Evaluation of acute oral toxicity, anti-diabetic and antioxidant effects of Aloe vera flowers extract

Elkomy, Nesreen M.I.M.; El-Shaibany, Amina; Elnagar, Gehad M.; Abdelkhalek, Ahmed S.; Al-Mahbashi, Hassan M.; Elaasser, Mahmoud M.; Raweh, Salwa Mohammed; Aldiyarbi, Maha A.; Raslan, Ali E.

doi:10.1016/j.jep.2023.116310

Cited by 8 publications

(4 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…GA, characterized by its phenolic and carboxylic acid properties, can potentially mitigate inflammation-related diseases by modulating redox status and regulating the intestinal microbiota [ 26 , 31 ]. In vivo studies have affirmed the anti-inflammatory effects of GA in conditions such as obesity, diabetes, and colitis [ 27 , 29 , 32 ]. Studies have shown that its main anti-inflammatory mechanisms include suppressing the activation of factors involved in the transcription and transduction of signals, reducing the expressions of inflammatory mediators and inhibiting the phosphorylation or transfer of p65-NF-κB [ 33 ‒ 35 ].…”

Section: Discussionmentioning

confidence: 99%

“…GA has anti-inflammatory effects by reducing proinflammatory mediators, inhibiting the expressions of nuclear transcription factors and downregulating downstream inflammatory targets [ 26 , 27 ]. In addition to its anti-inflammatory effects, GA has been shown to have pharmacological effects on tumors, diabetes, and obesity [ 27 ‒ 29 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Gallic acid attenuates LPS-induced inflammation in Caco-2 cells by suppressing the activation of the NF-κB/MAPK signaling pathway

Chu,

Ru,

Chen

et al. 2024

ABBS

View full text Add to dashboard Cite

Inflammatory bowel disease (IBD) is a chronic inflammatory disease characterized by intestinal barrier dysfunction, inflammatory synergistic effects and excessive tissue injury. Gallic acid (GA) is renowned for its remarkable biological activity, encompassing anti-inflammatory and antioxidant properties. However, the underlying mechanisms by which GA protects against intestinal inflammation have not been fully elucidated. The aim of this study is to investigate the effect of GA on the inflammation of a lipopolysaccharide (LPS)-stimulated human colon carcinoma cell line (Caco-2) and on the intestinal barrier dysfunction, and explore the underlying molecular mechanism involved. Our findings demonstrate that 5 μg/mL GA restores the downregulation of the mRNA and protein levels of Claudin-1, Occludin, and ZO-1 and decreases the expressions of inflammatory factors such as IL-6, IL-1β and TNF-α induced by LPS. In addition, GA exhibits a protective effect by reducing the LPS-enhanced early and late apoptotic ratios, downregulating the mRNA levels of pro-apoptotic factors ( Bax , Bad , Caspase-3 , Caspase-8 , and Caspase-9 ), and upregulating the mRNA levels of anti-apoptotic factor Bcl-2 in Caco-2 cells. GA also reduces the levels of reactive oxygen species increased by LPS and restores the activity of antioxidant enzymes, namely, superoxide dismutase and catalase, as well as the level of glutathione. More importantly, GA exerts its anti-inflammatory effects by inhibiting the LPS-induced phosphorylation of key signaling molecules in the NF-κB/MAPK pathway, including p65, IκB-α, p38, JNK, and ERK, in Caco-2 cells. Overall, our findings show that GA increases the expressions of tight junction proteins, reduces cell apoptosis, relieves oxidative stress and suppresses the activation of the NF-κB/MAPK pathway to reduce LPS-induced intestinal inflammation in Caco-2 cells, indicating that GA has potential as a therapeutic agent for intestinal inflammation.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Gallic acid attenuates LPS-induced inflammation in Caco-2 cells by suppressing the activation of the NF-κB/MAPK signaling pathway

Chu,

Ru,

Chen

et al. 2024

ABBS

View full text Add to dashboard Cite

show abstract

“…The antioxidant activity of CLLEO was assessed by three methods with different mechanisms; the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay according to Elkomy et al [67], 2,2 -azinobis(3-ethylbenzo-thiazoline-6-sulfonic acid) radical cation (ABTS•+) scavenging assay according to Ling et al [68] and ferric reducing antioxidant power (FRAP) assay according to Elaasser et al [69]. The experiments were performed in triplicate and average values were considered.…”

Section: Antioxidant Activity Assaysmentioning

confidence: 99%

Chamaecyparis lawsoniana Leaf Essential Oil as a Potential Anticancer Agent: Experimental and Computational Studies

