Evaluation of Acute Phase Proteins in Clinically Healthy Dairy Cows in Perinatal Period and During Lactation

Abstract

The estimation of acute phase proteins (APP), which are recognized as inflammation markers is a good method for animal health monitoring. Several factors such as obesity, age and sex are also known to modulate APP status. We evaluated the influence of pregnancy and lactation in 65 clinically healthy dairy Holstein-Friesian dairy cows, 2nd÷4th lactation, chosen from 3 different dairy farms located in South West part of Poland. Bovine C-reactive protein (CRP), haptoglobin and fibrinogen were assayed using commercial ELISA kits. The highest values of CRP and haptoglobin were observed in cows during the first month after calving. The highest concentrations of fibrinogen was found in a group of cows prior to expected date of parturition and the level of this protein in blood plasma was decreasing during lactation. The significant differences of analyzed APPs among cows before delivery, during first month after calving and in lactation (1-3 months after delivery) suggested that factors like pregnancy and stage of lactation would have an influence on their concentration.

Evaluation of serum C-reactive protein concentration as a marker of impending parturition and correlation with progesterone profile in peri-partum bitches

“…In women, the CRP concentrations are generally higher during pregnancy, and a significant increase can be observed in the postpartum period, approximately10folds as high as the concentrations during the second and third trimester values (Skarżyńska et al, 2018). CRP concentration was also significantly higher during the first month after calving than in the last trimester of pregnancy in cows (Dębski et al, 2016).…”

Immunometabolic response in Egyptian water buffalo cows during the transition period

“…APPs play a major role in the innate immune response and are primarily coordinated by cytokine-mediated production, which serve to both prevent inflammation and initiate inflammatory processes, as well as eliminate potential pathogens and contribute to healing [ 42 ]. The most important APPs in ruminant animals are Hp and CRP that provide an early nonspecific defense mechanism against injury, infection, or inflammation before the response of specific immunity [ 43 ]. Hp is recognized as a marker of inflammation in dairy cows; however, its concentration is also found to increase in a week after calving [ 44 ].…”

Concentration of C-reactive protein and haptoglobin in cows with subclinical endometritis

“…Отримані нами дані співпадають з результатами попередніх досліджень [30, 31], у яких максимальну концентрацію С-реактивного білка теж спостерігали у корів із запальним процесом (гострий, хронічний мастит, метрит, ендометрит та ін.). За результатами досліджень [32], Рис. 1.…”

“…Зростання концентрації гаптоглобіну у корів із субклінічним ендометритом пов'язують із посиленням синтезу цитокінів у відповідь на дію мікроорганізмів [10]. У кількох дослідженнях [32,34], спостерігали зростання гаптоглобіну у корів протягом післяродового періоду, що пояснюють реакцією на обсіменіння мікроорганізмами в процесі інволюції. Слід зазначити, що в нашому дослідженні найвищі концентрації гаптоглобіну та С-реактивного білка спостерігали одночасно за субклінічного ендометриту.…”

“…Слід зазначити, що в нашому дослідженні найвищі концентрації гаптоглобіну та С-реактивного білка спостерігали одночасно за субклінічного ендометриту. У раніше опублікованих дослідженнях [32], вказують на кореляцію (R2=0.32) цих білків гострої фази.…”