Evaluation of adjuvants for a candidate conjugate vaccine against benzo[a]pyrene

Schellenberger, Mario T.; Farinelle, Sophie; Willième, Stéphanie

doi:10.4161/hv.7.0.14579

Cited by 15 publications

(9 citation statements)

References 39 publications

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“…On the basis of all these data authors offered the strategy of vaccination against Cg to induce the mucosal Abs for the cancer immunoprevention (Silbart et al, 1997, Schellenberger et al, 2011, Černohorská et al, 2012). Unfortunately the effects of immunization with PE on the S functions were not studied, while Abs against PE used widely for their detection (Qu et al, 2016).…”

Section: Antibodies Against Chemical Carcinogens and Steroidsmentioning

confidence: 99%

Immunomodulation of carcinogens-induced steroids-dependent human diseases

Глушков

Поленок

2019

Saudi Journal of Biological Sciences

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The experimental and clinical data about antibodies against environmental chemical carcinogens and endogenous steroids are represented. The conception of immunomodulation of carcinogens- and steroids-dependent human diseases is proposed. It is postulated that antibodies to polycyclic aromatic hydrocarbons and heterocyclic amines in cooperation with antibodies to cholesterol, sex hormones, mineralo- and glucocorticoids stimulate or inhibit cancer, malformation, cardiovascular and autoimmune diseases depending on their personal combination. It is recommended to use immunoassay of these antibodies for the human diseases prediction. The alternative approaches for prevention using the probiotics transformed by anti-carcinogen antibodies are substantiated.

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Section: Antibodies Against Chemical Carcinogens and Steroidsmentioning

confidence: 99%

Immunomodulation of carcinogens-induced steroids-dependent human diseases

Глушков

Поленок

2019

Saudi Journal of Biological Sciences

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“…Основной причиной возникновения рака легкого (РЛ) является воздействие на эпителий бронхов химических канцерогенов, таких как полициклические ароматические углеводороды, в частности бензо[а]пирена (Вр) [37]. Применение антиканцерогенных вакцин рассматривается как перспективное направление профилактики рака [11,38,39].…”

Section: Introductionunclassified

Antibodies to Benzo[a]pyrene, Estradiol and Progesterone and Gene Polymorphisms of Cytokines: Associations With Lung Cancer in Men

Глушков¹,

Поленок²,

Гордеева³

et al. 2018

Med. immunol.

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Previous studies have revealed associations of antibodies, specific to chemical carcinogens and steroid hormones with lung cancer in men. However, the mechanisms of their formation and action were remained unclear. In particular, the relationships between antibodies and gene polymorphisms of cytokines were unknown. The purpose of this study was to identify possible associations between occurrence of A class antibodies, specific to benzo[a]pyrene, estradiol and progesterone (IgA-Bp, IgA-Es and IgA-Pg), and frequency of genetic polymorphisms of IL1RN VNTR, IL1В (rs1143634, rs16944), IL4 VNTR, IL6 (rs1800795), IL10 (rs1800896), TNFA (rs1800629, rs361525) genes in healthy male smokers and lung cancer patients. We have examined 381 men with non-small cell lung cancer and 158 apparently healthy donors without respiratory diseases. A non-competitive solid phase immunoassay of antibodies was performed. Analysis of polymorphic loci of IL1RN (VNTR, intron 2), IL4 (VNTR, intron 3) was performed by means of conventional PCR; IL1В (rs1143634, rs16944), IL6 (rs1800795) SNPs were detected by RFLP, and IL10 (rs1800896), TNFA (rs1800629, rs361525) genotyping was carried out with TaqMan Real-time PCR. Results of the study have shown that the proportion of cases with high level of IgA-Pg and low levels of both IgA-Bp and IgA-Es among the lung cancer patients was lower than in healthy men (OR = 0.31, p < 0.0001). Vice versa, the ratio of cases with high levels of both IgA-Bp and IgA-Es and low levels of IgA-Pg was higher in lung cancer patients (OR = 3.6, p < 0.0001). No relationships were revealed between the levels of antibodies, and rates of genetic polymorphisms for the studied cytokines in both groups of men. At the same time, the detected associations of IgA-Bp, IgA-Es and IgA-Pg with lung cancer proved to be significant only in carriers of certain cytokine genotypes, e.g., in AG IL10 heterozygotes (OR = 5.1, p < 0.0001). In conclusion, these results provide indirect evidence that IgA-Bp and IgA-Es could stimulate initiation and promotion of lung carcinogenesis. On the contrary, IgA-Pg could inhibit the promotion of carcinogenesis. Immunoassay of these antibodies combined with molecular biology studies of IL10 gene variants are recommended for the lung cancer risk assessment.

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“…Still, there are only few data available on efficacy of the Montanide ™ in comparison with FA aiming to replace FA in research. And these studies compared the adjuvants under slightly different aspects, for example with the aim to enhance adjuvanticity of peptidoglycan monomer (PGM) by protecting it from the fast degradation and metabolic clearance or inducing carcinogen‐neutralizing antibodies . In this study, we compare the humoral immune response of mice vaccinated with our anti‐ S. aureus candidate protein hp2160 in combination with either Montanide ™ ISA71, ISA 206 or Freund's adjuvant in terms of IgG subclass analysis, animal condition and furthermore the survival rate after S. aureus infection.…”

Section: Introductionmentioning

confidence: 99%

Montanide ISA 71 VG is Advantageous to Freund's Adjuvant in Immunization Against S. aureus Infection of Mice

et al. 2015

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The enormous capacity of Staphylococcus aureus to acquire antibiotic resistance makes it a permanent task to search for and to develop new anti-infectives. One of the possible approaches is the early active immunization of risk patients and animal stocks to prevent S. aureus infections. Based on a S. aureus proteome screen with S. aureus-specific human antiserum, we have previously identified several anchorless cell wall proteins to be used as novel vaccine candidates. To develop an efficient anti-S. aureus vaccine, the supplemented adjuvants Montanide TM ISA 71 VG and ISA 206 were compared to Freund's adjuvant in terms of handling, induction of cytokine profile, triggering antigen-specific immunoglobulin production of different IgG subclasses and provision of increased survival rates in our S. aureus sepsis mouse model. Immunization with ISA 71 VG in comparison with Freund's adjuvant induced slightly delayed but comparably strong increase of antigen-specific antibody titres and conferred protective effect against S. aureus challenge. In contrast using ISA 206 as adjuvant, significantly lower IgG titres and consequently, no protective effect against S. aureus infection were observed. Handling and tolerability of the Montanide is superior to Freund's adjuvant. Montanide TM ISA 71 VG can serve as an effective adjuvant replacement for Freund's adjuvant in research with a prospective usage in animal and human vaccines against bacterial pathogens.

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Evaluation of adjuvants for a candidate conjugate vaccine against benzo[a]pyrene

Cited by 15 publications

References 39 publications

Immunomodulation of carcinogens-induced steroids-dependent human diseases

Immunomodulation of carcinogens-induced steroids-dependent human diseases

Antibodies to Benzo[a]pyrene, Estradiol and Progesterone and Gene Polymorphisms of Cytokines: Associations With Lung Cancer in Men

Montanide ISA 71 VG is Advantageous to Freund's Adjuvant in Immunization Against S. aureus Infection of Mice

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