Evaluation of aglianico grape skin and seed polyphenol astringency by SDS–PAGE electrophoresis of salivary proteins after the binding reaction

Gambuti, Angelita; Rinaldi, Alessandra; Pessina, R.; Moio, Luigi

doi:10.1016/j.foodchem.2005.05.038

Cited by 86 publications

(66 citation statements)

References 46 publications

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“…In contrast, CAM fHZ berries showed a lower content of total anthocyanins extracted from skins and a similar content of total polyphenols and tannins extracted from seeds. Taking into account the facts that (i) anthocyanins are mainly extracted during the first phases of red vinification (consisting in the maceration of whole berries during must fermentation), (ii) complete extraction from the seeds requires the berry skins and seeds to be in contact for a longer time with must-wine, and (iii) seed tannins are more astringent than skin tannins (Gambuti et al, 2006), these data suggest that a specific winemaking procedure, such as short maceration, could help obtain a red wine from CAM fHZ grapes with a good colour intensity which is not astringent and which is easier to drink.…”

Section: Discussionmentioning

confidence: 99%

Functional homogeneous zones (fHZs) in viticultural zoning procedure: an Italian case study on Aglianico vine

Bonfante

Agrillo

Albrizio

et al. 2015

SOIL

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Abstract. This paper aims to test a new physically oriented approach to viticulture zoning at farm scale that is strongly rooted in hydropedology and aims to achieve a better use of environmental features with respect to plant requirements and wine production. The physics of our approach are defined by the use of soil-plant-atmosphere simulation models, applying physically based equations to describe the soil hydrological processes and solve soil-plant water status.This study (part of the ZOVISA project) was conducted on a farm devoted to production of high-quality wines (Aglianico DOC), located in southern Italy (Campania region, Mirabella Eclano, AV). The soil spatial distribution was obtained after standard soil survey informed by geophysical survey. Two homogeneous zones (HZs) were identified; in each one a physically based model was applied to solve the soil water balance and estimate the soil functional behaviour (crop water stress index, CWSI) defining the functional homogeneous zones (fHZs). For the second process, experimental plots were established and monitored for investigating soil-plant water status, crop development (biometric and physiological parameters) and daily climate variables (temperature, solar radiation, rainfall, wind).The effects of crop water status on crop response over must and wine quality were then evaluated in the fHZs. This was performed by comparing crop water stress with (i) crop physiological measurement (leaf gas exchange, chlorophyll a fluorescence, leaf water potential, chlorophyll content, leaf area index (LAI) measurement), (ii) grape bunches measurements (berry weight, sugar content, titratable acidity, etc.) and (iii) wine quality (aromatic response). This experiment proved the usefulness of the physically based approach, also in the case of mapping viticulture microzoning.

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Section: Discussionmentioning

confidence: 99%

Functional homogeneous zones (fHZs) in viticultural zoning procedure: an Italian case study on Aglianico vine

Bonfante

Agrillo

Albrizio

et al. 2015

SOIL

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“…That may reflect changes due to seed maturation, development of the seed coat, and differences in rate of polymerization attributed to flavanol galloylation, demonstrated elsewhere in model solutions (Cheynier and Ricardo-da-Silva 1991). Although research with purified PAs suggests that those originating from seed will affect astringency and may be coarse or bitter, application to wine production and quality remains uncertain (Gambuti et al 2006). Seed additions during wine production have resulted in improvements in wine color and potential aroma character .…”

Section: Discussionmentioning

confidence: 99%

Diurnal Temperature Range Compression Hastens Berry Development and Modifies Flavonoid Partitioning in Grapes

Cohen¹,

Tarara²,

Kennedy

2011

Am. J. Enol. Vitic.

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Abstract:Temperatures during the day and night are known to influence grape berry metabolism and resulting composition. In this study, the flavonoid composition of field-grown Vitis vinifera L. cv. Merlot berries was investigated as a function of diurnal temperature range (DTR). The DTR was compressed by cooling berries during the day and heating them at night. Before veraison, there were minor differences in proanthocyanidin (PA) composition in the skins and seeds due to temperature treatments, most notably a decrease in gallate-esterification of seed flavan-3-ols with compressed DTR. Compressing the DTR significantly hastened berry development and the inception of veraison. Treatments imposed after veraison had minimal impact on skin and seed PAs; however, compressed DTRs favored the partitioning of anthocyanins and flavonols toward B-ring di-substitution. Compressing the DTR of grape berries had a consistent effect on berry development and partitioning of flavonoid metabolites while total flavonoid content was not significantly altered.

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“…These proteins are the main responsible molecules for the viscoelastic properties of saliva. Some authors reported the binding of mucins to polyphenols, and consequently an effect on astringency development [54]. On the other hand, other authors [e.g.…”

Section: Mechanical Sensations (Astringency)mentioning

confidence: 99%

“…Dinella et al [88,89], by separating proteins through SDS-PAGE, presented evidences that differences among individuals in sensitivity for astringent stimuli relates to the differences they present in salivary protein composition after repeated stimulation, i.e., individuals in which tannin stimulation results in higher changes in salivary protein profiles are the ones who strongly detect astringency. The evaluation of polyphenol astringency was also demonstrated to be possible by using SDS PAGE to separate the salivary proteins present in human saliva after mixture with these plant secondary metabolites [54].…”

Section: The Use Of Sds Page and 2-de In Salivary Proteome Study Relamentioning

confidence: 99%

The Use of Electrophoresis for the Study of Saliva Involvement in Ingestive Behavior

Lamy¹,

Morzel²,

Rodrigues³

et al. 2015

Field Effect Electroosmosis - A Novel Phenomenon in Electrokinetics and Its Applications in Capillary Electrophoresis

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Evaluation of aglianico grape skin and seed polyphenol astringency by SDS–PAGE electrophoresis of salivary proteins after the binding reaction

Cited by 86 publications

References 46 publications

Functional homogeneous zones (fHZs) in viticultural zoning procedure: an Italian case study on Aglianico vine

Functional homogeneous zones (fHZs) in viticultural zoning procedure: an Italian case study on Aglianico vine

Diurnal Temperature Range Compression Hastens Berry Development and Modifies Flavonoid Partitioning in Grapes

The Use of Electrophoresis for the Study of Saliva Involvement in Ingestive Behavior

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