Evaluation of aerobic and anaerobic methods for recovery of streptococci from throat cultures

Randolph, Martin F.; Redys, John J.; Cope, John B.

doi:10.1016/s0022-3476(84)80491-6

Cited by 19 publications

(2 citation statements)

References 6 publications

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“…Oh the other hand, an anaerobic atmosphere used for one or two days of incubation of either SXT • BA or SBA cultures has been found to provide significantly greater recovery of 5. pyogenes in numerous reports. 1,2,11,16,22,24,38 In the present study, no attempt was made to compare the efficiency of either SXT • BA or SBA incubated in each possible atmosphere because of the volume of such data already available. Selection of SXT • BA incubated aerobically (5-10% CO2) and SBA incubated anaerobically was made because, from a review of the literature, these conditions appeared to offer an optimal potential for recovery of group A streptococci.…”

Section: Discussionmentioning

confidence: 99%

Suitability of a Throat Culture Method for Evaluation of Group A Streptococcal Antigen Detection Kits

Kellogg¹,

Bankert²,

Levisky³

1986

American Journal of Clinical Pathology

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Previous reports have indicated a wide variation in observed sensitivity of antigen-detection kits for group A streptococci. Before undertaking an evaluation of these new kits, the sensitivity of the throat culture technic routinely used by this laboratory was reexamined. Each throat swab was directly inoculated to sheep blood agar containing trimethoprim-sulfamethoxazole (SXT X BA) and drug-free sheep blood agar (SBA) plates. Swabs were then washed in saline and the saline used to inoculate one more of each type of medium. SXT X BA cultures were incubated aerobically (5 to 10% CO2), and SBA cultures were incubated anaerobically, both for two days at 35 degrees C. From 726 patients, 164 (22.6%) of the specimens contained group A streptococci, 99% detected on directly inoculated cultures and 100% on cultures inoculated with the saline wash. Either an aerobically (CO2) incubated SXT X BA or an anaerobically incubated SBA, directly inoculated and held for two days, appears to offer a satisfactory reference culture method for the recovery of group A streptococci.

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Section: Discussionmentioning

confidence: 99%

Suitability of a Throat Culture Method for Evaluation of Group A Streptococcal Antigen Detection Kits

Kellogg¹,

Bankert²,

Levisky³

1986

American Journal of Clinical Pathology

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“…The manufacturer's product insert claims a TISA sensitivity of 93 % . Anaerobically-incubated sheep blood agar cultures were used as a means of comparison during the current study because this method has been shown to provide a recovery of the streptococcal pathogen ranging from 95 % to 100% in numerous studies (11)(12)(13)(14)(15)(16).…”

Section: Introductionmentioning

confidence: 99%

Performance of the tandem ICON strep A® enzyme immunoassay system for detection of group a streptococci from oropharyngeal swabs

Kellogg

Bankert

Levisky

1988

Clinical Laboratory Analysis

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The Tandem ICON Strep A@ (TISA; Hybritech, Inc.) was compared to culture for detection of group A streptococci, using throat swabs obtained from patients with symptoms of pharyngitis. Each swab was first inoculated to a 5% sheep blood agar plate and then tested for streptococcal antigen using the TISA. However, TlSA specificity ranged from 93.6% in one of 3 lots tested to 99.1% in antoher (P < 0.005). The PVP of the former lot was 76.3%, whereas that of the latter was 95.6% (P < 0.005). The relative lack of sensitivity and lot-to-lot variation observed with the TlSA in this study indicate that the product is not a suitable alternative to a competently performed throat culture.

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Five vs Ten Days of Penicillin V Therapy for Streptococcal Pharyngitis

Gerber¹,

Randolph²,

Chanatry³

et al. 1987

Arch Pediatr Adolesc Med

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Evaluation of aerobic and anaerobic methods for recovery of streptococci from throat cultures

Cited by 19 publications

References 6 publications

Suitability of a Throat Culture Method for Evaluation of Group A Streptococcal Antigen Detection Kits

Suitability of a Throat Culture Method for Evaluation of Group A Streptococcal Antigen Detection Kits

Performance of the tandem ICON strep A® enzyme immunoassay system for detection of group a streptococci from oropharyngeal swabs

Five vs Ten Days of Penicillin V Therapy for Streptococcal Pharyngitis

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