Evaluation of Acoustical Parameter Sensitivity to Age-Related and Osteoarthritic Changes in Articular Cartilage Using 50-MHz Ultrasound

Chérin, Emmanuel; Saied, A.; Laugier, Pascal; Netter, Patrick; Berger, G.

doi:10.1016/s0301-5629(97)00289-5

Cited by 122 publications

(94 citation statements)

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“…Acquisition system. The US signals backscattered by the cartilage patellae were acquired using a 55-MHz scanning acoustic microscope which has been described in previous reports (9,10). The system operated with a polymer focused transducer (Toray Techno Co., Shiga-ken, Japan) providing axial and lateral resolutions of 20 m and 65 m, respectively, at focus (7.5 mm).…”

Section: Methodsmentioning

confidence: 99%

“…The RF signals were processed in the frequency domain in order to estimate the integrated reflection coefficient (IRC) from the cartilage surface as well as the apparent integrated backscatter from the cartilage internal structure (cartilage matrix) (AIB cartilage ) and from the cartilage-bone interface (AIB bone ). These parameters, which are device-and operator-independent, were measured using a standard substitution method that compensates for the apparatus transfer function (9).…”

Section: Methodsmentioning

confidence: 99%

“…Since it was estimated over a region of interest (ROI) located in the central part of the patella, where cartilage surface was perpendicular to the incident sound beam direction, this parameter was mainly related to the composition and microarchitecture of the superficial layer of cartilage and to surface roughness (9). From each RF line of the ROI (80 m in the transverse direction ϫ 400 m in the sagittal direction), the signal reflected by cartilage surface was extracted using a 40 m-wide window.…”

Section: Methodsmentioning

confidence: 99%

“…From each RF line backscattered by the central region of the patella, the signal from the cartilage internal structure was selected using a window located 20 m beneath the cartilage surface. The apparent backscatter was calculated in dB over a volume of tissue approximately 240 m wide ϫ 1 mm long ϫ 120 m thick and was then integrated in the 23-66 MHz frequency bandwidth to estimate the AIB cartilage value (9).…”

Section: Methodsmentioning

confidence: 99%

“…Recent research suggests that high-frequency US may also serve ex vivo as a useful means for the investigation of cartilage matrix structural changes occurring under var-ious experimental and clinical conditions (e.g., in the growth process and in osteoarthritis) (9)(10)(11)(12). In particular, our group has shown that 50 MHz US backscatter from cartilage matrix is not sensitive to changes in PG content, but is related to collagen network organization (13).…”

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confidence: 87%

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Effects of antiinflammatory drugs on arthritic cartilage: A high‐frequency quantitative ultrasound study in rats

Jaffre¹,

Watrin²,

Lœuille³

et al. 2003

Arthritis & Rheumatism

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Objective. To evaluate the ability of 55-MHz quantitative ultrasound (US) to detect the in vivo effects of experimental arthritis, as well as those of two antiinflammatory drugs, naproxen (NPX) and dexamethasone (DEX), on cartilage and subchondral bone.Methods. Arthritis was induced in both knees of 108 rats by intraarticular injection of zymosan (ZYM). Two groups of arthritic rats (n ‫؍‬ 36 per group) were treated daily with either NPX (10 mg/kg/day) or DEX (0.1 mg/kg/day). Using a 3-dimensional US microscope, patellae were explored in vitro on days 5, 14, and 21 after injections. US assessment included the analysis of quantitative indices of local modifications involving cartilage and bone: integrated reflection coefficient (IRC) from the cartilage surface and apparent integrated backscatter from the cartilage internal structure (cartilage matrix) (AIB cartilage ) and the cartilage-bone interface (AIB bone ).Results. ZYM induced articular surface fibrillation that resulted in a decrease in IRC at all times (P < 0.02) and in an increase in AIB bone on days 5 and 14 (P < 0.005). Fibrillation was not changed by NPX administration, while it disappeared following DEX treatment. Cartilage-bone interface alterations were prevented by DEX and partially compensated for by NPX. Cartilage matrix echogenicity decreased with time in all groups due to maturation (P < 0.05), except in DEX-treated rats.Conclusion. Quantitative 55 MHz US allowed detection of early cartilage and bone lesions due to experimental arthritis, and also allowed detection of the effects of antiinflammatory drugs. NPX seemed to have an effect on subchondral bone lesions, but not on cartilage. DEX appeared to repair articular surface and bone, but prevented animal growth and cartilage maturation.In rheumatoid arthritis (RA), synovial inflammation and pannus releasing matrix metalloproteinases, oxygen-derived free radicals, cytokines, and prostaglandins degrade articular cartilage. These mediators provoke proteoglycan (PG) synthesis inhibition, cartilage destruction, bone erosions, and ancillary progressive articular loss of function (1,2). They are also responsible for the swelling and pain in arthritic joints. Nonsteroidal antiinflammatory drugs (NSAIDs) and oral steroids reduce the RA symptoms of joint pain and swelling. However, evidence in clinics and from in vitro studies that NSAIDs and steroids can prevent the progression of chronic, destructive arthritis is both sparse and controversial (3-7). Furthermore, most in vitro studies rely on destructive techniques (e.g., histology and biochemistry), and the criteria for determining cartilage degradation remain qualitative.There is increasing interest in the use of ultrasonography in rheumatology, since ultrasound (US) is noninvasive, safe, more accurate than clinical evaluation of synovitis and cartilage thickness in small joints, and can be used repeatedly in RA patients (8). Recent research suggests that high-frequency US may also serve ex vivo as a useful means for the investigation of cartilage ma...

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 87%

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