Evaluation of adenosine deaminase activity in serum and pleural fluid of pulmonary tuberculosis patients with pleural effusion

Malempati, Uma D.; Medooru, Kusuma K.

doi:10.18203/2320-6012.ijrms20184046

Cited by 2 publications

(2 citation statements)

References 14 publications

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“…Malempati UD et al evaluated the effective treatment response to ATT by estimating the serum ADA activity, one month after the treatment initiation. 11 Before the initiation of ATT, the serum and pleural fluid ADA activities were elevated, with pleural fluid ADA activity elevated more than that of the serum in pulmonary tuberculosis patients with pleural effusion. The serum ADA activity was decreased in pulmonary tuberculosis patients treated for one month with antitubercular treatment.…”

Section: Resultsmentioning

confidence: 90%

Role of adenosine deaminase in monitoring tubercular pleural effusion

Hazarika

Sharma

Kumar

et al. 2019

Int J Community Med Public Health

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Background: Tuberculosis is a common cause of pleural effusion especially in countries like India. ADA (adenosine deaminase) is predominantly an enzyme, that catalyses the conversion of adenosine to ionosine. Usually patients with tuberculous pleural effusion have ADA level >40 U/L.Methods: This is a prospective, observational study conducted in Department of Pulmonary Medicine, Gauhati Medical College and Hospital, Guwahati from September 2016 to September 2017. 45 patients with pleural fluid ADA levels >40 U/L were selected with diagnosis of tubercular pleural effusion. Pleural fluid was analysed for cytological, biochemical and microbiological parameters along with ADA and malignant cell cytology. Anti-tubercular treatment (ATT) was started and pleural fluid ADA level were repeated after 15 days of ATT.Results: Pleural fluid ADA levels before the start of ATT intake and after 15 days of ATT intake were statistically analysed. Among 45 patients, 38 were male and 7 females. Mean age of the patients was 45.42±16.43 years. Mean pleural fluid ADA level before starting ATT was 64.49±31.78 U/L. After 15 days of ATT intake mean pleural fluid ADA level was 36.11±10.42 U/L, p value was statistically significant (p<0.05).Conclusions: Pleural fluid ADA significantly decreased after 15days of initiation of anti-tubercular treatment. Pleural fluid ADA can be a useful tool as a follow up biomarker in cases of tubercular pleural effusion.

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Section: Resultsmentioning

confidence: 90%

Role of adenosine deaminase in monitoring tubercular pleural effusion

Hazarika

Sharma

Kumar

et al. 2019

Int J Community Med Public Health

View full text Add to dashboard Cite

show abstract

“…Mandal and Chavan (2016) found in their study that, ESR was elevated in 87% and normal in 26% of pulmonary TB patients. 20 13,15,16,17 The majority of the participants were male in this study. This might be because of more exposure of economically productive males to the external environment.…”

Section: Resultsmentioning

confidence: 95%

Diagnostic Role of Serum Adenosine Deaminase In Smear-negative Pulmonary Tuberculosis

Ali,

Azad

2024

Bangla J Med

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Background: Diagnosis of tuberculosis is not always easy, particularly if it is a case of sputum smear-negative pulmonary tuberculosis (SNPTB). Patients with respiratory symptoms resembling SNPTB are difficult to differentiate based on clinical features, chest X-ray, and Xpert MTB/RIF negativity. So, additional diagnostic test with high sensitivity and specificity is needed to increase the yield of the ongoing diagnostic strategy for SNPTB. Adenosine deaminase (ADA) is now being widely used for the diagnosis of TB particularly in effusion fluids due to its simplicity, low cost, and quick available results, it is not always possible to access effusion fluids and therefore, it would be helpful to take advantage of serum levels. Therefore, the purpose of the study was to assess the role of serum ADA in the diagnosis of SNPTB. Methods: This cross-sectional analytical study was conducted in Dhaka Medical College & Hospital, Dhaka from March 2019 to September 2021. A total of 140 patients were included in this study and divided into two groups according to selection criteria: Group I (SNPTB, n=62), and Group II (non-TB pulmonary diseases, n=78). ADA estimation was carried out using the sensitive colorimetric method described by Guisti and Galanti with a BIOSIC kit. After the collection of all the required data, analysis was done by SPSS 24.0. Results: The mean age of the study patients was 48.02 ± 9.60 years (23-73 years) with male predominance in both Group I and Group II (71 % and 60.3%, respectively, p>0.05). Non-TB pulmonary cases were significantly older than SNPTBpatients (52 ± 8.56 vs 43.02 ± 8.49 years, p<0.001). SNPTB patients had a significantly higher frequency of cough, fever, and weight loss compared to non-TB pulmonary cases (p<0.05). In contrast, chest pain and shortness of breath were more frequent in Group II than in Group I (p<0.05). Serum ADA was significantly higher among SNPTB patients compared to non-TB pulmonary cases (48.16 ± 12.13 vs 18.64 ± 7.85 IU/L, p<0.001). ROC analysis of serum ADA in the diagnosis of patients with SNPTB found an AUC of 0.9850 (95% CI, 0.969-1.00) which was statistically significant (p<0.001). A cut-off value of serum ADAe”33 IU/L showed sensitivity, specificity, NPV, PPV, and accuracy of 93.55%, 94.87%, 94.87%, 93.55%, and 94.29%, respectively to correctly diagnose SNPTB cases. Conclusion. This study finding stated that serum ADA may be a useful marker to distinguish SNPTB from non-TB respiratory diseases. However, further study with a more generalized study population is recommended. Bangladesh J Medicine 2024; 35: 61-69

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Evaluation of adenosine deaminase activity in serum and pleural fluid of pulmonary tuberculosis patients with pleural effusion

Cited by 2 publications

References 14 publications

Role of adenosine deaminase in monitoring tubercular pleural effusion

Role of adenosine deaminase in monitoring tubercular pleural effusion

Diagnostic Role of Serum Adenosine Deaminase In Smear-negative Pulmonary Tuberculosis

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