Evaluation of African medicinal plants for their in vitro trypanocidal activity

Freiburghaus, F; Kaminsky, Ronald; Nkunya, Mayunga H.H.; Brun, Reto

doi:10.1016/s0378-8741(96)01463-8

Cited by 174 publications

(111 citation statements)

References 18 publications

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“…Our results support those of Freiburghaus et al (1996) who showed that leaf extracts of S. spinosa and H. acida were active in vitro on T. b. rhodesiense. The activity of S. spinosa leaf extracts were close to those observed in our study, while the range of MIC values were quite different for H. acida leaf extracts.…”

Section: Discussionsupporting

confidence: 92%

“…We also noted variations in the antitrypanosomal activity of the candidate plants depending on the time and place of collection (data not shown). Freiburghaus et al (1996) tested rootbark extracts of S. spinosa as well as root extracts of H. acida which were also active in vitro and analysed the selectivity of these root extracts on human fibroblasts. In our studies we focused on the leaves which are the plant part used in traditional medicine and we examined the effects of these leaf extracts on another protozoan parasite (L. m. mexicana) and on mammalian cells.…”

Section: Discussionmentioning

confidence: 99%

“…In our studies we focused on the leaves which are the plant part used in traditional medicine and we examined the effects of these leaf extracts on another protozoan parasite (L. m. mexicana) and on mammalian cells. Freiburghaus et al (1996) also investigated the activity of leaf extracts of T. roka, which is now named T. emetica subsp. emetica (White, 1986), which were inactive.…”

Section: Discussionmentioning

confidence: 99%

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In vitro antitrypanosomal activity of ethnopharmacologically selected Beninese plants

Hoet

Opperdoes

Brun

et al. 2004

Journal of Ethnopharmacology

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The in vitro antitrypanosomal activity of methylene chloride, methanol and aqueous extracts of the leaves and twigs of five plant species traditionally used in Benin for the treatment of sleeping sickness were evaluated on Trypanosoma brucei brucei and their selectivity was analysed on Leishmania mexicana mexicana and J774 macrophage-like murine cells. The results showed that the four most active extracts had MIC values ≤19 g/ml (Hymenocardia acida twig and leaf, Strychnos spinosa leaf, Trichilia emetica leaf methylene chloride extracts).All these extracts had a lower activity on L. m. mexicana and J774 cells. Determination of the IC 50 values of the methylene chloride leaf extracts on two strains of trypanosomes (T. b. brucei and T. b. rhodesiense) and two mammalian cell lines (L6 and J774 cells) showed that all extracts possessed some antitrypanosomal activity with IC 50 's ranging from 1.5 to 39 g/ml. All were also toxic to the mammalian cells, but usually with higher IC 50 's. The only exception was the S. spinosa methylene chloride leaf extract which had no toxicity on J774 cells. Although tannins have been identified in most of the species studied, they could not be detected in the most active extracts, just as alkaloids. The presence of flavonoids and quinones may at least in part explain the observed activities of some of the active extracts.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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In vitro antitrypanosomal activity of ethnopharmacologically selected Beninese plants

Hoet

Opperdoes

Brun

et al. 2004

Journal of Ethnopharmacology

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“…All these underscore the need for new, effective and inexpensive drugs for the treatment of trypanosomosis. Plants have always been among the common sources of medicaments, either processed as traditional preparations, or used to prepare pure active principles (Freiburghaus et al, 1996). In Africa, herbal treatment has a long tradition and still holds a strong position in medical care.…”

Section: Introductionmentioning

confidence: 99%

Investigation of the Antitrypanosomal Activity of Buchholzia coriacea Seed Extract against a Field Strain of Trypanosoma congolense

Nweze

Anene

Asuzu

2011

Afr. J. Trad. Compl. Alt. Med.

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The antitrypanosomal activity of the methanol extract of Buchholzia coriacea seed against a field strain of Trypanosoma congolense was investigated using experimentally infected mice of both sexes. Monitoring of parasitaemia was by the rapid matching technique. When parasitaemia was approximately log 7.8 (63 x 10 6 parasites/ml), treatment with graded doses of the extract (250, 500 and 1000 mg/kg) was instituted for 5 consecutive days. Diminazene diaceturate (Dimivet ® SKM Pharma Pvt. Ltd.) was given at 3.5 mg/kg i.p. to the positive control mice. No significant differences in body weights were observed. The rectal temperatures of infected mice showed fluctuations. The PCV of infected mice were significantly (p < 0.05) lower than those of the uninfected controls. There was no significant difference between the PCV of the extract-treated and untreated animals. Parasitaemia increased steadily in the extract-treated and untreated mice groups till all the animals died. Three days post-treatment with diminazene diaceturate parasitaemia was cleared. Six days later, there was a relapse of infection. By the end of the experiment, a 50 % relapse rate was recorded in the diminazene diaceturatetreated group. The methanol extract of Buchholzia coriacea seeds did not show any antitrypanosomal activity in mice infected with Trypanosoma congolense at the doses tested.

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“…Efforts in the last two decades 8,[14][15][16][17][18] suggest that plants may provide the much needed clue for the emergence of the long awaited new generation of trypanocidal drugs. In line with this trend, we investigated different solvent extracts of different parts of Prosopis africana for in vitro and in vivo trypanocidal activity using Trypanosoma brucei brucei as model.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Anti-trypanosomal Properties of Four Extracts of Leaves, Stem and Root Barks of Prosopis africana in Laboratory Animals

Atawodi¹,

Ogunbusola²

2010

Biokemistri

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Qualitative phytochemical and anti-trypanosomal properties of the petroleum ether, chloroform, methanol and aqueous extracts, obtained by cold extraction from the leaves, stem bark and roots of Prosopis africana were evaluated. The methanolic and aqueous extracts of the stem bark and leaves of the plant contained alkaloids, saponins, tannins and flavonoids. Anthraquinone was present in the stem bark methanolic extract and in the methanolic and aqueous extracts of the root as well as the aqueous extract of the leaves. Resins was present in the petroleum ether and chloroform extracts of the stem bark and leaves, while tannins was detected in the methanol and aqueous extracts of the stem bark and leaves of the plant in addition to the petroleum ether and chloroform extracts of the root bark. All the solvent extracts showed strong in vitro anti-trypanosomal activity and 2 and 4 mg/ml, but in vivo only the methanolic extract of the leaves displayed the most promising anti-trypanosomal effect at 200 mg/kg dose. Hence, Prosopis africana extracts possess significant anti-trypanosomal activity to warrant bioassay-guided evaluation and identification of the active principle.

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Evaluation of African medicinal plants for their in vitro trypanocidal activity

Cited by 174 publications

References 18 publications

In vitro antitrypanosomal activity of ethnopharmacologically selected Beninese plants

In vitro antitrypanosomal activity of ethnopharmacologically selected Beninese plants

Investigation of the Antitrypanosomal Activity of <i>Buchholzia coriacea</i> Seed Extract against a Field Strain of <i>Trypanosoma congolense</i>

Evaluation of Anti-trypanosomal Properties of Four Extracts of Leaves, Stem and Root Barks of <i>Prosopis africana</i> in Laboratory Animals

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