Evaluation of a Visual Enzyme-Linked Immunosorbent Assay (Elisa) for Pregnancy Detection in Four Ungulate Species

Dvornicky-Raymond, Zachary T.; Donnelly, Kyle A.; Emerson, Jessica A.; Penfold, Linda M.; Citino, Scott B.

doi:10.1638/2020-0106

Cited by 4 publications

(3 citation statements)

References 31 publications

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“…Inflammatory factors: we collected 5 mL of fasting cubital venous blood from both groups the next morning before and after treatment. After serum separation, enzyme-linked immunosorbent assay (ELISA) [ 16 ] was used to measure C-reactive protein (CRP), procalcitonin (PCT), and IL-6 in children following the operation steps of ELISA kits.…”

Section: Methodsmentioning

confidence: 99%

Efficacy of Glucocorticoid plus Intravenous Immunoglobulin in Children with Immunoglobulin-Insensitive Kawasaki Disease

Zhang

Fan

2022

Journal of Healthcare Engineering

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This study mainly analyzes the clinical effect of glucocorticoid (GC) plus intravenous immunoglobulin (IVIG) in treating children with immunoglobulin (Ig)-insensitive Kawasaki disease (KD). From September 2013 to November 2021, 86 Ig-insensitive KD children were selected, including 46 children (observation group, Obs) treated with GC plus IVIG, and 40 children (control group, Con) treated with IVIG. The symptom (fever and fever) resolution time, inflammatory factors (C-reactive protein, CRP; procalcitonin, PCT; interleukin-6, IL-6), immune indicators (CD3+, CD4+, CD8+ T lymphocytes CD3+, CD4+, and CD4+/CD8+), and incidence of adverse reactions were compared between the groups. The results identified shorter fever and rash resolution time in Obs compared with Con. The posttreatment CRP, PCT, IL-6, and CD8+ and the incidence of adverse events reduced notably in Obs and were lower than Con, while CD3+, CD4+, and CD4+/CD8+ elevated statistically and were higher than that of Con. Our results indicate that GC plus IVIG can significantly promote symptom resolution, alleviate inflammatory response, and improve immune function in children with Ig-insensitivity KD, with favorable safety and clinical promotion value.

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Section: Methodsmentioning

confidence: 99%

Efficacy of Glucocorticoid plus Intravenous Immunoglobulin in Children with Immunoglobulin-Insensitive Kawasaki Disease

Zhang

Fan

2022

Journal of Healthcare Engineering

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“…Inflammation indexes: Venous blood from elbow (5 ml) collected 24 h before and after treatment was centrifuged in a 1500 xg centrifuge at 4° for 10 min and the resulting serum was stored in a -70° refrigerator for later use. Enzyme-Linked Immunosorbent Assay (ELISA) [11] detected Interleukin-6 (IL-6), High-Sensitivity C-Reactive Protein (hs-CRP) and Calcitonin (PCT), referring to the instructions of IL-6, hs-CRP and PCT kits (Yipu Biology Co., Ltd., Wuhan, China, YX-E10140, YX-E11183, YX-E10643).…”

Section: Respiratory Mechanics Indexesmentioning

confidence: 99%

Influence of Cefoperazone/Sulbactam plus Azithromycin on Serum Inflammatory Factors and Pulmonary Function in Patients with Severe Lower Respiratory Tract Infection

