Evaluation of agro-food byproducts for gluconic acid production by Aspergillus niger ORS-4.410

Singh, Om V.; Kapur, Nidhi; Singh, Rajesh

doi:10.1007/s11274-004-2395-x

Cited by 30 publications

(24 citation statements)

References 13 publications

(15 reference statements)

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“…This data was in accordance with (Singh, Kapur, & Singh, 2005) whereas they indicated that there is an abundant growth of A. niger was observed with crude grape and banana-must. So they reported that grape-must and banana-must were utilized as the sole sources for gluconic acid production.…”

Section: 6supporting

confidence: 89%

Production of gluconic acid by using some irradiated microorganisms

Ahmed

Farag

Hassan

et al. 2015

Journal of Radiation Research and Applied Sciences

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a b s t r a c tThe objective of this study was to isolate the potential fungal isolates have the ability for gluconic acid production by using some agro industrial byproducts as sugarcane molasses, banana-must and grape-must. The effect of gamma-irradiation on the most potent isolates and the fermentation conditions as pH, incubation temperature and incubation period was also investigated. Results showed that the most potential fungal isolates were Aspergillus niger, Penicillium puberulum and Penicillium frequentans whereas their gluconic acid production was 62.17, 56.25 and 39.69 g/L, respectively on Czapek's Dox media at 28 ± 1 C, pH 6 for 7 days fermentation period. Irradiation of the three most potential isolates at 0.1, 0.2, 0.3, 0.4 and 0.5 kGy doses of gamma ray showed that 0.1 kGy dose caused an increase in gluconic acid production whereas it was 69.35, 60.17 and 40.31 g/L by the three potential isolates respectively. Data showed that utilization of sugarcane molasses, banana-must and grape-must as a sole carbon source in gluconic acid production by the three potential (0.1 kGy) irradiated isolates at pH 6, 30 C for a 7 days incubation period caused increasing in gluconic acid production whereas the productivity of the three (0.1 kGy) irradiated isolates (A. niger, P. puberulum and P. frequentans) was 69.87, 63.14 and 51.28 g/L by utilizing sugarcane molasses, 61.28, 56.37, 47.15 g/L by utilizing banana-must and 54.25, 52.75 and 44.75 g/L by utilizing grape-must.

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Section: 6supporting

confidence: 89%

Production of gluconic acid by using some irradiated microorganisms

Ahmed

Farag

Hassan

et al. 2015

Journal of Radiation Research and Applied Sciences

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“…No acids were determined in case of Trichoderma harzianum Rifai 1295-22 in an HPTLC analysis with diethyl ether: formic acid: water as mobile phase (Altomare et al 1999). Singh et al (2005) detected the concentration of gluconic acid produced by Aspergillus niger in different carbon sources containing media with the help of HPLC with acetonitrile: water as mobile phase. The production of various organic acids in the culture solution is considered the main mechanism for the solubilization of insoluble phosphate (Cunningham and Kuiack 1992).…”

Section: Discussionmentioning

confidence: 99%

“…Then it was developed with diethyl ether: formic acid: water (70:20:10) (Altomare et al 1999). Another set of the loaded samples and standards were developed in acetonitrile: water (70:30) (Singh et al 2005). After 20-30 min of activation, the TLC sheets were allowed to dry for 1 h and scanned in TLC Scanner (Linomat 5, Camag TLC scanner-3) with speed of 100 mm/s and data resolution 100 lm/step.…”

Section: Hptlc Analysis Of Organic Acidsmentioning

confidence: 99%

Slow and steady phosphate solubilization by a psychrotolerant strain of Paecilomyces hepiali (MTCC 9621)

Rinu

Pandey

2010

World J Microbiol Biotechnol

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A psychrotolerant phosphate solubilizing fungus has been isolated from the rock soil of a cold desert site in Indian Himalaya. The fungus grows from 4 to 35°C (optimum 21°C), and from 2 to 13.5 pH (optimum 9) under laboratory conditions. Based on phenotypic characters and 26S rDNA analysis, the fungus is identified as Paecilomyces hepiali. In quantitative estimation that was carried out at 9, 14, and 21°C, the fungus solubilized maximum phosphate at 14°C. In view of the slow growth and persistence of the desired activity at low temperature, the estimation was carried out for a longer period, i.e., up to 6 weeks. The suboptimal conditions for growth and biomass production were found to be optimal for phosphate solubilization by the fungus. At 14 and 9°C, the solubilization touched its maximum on day 42. Decline in pH was found to be significantly correlated with the phosphate solubilization at all the temperatures, under consideration. The acid phosphatase activity was found to be more prominent than alkaline phosphatase in culture filtrate. High performance thin layer chromatography (HPTLC) analysis showed production of six organic acids, gluconic and a-keto glutaric acid being in maximum amount in the culture filtrate. The study has ecological significance in view of the nutrient cycling under low temperature environment, prevalent in Himalayan region.

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“…Sing et al [15] reported GA production by Aspergillus niger ORS-4.410 using rectified grape must, banana must and treated molasses must under submerged fermentation, it showed GA production 73.2 gL -1 , 69.3 gL -1 and 58.32 gL -1 with product formation rate 0.509, 0.481 and 0.405 respectively [14]. Sapkal and Kulkarni (2002) reported GA production in a continuous recirculation reactor using immobilized A. niger on cellulose microfibers and obtained 158 g/l GA with 0.28 gL -1 h -1 production rate [25].…”

Section: Resultsmentioning

confidence: 99%

Gluconic Acid Production from Golden Syrup by Aspergillus niger Strain using Semiautomatic Stirred-Tank Fermenter

Purane¹

2012

J Microb Biochem Technol

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The gluconic acid batch fermentation was conducted using mutant Aspergillus niger NCIM 530 strain under submerged condition in 50 L semiautomatic stirred-tank fermenter. Certain cost-effective source as golden syrup was effectively utilized instead of glucose for successful industrial fermentation process. The significant level of gluconic acid (85.2 gL -1 ) production was observed with maximum 86.97% glucose conversion over 44 hours. This process provides great advantages over traditional submerged fermentation strategies and substrates, as showed by effective production of gluconic acid by utilizing novel substrate as a golden syrup. To reduce analysis time with better accuracy, an effort has been made to use a method for evaluation of parameters like conversion of substrate and production of gluconic acid during the fermentation by using High Performance Thin Layer Chromatography (HPTLC).

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Evaluation of agro-food byproducts for gluconic acid production by Aspergillus niger ORS-4.410

Cited by 30 publications

References 13 publications

Production of gluconic acid by using some irradiated microorganisms

Production of gluconic acid by using some irradiated microorganisms

Slow and steady phosphate solubilization by a psychrotolerant strain of Paecilomyces hepiali (MTCC 9621)

Gluconic Acid Production from Golden Syrup by Aspergillus niger Strain using Semiautomatic Stirred-Tank Fermenter

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