [31] Ogugor et al [39] and A.…”