Zheng¹,

Wang²,

Wan³

2022

ijps

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Influence of Cefoperazone/Sulbactam plus Azithromycin in Severe Lower Respiratory Tract Infection Patients The purpose of this research project was to clarify the influence of Cefoperazone/Sulbactam plus Azithromycin on the alleviation of serum inflammatory factors and the improvement of pulmonary function in patients with severe lower respiratory tract infection, aiming at improving the management of patients with the disease. We first retrospectively analyzed 201 patients with severe lower respiratory tract infection from May 2019 to May 2021, of which 101 patients (research group) were treated with Cefoperazone/ Sulbactam plus Azithromycin and the remaining 100 patients were treated with Cefoperazone/ Sulbactam (control group). The efficacy, incidence of adverse reactions, bacterial clearance rate, recovery (hospitalization time, pulmonary infection control time and fever resolution time), inflammatory factors (interleukin-6, tumor necrosis factor-alpha, thrombocytocrit) and pulmonary function indicators (peak airway pressure, airway resistance, work of breathing, dynamic compliance) were observed and compared. The results revealed notably less time of hospitalization, pulmonary infection control and fever resolution, as well as a statistically higher bacterial clearance rate in research group vs. control group.After treatment, peak airway pressure, airway resistance and work of breathing were lower while dynamic compliance was higher in research group compared with control group; interleukin-6, high-sensitivity C-reactive protein and thrombocytocrit were lower in research group; and the total clinical effective rate was noticeably higher in research group compared with control group (93.07 % vs. 75.00 %). No evident difference was observed in total adverse reactions between the two cohorts. Therefore, this paper argues that Cefoperazone/Sulbactam plus Azithromycin is beneficial to alleviate inflammation and improve the pulmonary function in patients with severe lower respiratory tract infection, which is worth popularizing.

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“…For animals in managed care, such as those in zoological settings, pregnancy determination may guide husbandry practices, ensuring that species-specific needs are provided to support offspring survival. There are several approaches to detecting pregnancy in wildlife species, including ultrasonography [ 1 , 2 , 3 , 4 , 5 ], analysis of urinary or fecal biomarkers such as progesterone [ 6 , 7 ], relaxin [ 8 , 9 ], or 13,14-dihydro-15-keto-PGF2a (PGFM) [ 10 , 11 , 12 ], and detection of hormones found in serum, such as pregnancy associated glycoproteins (PAGs) [ 13 , 14 ]. Nevertheless, the development of a reliable method to diagnose pregnancy has proven elusive in many species, despite this menu of diagnostic tools.…”

Section: Introductionmentioning

confidence: 99%

Label-Free Quantification (LFQ) of Fecal Proteins for Potential Pregnancy Detection in Polar Bears

Curry

Philpott

Wojtusik

et al. 2022

Life

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Reliable pregnancy diagnostics would be beneficial for monitoring polar bear (Ursus maritimus) populations both in situ and ex situ, but currently there is no method of non-invasive pregnancy detection in this species. Recent reports in several carnivore species described the identification of fecal proteins that may serve as pregnancy biomarkers; however, repeatability has been limited. The objective of the current analysis was to utilize an unbiased, antibody-free, label-free method for the identification and quantification of fecal proteins to determine if differences associated with pregnancy are detectable in polar bears. Protein was extracted from fecal samples (n = 48) obtained from parturient (n = 6) and non-parturient (n = 6) profiles each at four timepoints: pre-breeding season, embryonic diapause, early placental pregnancy, and mid-placental pregnancy. Protein was prepared and analyzed on the Thermo Orbitrap Eclipse nanoLC-MS/MS system. A total of 312 proteins was identified and quantified; however, coefficients of variation (CV) were high for both abundance ratio variability (384.8 ± 61.0% SEM) and within group variability (86.8 ± 1.5%). Results of this study suggest that the inconsistencies in specific protein concentrations revealed previously by antibody-based assays may not be due to that methodology’s limitations, but rather, are reflective of true variation that exists among samples.

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Evaluation of a Visual Enzyme-Linked Immunosorbent Assay (Elisa) for Pregnancy Detection in Four Ungulate Species

Cited by 4 publications

References 31 publications

Efficacy of Glucocorticoid plus Intravenous Immunoglobulin in Children with Immunoglobulin-Insensitive Kawasaki Disease

Efficacy of Glucocorticoid plus Intravenous Immunoglobulin in Children with Immunoglobulin-Insensitive Kawasaki Disease

Influence of Cefoperazone/Sulbactam plus Azithromycin on Serum Inflammatory Factors and Pulmonary Function in Patients with Severe Lower Respiratory Tract Infection

Label-Free Quantification (LFQ) of Fecal Proteins for Potential Pregnancy Detection in Polar Bears

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