Evaluation of Alginate and Hyaluronic Acid for Their Use in Bone Tissue Engineering

Rubert, Marina; Alonso-Sande, M.; Monjo, Marta; Ramis, Joana Maria

doi:10.1007/s13758-012-0044-8

Cited by 33 publications

(17 citation statements)

References 43 publications

(63 reference statements)

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“…Degradation properties of alginate might be due to an outward flux of calcium ions into the surrounding medium, thus decreasing crosslinking overtime. In this study, no differences on cell viability were observed between coated and uncoated TiO 2 SC, in agreement with previous reports showing cell safety and biocompatibility of TiO 2 SC7 and of alginate gels 19, 26–28. Alginate has been widely used for delivery of bioactive molecules27, 29 and constitute a suitable carrier for delivery of synthetic peptides at the local site 19.…”

Section: Discussionsupporting

confidence: 92%

“…Alginate has been widely used for delivery of bioactive molecules27, 29 and constitute a suitable carrier for delivery of synthetic peptides at the local site 19. To avoid serum proteins or phenol interference from the cell culture media, peptide release was performed in distilled water in the present study, although in an early study,28 P2 labeled with fluorescein isothiocyanate (FITC) was used and measured in the cell culture media showing a similar release profile. Thus, we found a burst release of P2 during the first hours of incubation followed by progressive and sustained release during the 21‐days period.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Rates of hospitalized bacterial infection associated with juvenile idiopathic arthritis and its treatment

Beukelman

Xie

Chen

et al. 2012

Arthritis & Rheumatism

157

126

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Objective Compare the incidence of hospitalized bacterial infections among children with and without juvenile idiopathic arthritis (JIA) and examine the effects of selected medications Methods Using national U.S. Medicaid data from 2000–2005, we identified a JIA cohort and a comparator cohort of children with attention-deficit hyperactivity disorder (ADHD). Exposures to methotrexate (MTX), TNF inhibitors, and oral glucocorticoids were determined using pharmacy claims. Hospitalized bacterial infections were identified using coded discharge diagnoses. We calculated adjusted hazard ratios (aHR) to compare infection incidence rates while adjusting for relevant covariates. Results We identified 8,479 JIA patients with 13,003 person-years of follow-up; 42% used MTX and 17% used TNF inhibitors. Compared with ADHD patients, JIA patients without current MTX or TNF inhibitor use had an increased rate of infection (aHR 2.0; 95%CI 1.5–2.5). Among JIA patients not using TNF inhibitor therapy, MTX users had a similar rate of infection compared with those without current MTX use (aHR 1.2; 95%CI 0.9–1.7). TNF inhibitor use (irrespective of MTX) resulted in a similar rate of infection compared to MTX without TNF inhibitor (aHR 1.2; 95%CI 0.8–1.8). With adjustment for MTX and TNF inhibitor use, high-dose glucocorticoid use (≥10 mg of prednisone daily) increased the rate of infection compared with no glucocorticoid use (aHR 3.1; 95%CI 2.0–4.7). Conclusions Children with JIA had an increased rate of infection compared to children with ADHD. Among children with JIA, the rate of infection was not increased with MTX or TNF inhibitor use, but was significantly increased with high-dose glucocorticoid use.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Rates of hospitalized bacterial infection associated with juvenile idiopathic arthritis and its treatment

Beukelman

Xie

Chen

et al. 2012

Arthritis & Rheumatism

157

126

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“…Durometry yields precise data about tissue firmness and can detect subtle differences in skin texture. [20][21][22][23][24][25] In this study, durometry revealed that skin injected with 3D MA-HA had similar firmness as the control groups and no changes over the course of 30 days were noted (Fig. 6).…”

Section: Discussionsupporting

confidence: 50%

Injectable Shape-Memorizing Three-Dimensional Hyaluronic Acid Cryogels for Skin Sculpting and Soft Tissue Reconstruction

Cheng

Shih

et al. 2017

Tissue Engineering Part A

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Introduction: Hyaluronic acid (HA)-based fillers are used for various cosmetic procedures. However, due to filler migration and degradation, reinjections of the fillers are often required. Methacrylated HA (MA-HA) can be made into injectable shape-memorizing fillers (three-dimensional [3D] MA-HA) aimed to address these issues. In this study, shape retention, firmness, and biocompatibility of 3D MA-HA injected subcutaneously in mice were evaluated. Materials and Methods: Fifteen mice, each receiving two subcutaneous injections in their back, were divided into four groups receiving HA, MA-HA, 3D MA-HA, or saline, respectively. Digital imaging, scanning electron microscope (SEM) and in vivo imaging system (IVIS), durometry, and histology were utilized to evaluate in vitro/ vivo degradation and migration, material firmness, and the angiogenic (CD31) and immunogenic (CD45) response of the host tissue toward the injected materials. Results: Digital imaging, SEM, and IVIS revealed that 3D MA-HA fillers maintained their predetermined shape for at least 30 days in vitro and in vivo. Little volume effects were noted in the saline and other control groups. There were no differences in skin firmness between the groups or over time. Histology showed intact skin architecture in all groups. Three-dimensional MA-HA maintained its macroporous structure with significant angiogenesis at the 3D MA-HA/skin interfaces and throughout the 3D MA-HA. There was no significant inflammatory response to any of the injected materials. Conclusion: 3D MA-HA showed remarkable tissue compatibility, compliance, and shape predictability, as well as retention, and thus might be suitable for various skin sculpting and soft tissue reconstruction purposes.

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“…Since HA is a primary ECM component, it has been investigated as scaffolding material for soft tissue engineering applications [1][2][3][4]. HA is biocompatible and a key component in bone and cartilage tissue engineering applications, and thus, a wide range of additional biomedical applications have also been explored [5,6].…”

Section: Introductionmentioning

confidence: 99%

Phosphate salts facilitate the electrospinning of hyaluronic acid fiber mats

et al. 2013

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Electrospinning is a cost effective and facile method to manufacture fiber mats appropriate for biomedical applications. Due to its high molecular weight and charged backbone, hyaluronic acid (HA) fiber mats with consistent fiber morphology have been difficult to electrospin from neutral pH solutions. Here, we present that the electrospinning of HA fibers in aqueous dimethylformamide solutions is facilitated by the addition of three phosphate salts. The salts-glycerol phosphate (GP), sodium phosphate (SP), and tripolyphosphate (TPP)-facilitated electrospinning of the solutions as characterized by conductivity measurements and fiber morphology. From tensile experiments, HA mats electrospun with SP demonstrated improved Young's modulus (12 MPa) over HA mats spun with either GP or TPP (5 and 3 MPa, respectively). This work demonstrates that a new neutral solvent system can be employed to spin HA fibers, which offers the potential for using the fibers for biomedical applications, such as a bone biomimetic.

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Evaluation of Alginate and Hyaluronic Acid for Their Use in Bone Tissue Engineering

Cited by 33 publications

References 43 publications

Rates of hospitalized bacterial infection associated with juvenile idiopathic arthritis and its treatment

Rates of hospitalized bacterial infection associated with juvenile idiopathic arthritis and its treatment

Injectable Shape-Memorizing Three-Dimensional Hyaluronic Acid Cryogels for Skin Sculpting and Soft Tissue Reconstruction

Phosphate salts facilitate the electrospinning of hyaluronic acid fiber mats

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