Evaluation of Abbott CMV-M enzyme immunoassay for detection of cytomegalovirus immunoglobulin M antibody

Abstract

The Abbott CMV-M enzyme immunoassay (EIA) for the qualitative determination of immunoglobulin M (IgM) antibody to cytomegalovirus in human serum was compared with the indirect fluorescent-antibody (IFA) test on 338 human serum specimens. Discordant specimens were evaluated by IFA following isolation of IgM fractions. Discordant specimens remaining after IFA testing were evaluated by an IgM-specific EIA (CYTOMEGELISA M; M.A. Bioproducts). After resolution of discordant specimens, the CMV-M EIA was 94.7% sensitive and 99.1% specific.

Maternal cytomegalovirus immune status and hearing loss outcomes in congenital cytomegalovirus-infected offspring

“…CMV IgG antibody was determined by enzyme-linked immunosorbent assay and anticomplement immunofluorescence [34]. CMV IgM antibody was determined by enzyme linked PLOS ONE…”

“…CMV IgG antibody was determined by enzyme-linked immunosorbent assay and anticomplement immunofluorescence [ 34 ]. CMV IgM antibody was determined by enzyme linked immunosorbent assay and radioimmunoassay, as previously described [ 33 ].…”

“…In a sub-study of CMV infection in mothers and their infants, during 1981 to 1986, 4,578 pregnant women who were also patients of a private obstetric group practice participating in The Houston Congenital CMV Longitudinal Study were enrolled and screened prospectively for CMV IgM and IgG antibodies, beginning at the first obstetrical visit, after providing informed consent. The demographics and seroprevalence results of the serologic screening have been previously published [34]. In addition, from 1986 to 1992, mothers with identified CMV infections during pregnancy who were referred to us from other obstetrical practices were also included in this study [31].…”

“…Maternal sera for determination of CMV IgG and IgM antibodies were obtained as early in pregnancy as possible, and repeated at each trimester and at delivery when possible [34]. Definitions for the type of maternal CMV infection were adapted from prospective serologic and demographic studies of CMV infection in pregnant women that were previously published and contemporary to the time of the serologic screening process for this study [31].…”

Cytomegalovirus testing: Antibody determinations and virus cultures with recommendations for use

“…Conversely, IgM may be present in patients, especially those with AIDS, who have reactivated CMV but do not have disease (21). The potential problem of rheumatoid factors that nonspecifically bind to the cell monolayer and cause a false-positive reaction should be kept in mind when interpreting this assay (28). Some adults and neonates with congenital infections with other viruses have IgM rheumatoid factors present in their serum (22).…”