Evaluation of alternative strategies for generating fermentable sugars from high-solids alkali pretreated sugarcane bagasse and successive valorization to L (+) lactic acid

Nalawade, Ketaki; Baral, Pratibha; Patil, S S; Pundir, Anushka; Kurmi, Akhilesh Kumar; Konde, Kakasaheb; Patil, Sanjay; Agrawal, Deepti

doi:10.1016/j.renene.2020.05.089

Cited by 23 publications

(22 citation statements)

References 45 publications

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“…The study targeted the release of a minimum 10% glucose at high productivity from its cellulosic fraction using Cellic CTec2, a known new generation commercial cellulase (Cannella and Jørgensen., 2013). In our earlier study, substrate loading beyond 12.5% drastically reduced the glucose productivity and led to incomplete cellulose hydrolysis during shorter incubation time (Nalawade et al, 2020). Therefore to overcome the drawback of batch hydrolysis arising due to poor mixing, in the present study a two-stage (9% + 9%) substrate feeding strategy was adopted within a gap of one hour.…”

Section: Introductionmentioning

confidence: 94%

“…This concentrated sugar-rich solution was termed as "filtrate". Washing step was found inevitable to recover the adhered sugars from the biomass in our earlier study (Nalawade et al, 2020. Therefore, the residual biomass was water washed (equivalent to 50% filtrate volume) and said sugar solution was termed as "wash".…”

Section: Hses Of Alkali Pretreated Scbmentioning

confidence: 99%

“…Alkali pretreatment was conducted as per the method described previously with slight modifications (Nalawade et al, 2020). The previous study revealed when the water bath was set at 80C the difference between the water bath and the slurry temperature was ~15C.…”

Section: Alkali Pretreatment and Compositional Analysismentioning

confidence: 99%

“…Saccharification study was carried out at 18% (w/w) substrate loading in a non-buffered medium (pH 4.5) containing PEG 6000 (0.2g/g AISL content) placed in an incubator shaker set at 50C, 180 rpm as described previously (Baral et al, 2020). A two-step substrate feeding strategy (9% + 9%) within a gap of one hour was adopted to mitigate the problem of poor mixing as observed previously (Nalawade et al, 2020).…”

Section: Hses Of Alkali Pretreated Scbmentioning

confidence: 99%

“…The glucose-rich filtrates obtained after hydrolysis were valorized LA using a thermophilic bacterium B. coagulans NCIM 5648. The pre-seed and seed cultures were prepared in the modified CM5 medium with wash samples as a carbon source (Nalawade et al, 2020).…”

Section: Inoculum Preparation and La Fermentation By B Coagulans Ncim 5648mentioning

confidence: 99%

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Expeditious production of concentrated glucose-rich hydrolysate from sugarcane bagasse and its fermentation to lactic acid with high productivity

Baral

Pundir

Kumar

et al. 2020

Food and Bioproducts Processing

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Sugarcane bagasse (SCB) is anticipated to emerge as a potential threat to waste management in India on account of cheap surplus energy options and lower incentives through its cogeneration. Through biotechnological intervention, the efficient utilization of SCB is seen as an opportunity. The present study aimed towards expeditious production of concentrated glucose-rich hydrolysate from SCB. Alkali pretreated biomass was chosen for hydrolysis with a new generation cellulase cocktail, Cellic CTec2 dosed at 25mg g -1 glucan content. A two-step (9% + 9%) substrate feeding strategy was adopted with a gap of an hour, and saccharification was terminated in three different ways. Irrespective of the methods employed for termination, ~84.5% cellulose was hydrolyzed releasing ≥100 g L -1 glucose from 18% biomass. Direct use of glucose-rich filtrates yielded 69.2 ± 2.5 g L -1 of L (+) lactic acid (LA) using thermophilic Bacillus coagulans NCIM 5648. The best-attained glucose and LA productivities during separate hydrolysis and fermentation (SHF) in the present study were 5.27 and 2.88 g L -1 h -1 , respectively. A green and sustainable process is demonstrated for the production of industrially relevant sugars from SCB at high productivity and its valorization to bio-based LA.

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Section: Introductionmentioning

confidence: 94%

Section: Hses Of Alkali Pretreated Scbmentioning

confidence: 99%

Section: Alkali Pretreatment and Compositional Analysismentioning

confidence: 99%

Section: Hses Of Alkali Pretreated Scbmentioning

confidence: 99%

Section: Inoculum Preparation and La Fermentation By B Coagulans Ncim 5648mentioning

confidence: 99%

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Expeditious production of concentrated glucose-rich hydrolysate from sugarcane bagasse and its fermentation to lactic acid with high productivity

Baral

Pundir

Kumar

et al. 2020

Food and Bioproducts Processing

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Cost reduction approaches for fermentable sugar production from sugarcane bagasse and its impact on techno-economics and the environment

et al. 2021

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In an enzymatically driven lignocellulosic biorefinery, pretreatment and hydrolysis modules are the two most significant cost contributors for obtaining high gravity sugar solutions. The present study aimed to reduce the use of alkali and Cellic CTec2 during the bioprocessing of sugarcane bagasse (SCB). Later its impact on the overall process economics and the environment was evaluated. During pretreatment, solid loading of 15% (w/w) and use of 2% (w/v) sodium hydroxide at 121C for 30 min emerged as an optimum strategy. It resulted in >65% delignification of SCB, retaining ≥ 90 % and 65 % of glucan and xylan fraction, respectively, in the pretreated biomass. Two approaches were evaluated in parallel to minimize the requirement of this commercial cellulase enzyme blend. The first strategy involved its partial replacement with an in-house enzyme cocktail by blending. The second route was performing hydrolysis with reduced loadings of cellulase enzyme blend above its optimum temperature, which gave more promising results. Hydrolysis of 20% alkali pretreated SCB with cellulase enzyme blend dosed at 15 mg protein g-1 glucan led to 84.13 ± 1 and 83.5 ± 2.3% glucan and xylan conversion yields respectively in 48h at 52.5C. The filtrate and wash fraction contained ≥ 165 and ≥ 65 g L-1 sugar monomers representing glucose and xylose. However, in both the fractions >75%, sugar accounted for glucose. The techno-economic analysis revealed that the sugar production cost from SCB was 1.32 US$/kg, with the optimized bioprocess. Environmental impact study showed that the process contributed to 1.57 kg CO2 eq in terms of climate change.

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Assessment of multiple pretreatment strategies for 2G L-lactic acid production from sugarcane bagasse

Nalawade

Saikia

Behera

et al. 2020

Biomass Conv. Bioref.

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Evaluation of alternative strategies for generating fermentable sugars from high-solids alkali pretreated sugarcane bagasse and successive valorization to L (+) lactic acid

Cited by 23 publications

References 45 publications

Expeditious production of concentrated glucose-rich hydrolysate from sugarcane bagasse and its fermentation to lactic acid with high productivity

Expeditious production of concentrated glucose-rich hydrolysate from sugarcane bagasse and its fermentation to lactic acid with high productivity

Cost reduction approaches for fermentable sugar production from sugarcane bagasse and its impact on techno-economics and the environment

Assessment of multiple pretreatment strategies for 2G L-lactic acid production from sugarcane bagasse

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