Evaluation of Accuracy of Multitest Micromethod System for Identification of Enterobacteriaceae

Washington, John A.; Yu, Pauline K. W.; Martin, William J.

doi:10.1128/aem.22.3.267-269.1971

Cited by 42 publications

(29 citation statements)

References 5 publications

(7 reference statements)

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“…) tests of 96% with Enterobacteriaceae (Washington et al 1971) and 90% with Aeromonas genospecies (Carnahan et al 1991b). A characteristic of the miniaturized format was the speed at which tests became positive.…”

Section: Discussionmentioning

confidence: 99%

Miniaturized tests for computer-assisted identification of motile Aeromonas species with an improved probability matrix

Carson¹,

Wagner²,

Wilson³

et al. 2001

J Appl Microbiol

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Aims: To develop miniaturized tests for the phenotypic identi®cation of motile Aeromonas species using an improved probability matrix. Methods and Results: Conventional tests were miniaturized for use in 96-well plates, and their performance assessed using 60 aeromonads comprising type and reference strains as well as clinical, ®sh and water isolates. A revised probability matrix for Aeromonas hybridization groups 1±14, including A. allosaccharophila, A. bestiarum, A. encheleia and A. popof®i, was developed. Using 26 tests, all the reference strains were correctly identi®ed with the revised probability matrix, and 80% of the isolates were correctly identi®ed at a Willcox probability level of 95%. Conclusions: The compact test format, coupled with a robust identi®cation matrix, provides a convenient basis for identifying motile aeromonads. Signi®cance and Impact of the Study: The identi®cation system for identifying aeromonads will be of use to medical and veterinary laboratories undertaking disease diagnosis.

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Section: Discussionmentioning

confidence: 99%

Miniaturized tests for computer-assisted identification of motile Aeromonas species with an improved probability matrix

Carson¹,

Wagner²,

Wilson³

et al. 2001

J Appl Microbiol

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“…1,1996), which now includes the variations in arabinose fermentation so often seen with isolates of Y. pestis, should increase the accuracy of this system. In previous studies, API correctly identified 88 to 99.4% of the enteric, nonfermentative bacteria tested (3,(5)(6)(7)(8)(9), and API 20NE correctly identified 75.3% of these bacteria (10). These older systems clearly misidentify the less reactive bacteria, which could lead to misdiagnosis.…”

mentioning

confidence: 94%

“…No rapid biochemical identification system should stand alone as a diagnostic tool. Whether or not bacterial isolates have clinical significance, their correct identification can help rule out potential pathogens in a patient sample (9). Conventional bacteriology is still needed in the laboratory diagnosis of plague.…”

mentioning

confidence: 99%

Identification of Yersinia pestis by BBL Crystal Enteric/Nonfermenter Identification System

1996

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“…hydrophila, suggests that the MPN method may not be reliable since recovery of the non-Enterobacteriaceae strains is not considered to be evidence of unsanitary conditions. Furthermore, it is possible that acquisition of lac+ plasmids by these strains may account for the phenomenon (Sutter & Foecking 1962;Falkow et al 1964;Washington et a/. 1971).…”

Section: Austin Et a Lmentioning

confidence: 99%

Numerical Taxonomy Analysis of Bacteria Isolated from the Completed ‘Most Probable Numbers’ Test for Coliform Bacilli

Austin¹,

Hussong²,

Weiner³

et al. 1981

Journal of Applied Bacteriology

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Lactose-fermenting bacteria were isolated from oyster, water and sediment samples tested to determine the Most Probable Number (MPN) of coliforms present. The completed test series was used to enumerate the total number of coliforms. The strains from the completed tests of the MPN analysis were compared with named reference cultures, for which 100 phenotypic characters were recorded and the taxonomic data analysed by methods of numerical taxonomy. The isolates were recovered in 13 phenetic groups, defined at or above the 80% similarity level. Nine phena were identified as Acinetohacter calcoaceticus, Chromobacterium violaceum, Citrobacter freundii, Erwinia herbicola, Escherichia coli, Hafnia alvei, Klebsiella pneumoniae, Serratia liquefaciens, and Ser. marcescens. Of these, Ac. calcoaceticus and C. violaceum were isolated exclusively from oyster tissue samples and strains of the other taxa from both water and oyster samples. Some strains of Esch. coli, Er. herbicola, H. alvei and bacteria that were not Enterobacteriaceae were found to lose the ability to ferment lactose, following storage on laboratory media. Identifications from diagnostic keys, rapid identification systems and IMViC patterns were not always in agreement.

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Evaluation of Accuracy of Multitest Micromethod System for Identification of Enterobacteriaceae

Cited by 42 publications

References 5 publications

Miniaturized tests for computer-assisted identification of motile Aeromonas species with an improved probability matrix

Miniaturized tests for computer-assisted identification of motile Aeromonas species with an improved probability matrix

Identification of Yersinia pestis by BBL Crystal Enteric/Nonfermenter Identification System

Numerical Taxonomy Analysis of Bacteria Isolated from the Completed ‘Most Probable Numbers’ Test for Coliform Bacilli

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