Fikry

Orfali

Elbaramawi

et al. 2023

Plants

View full text Add to dashboard Cite

Cancer remains one of the leading causes of death worldwide, affected by several factors including oxidative stress; and although conventional synthetic medicines have been used to treat cancer, they often result in various side effects. Consequently, there is a growing need for newer, safer and more effective alternatives, such as natural plant products. Essential oils (EOs) are one such alternative, offering a wide range of bioactivities, including antibacterial, antiviral, antioxidant, and anticancer properties. Accordingly, the objective of the present study was to investigate the chemical composition, as well as the antioxidant and anticancer properties of the leaf essential oil of Chamaecyparis lawsoniana (CLLEO) belonging to the Cupressaceae family. Totally, 59 constituents were identified by gas chromatography-mass spectrometry (GC-MS) analysis. cis-Abienol, trans-ferruginol, α-cadinol, δ-muurolene and α-pinene were the major components. The in vitro cytotoxicity study against human breast (MCF-7), colon (HCT-116), lung (A-549), hepatocellular (HepG-2) carcinoma cells using MTT assay indicated a promising cytotoxic activity against all the tested cancer cells, particularly HepG-2, with significant selectivity indices. CLLEO exhibited weak antioxidant activity according to the DPPH, ABTS and FRAP assays. In silico docking of these constituents against the epidermal growth factor receptor (EGFR), the myeloid cell leukemia-1 (Mcl-1) and caspase-8 using Molecular Operating Environment (MOE) software demonstrated good binding affinities of the components with the active site of these targets. These findings suggested using CLLEO, or its individual components, as a potentially viable therapeutic option for managing cancerous conditions.

show abstract

“…In addition, Aloe Vera has antioxidant properties that neutralize free radicals caused by exposure to electromagnetic radiation, which further enhances its protective effects (Nesreen et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

Efficacy of Sabila (Aloe Vera) in Electromagnetic Absorption in Households

Rene Antonio Hinojosa Benavides,

Demetrio Soto Carbajal,

Andrés Zósimo Ñahui Gaspar

et al. 2023

J. Adv. Zool.

View full text Add to dashboard Cite

A documentary review was carried out on the production and publication of research papers related to the study of the variables Aloe Vera and Radiation Absorption. The purpose of the bibliometric analysis proposed in this document was to know the main characteristics of the volume of publications registered in the Scopus database during the period 2012-2022, achieving the identification of 18 publications. The information provided by this platform was organized through graphs and figures categorizing the information by Year of Publication, Country of Origin, Area of Knowledge and Type of Publication. Once these characteristics have been described, the position of different authors towards the proposed theme is referenced through a qualitative analysis. Among the main findings made through this research, it is found that India, with 11 publications, was the country with the highest scientific production registered in the name of authors affiliated with institutions of that nation. The Area of Knowledge that made the greatest contribution to the construction of bibliographic material referring to the study of the variables Aloe Vera and the absorption of radiation, was Pharmacology, Toxicology and Pharmaceutical with 6 published documents, the same presented under the theories of Chemistry. The most used Type of Publication during the period indicated above were Journal Articles with 61% of the total scientific production.

show abstract

Evaluation of acute oral toxicity, anti-diabetic and antioxidant effects of Aloe vera flowers extract

Cited by 8 publications

References 42 publications

Gallic acid attenuates LPS-induced inflammation in Caco-2 cells by suppressing the activation of the NF-κB/MAPK signaling pathway

Gallic acid attenuates LPS-induced inflammation in Caco-2 cells by suppressing the activation of the NF-κB/MAPK signaling pathway

Chamaecyparis lawsoniana Leaf Essential Oil as a Potential Anticancer Agent: Experimental and Computational Studies

Efficacy of Sabila (Aloe Vera) in Electromagnetic Absorption in Households

Contact Info

Product

Resources

About

Evaluation of acute oral toxicity, anti-diabetic and antioxidant effects of Aloe vera flowers extract

Cited by 8 publications

References 42 publications

Gallic acid attenuates LPS-induced inflammation in Caco-2 cells by suppressing the activation of the NF-&kappa;B/MAPK signaling pathway

Gallic acid attenuates LPS-induced inflammation in Caco-2 cells by suppressing the activation of the NF-&kappa;B/MAPK signaling pathway

Chamaecyparis lawsoniana Leaf Essential Oil as a Potential Anticancer Agent: Experimental and Computational Studies

Efficacy of Sabila (Aloe Vera) in Electromagnetic Absorption in Households

Contact Info

Product

Resources

About

Gallic acid attenuates LPS-induced inflammation in Caco-2 cells by suppressing the activation of the NF-κB/MAPK signaling pathway

Gallic acid attenuates LPS-induced inflammation in Caco-2 cells by suppressing the activation of the NF-κB/MAPK signaling pathway