Evaluation of Ambient Ozone Effect in Bean and Petunia at Two Different Sites under Natural Conditions: Impact on Antioxidant Enzymes and Stress Injury

Abstract

Tropospheric ozone is a harmful air pollutant and greenhouse gas that adversely affects living organisms. The effect of long-term ozone stress on the activity of SOD, APX, and GuPX, as well as lipid peroxidation and membrane injury in bean and petunia growing at a city site and in a forest, characterised by different ozone concentrations, was examined. The experiments were conducted in three growing seasons with different tropospheric ozone concentrations and meteorological conditions. Plants’ exposition to increased ozone concentration resulted in enhanced activity of antioxidant enzymes, level of lipid peroxidation, and membrane injury. In all years, higher ozone levels and solar radiation were observed at the forest site. The pattern of the changes in enzyme activity was dependent on ozone concentrations as well as on environmental conditions and varied from year to year. In the second year with the highest ozone concentration, the activity of GuPX and SOD increased the most. However, despite higher ozone concentration in the forest, a larger increase in APX and SOD activity in both species and GuPX activity in bean was recorded at the city site. The present results revealed that plant response to ozone might vary in different locations not only due to differences in ozone concentration but also because of the impact of other environmental factors, such as solar radiation and temperature.

Crop Resistance Mechanisms to Alleviate Climate Change-Related Stress

“…Salicylic acid is essential for maintaining antioxidant defense mechanisms and cellular redox responses in plants upon O 3 exposure (Hasan et al, 2021). Ozone-induced leaf damage accompanying ET biosynthesis has been confirmed by the inhibition of ET biosynthesis in tobacco following O 3 treatment (Bandurska et al, 2009). This suggests that the suppression of ET biosynthesis can increase O 3 tolerance in plants.…”