Evaluation of allogeneic freeze-dried platelet lysate in cartilage exposed to interleukin 1-β in vitro

Abstract

BackgroundPlatelet-rich plasma (PRP) as well as other platelet-derived products have been used as a potential disease-modifying treatment for musculoskeletal diseases, such as osteoarthritis (OA). The restorative properties of such products rely mainly on the high concentrations of growth factors, demonstrating encouraging results experimentally and clinically. Yet, the autologous blood-derived nature of the PRP product lead to limitations that precludes it’s widespread use. The main limitations for PRP use are; product variability, the need for minimum laboratory settings in most cases, and the need for storage at low temperatures to preserve its properties. Based on these limitations, the objective of this study was to investigate an allogeneic off-the-shelf platelet lysate (PL) in cartilage exposed to interleukin 1β (IL-1β). For this purpose, blood and cartilage were harvested from eight skeletally mature and healthy horses. Blood was processed into PL aliquots and divided into three groups (Frozen, Freeze-dried and Filtered freeze-dried), used in autologous and allogeneic conditions and in three different concentrations (1.5, 3 and 6-fold). Different PL preparations were then applied in cartilage culture with interleukin-1 beta and cultured for 10 days. Cartilage and media samples were collected and analyzed for total GAG and 35SO4-labeled GAG content.ResultsNo significant differences between the controls and PL groups in cartilage and media were demonstrated. The effects of PL on cartilage matrix were concentration dependent and intermediate concentrations (3-fold) in PL showed increased 35SO4-labelled GAG in cartilage.ConclusionIn conclusion, the allogeneic freeze-dried PL presented equivalent effects compared to frozen autologous PL. Intermediate platelet concentration on average demonstrated improved results, demonstrating less GAG loss compared to other concentrations.

Comparison of Allogeneic Platelet-rich Plasma With Autologous Platelet-rich Plasma for the Treatment of Diabetic Lower Extremity Ulcers

“…This is the first study to confirm that al-PRP has an equivalent effectiveness and safety to accelerate wound healing of DLEUs. Furthermore, the present study was supported by a basic study of cartilage culture, in which the results suggested that allogeneic freeze-dried platelet lysate presented equivalent effects compared to frozen autologous platelet lysate 50 .…”

Retracted: An evaluation of allogeneic freeze-dried concentrated growth factors biocompatibility in vitro and in vivo

“…Finally, the allogeneic CGF could be used in patients in whom CGF cannot be extracted due to medical conditions. Some studies have reported that freeze-dried platelets and platelet lysate can be used for cell culture and tissue healing [12,13]. CGF is a concentrate of blood platelets, and freeze-drying could be used for CGFs, which could expand the scope of use of CGF for healing applications.…”

Freeze-Drying of Platelet-Rich Plasma: The Quest for Standardization

“…The latter was achieved through a reduction in common inflammatory molecular pathways, such as NF-kB activation and nitric oxide (NO) synthesis. Similarly, dose-related chondroprotective actions of PRP, without differences between FD-PRP compared to fresh-frozen PRP, were found in equine cartilage cultures [51]. In a similar way, standardized human PRP powder induced a dose-and time-response relationship in human chondrocyte proliferation and metabolic activity [52].…”