Evaluation of ameliorative effect of sodium nitrate in experimental model of streptozotocin-induced diabetic neuropathy in male rats

Abstract

Objective. Diabetes induces sensory symptoms of neuropathy as positive (hyperalgesia), negative (hypoalgesia), or both.Methods. In the present study, fifty male Wistar rats were allocated to five groups: control, control+nitrate, diabetes, diabetes+insulin, and diabetes+nitrate. Thirty days after diabetes confirmation, insulin (2-4 U/day) was injected subcutaneously in diabetes+insulin group and nitrate (100 mg/l) was added into drinking water of the control+nitrate and diabetes+nitrate groups for a period of 2 months. In order to assess the mechanical and thermal algesia, tail immersion, hot plate, and von Frey tests were performed. The serum insulin levels were determined with insulin ELISA Kit. Serum level of NOx was determined by the Griess method.Results. Both thermal and mechanical nociceptive thresholds showed a significant decrease (p<0.05) which was followed by a significant increase (p<0.01) in the thermal nociceptive threshold in the diabetes group. Chronic nitrate or insulin treatment led to a significant decrease (p<0.01) in blood glucose levels, as well as a significant (p<0.05) increase in the body weight and serum NOx. Moreover, nitrate treatment significantly increased serum insulin levels (p<0.001) compared to the other groups.Conclusion. Chronic nitrate treatment modified the thermal and mechanical sensitivities in diabetic animals.

Sodium nitrate preconditioning prevents progression of the neuropathic pain in streptozotocin-induced diabetes Wistar rats

“…Insulin may directly supply strong support of neurons via its effects on brains insulin receptors or may indirectly by its beneficial effects on glycemia [66]. The results are in agreement with our previous findings that showed nitrate supplementation after 1 month of STZ-induced diabetes confirmation improved peripheral neuropathy [30].…”

“…All three behavioral tests were performed in a quiet room between 8:00 a.m. and 2:00 p.m. The von Frey and tail immersion tests were performed with an interval of 1-2 h [30]. All behavioral tests were performed in a blinded fashion.…”