Evaluation of agricultural byproducts for the production of betaglucosidase by Aspergillus niger MBT-2 using solid state fermentation

Abstract

The experiment was conducted to isolate and screen fungal strain and optimization of solid-state fermentation conditions for enhanced production of β-glucosidase. Different fungal cultures were isolated and screened for β-glucosidase production. The physicochemical and nutritional parameters were optimized for enhanced production of β-glucosidase from higher producer. Among all the isolates the isolate which exhibited highest β-glucosidase potential was identified and assigned the code as Aspergillus niger MBT-2. The optimum β-glucosidase production was obtained in M5 medium containing wheat bran after 72 hrs of incubation at 40°C, pH 6 and 20 ml of moisture contents. In addition to this 2% fructose and 2% yeast extract proved to be best carbon and nitrogen sources, respectively and gave maximal enzyme productivity. The exploitation of agricultural by products as a substrate reduced the production cost of enzyme and makes the process economical. The Aspergillus niger MBT-2 has promising potential of bioconversion of low-cost material into valuable product like β-glucosidase.

Optimisation of β-Glucosidase Production in a Crude Aspergillus japonicus VIT-SB1 Cellulase Cocktail Using One Variable at a Time and Statistical Methods and its Application in Cellulose Hydrolysis

“…Wheat bran has been reported to be the carbon source of choice as it is not only a good inducer of cellulases and hemicellulases, but is also cheap. The complex structure of wheat bran makes it difficult for filamentous fungi to penetrate the structure, and as a result it produces more enzymes to aid penetration [ 20 ]. Vaithanomsat et al [ 21 ] and Abdullah et al [ 20 ] reported optimal β -glucosidase production by A. niger and A. oryzae , respectively, on wheat bran.…”

“…The complex structure of wheat bran makes it difficult for filamentous fungi to penetrate the structure, and as a result it produces more enzymes to aid penetration [20]. Vaithanomsat et al [21] and Abdullah et al [20] reported optimal β-glucosidase production by A. niger and A. oryzae, respectively, on wheat bran.…”

“…Optimum pH for enzyme production was determined by inoculation of minimal salt medium with different pH buffers: 50 mM sodium acetate buffer (pH 3.0 to 5.0), 50 mM sodium phosphate buffer (pH 6.0 to 8.0), and 50 mM glycine-NaOH buffer (pH 9.0-10.0) were incubated for their optimal time for production and thereafter assayed for enzyme activity. The optimum incubation temperature was determined by setting up shake flask cultures with media at the optimal pH and incubating them at different temperatures (20,30,40,50,60,70, and 80 • C) at 125 rpm for the optimal time duration. Thereafter, the extracts were assayed for enzyme activity.…”