Evaluation of a supplemental assay for the diagnosis of hepatitis C virus infections

Rihn, Bertrand; Hussenet, F; Detry, Marie-Blanche; Catelle, A.; Faou, Alain Le

doi:10.1016/s1201-9712(00)90065-1

Cited by 4 publications

(2 citation statements)

References 10 publications

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“…The blood was left to clot in incubator for 15 min. then centrifuged and the separated serum was collected in sterile tube; liver function tests including serum ALT and AST, serum ALB (Heidelbaugh and Bruderly., 2006)., Detection of anti-HCV antibody using enzyme linked immunosorbentassay (ELISA) :Antibodies to hepatitis C virus (HCV) were qualitatively determined by using ETI-AB-HCVK-3 (P00626) Sorin Biomedica kit (Sorine Biomedica, Diagnostic Division, 1304, Suluggia, Vercelli, Italy) (Rihn et al, 2000). Pathological processing for liver biopsy specimens was as follows, 4 u sections were prepared from paraffin blocks and stained for hematoxyline and eosin stain (H & E), massontrichrom, reticulin, periodic acid Schiff stain (PAS) and PAS-diastase stains (Bancroft and Stevens., 1975).…”

Section: Blood Samplingmentioning

confidence: 99%

Serum hyaluronic acid as non invasive biomarker to predict liver fibrosis in viral hepatitis patients.

Agroudy

Elghareb

Addissouky

et al. 2016

Journal of Bioscience and Applied Research

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Fibrosis is a hallmark histologic event of viral hepatitis and is characterized by the excessive accumulation and reorganization of the extracellular matrix (ECM). The gold standard for assessment of fibrosis is liver biopsy. As this procedure has various limitations, including risk of patient injury and sampling error. Serum Hyaluronic acid as non invasive marker for liver fibrosis is desirable. The present study aims to determine the serum hyaluronic acid (HA) levels as biochemical marker of hepatic fibrosis and cirrhosis and correlate it with the degree of hepatic fibrosis. Serum HA level in chronic hepatitis patients (n=60) are divided into two groups, group1: included 30 patients positive for anti-HCV (antibodies), group 2 included 30 patients positive for HBsAg, and controls (n=10) were assessed by ELISA and liver histopathological parameters were evaluated by the modified Knodell score and microscopic examination of liver biopsies from patients. Individuals in healthy control group have normal levels of HA (mean 14.3, SD: 5.5) while the levels of HA were elevated in patients of HCV alone (mean 103.6±28.0) and in patient of HCV (mean 104.5± 37.5).Also levels of HA were poorly elevated in HBV alone (mean 62.2± 15.5) and in HBV (mean 45.8± 12.4). showed that serum HA levels are well correlated with HAI in patients of HBV & HCV groups where, there was significant increase in HA levels by increase of HAI by liver biopsy P < 0.001.HA levels and stages of fibrosis were well correlated in patients of HBV and HCV group. Where, this is a significant increase in HA levels when Considering F0 to F6 scores by liver biopsy (P < 0.001). Serum HA is a useful non-invasive marker of liver fibrosis. There is a strong positive correlation between serum HA levels and degree of liver fibrosis. The concentration of serum HA rises according to progression of liver fibrosis.

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Section: Blood Samplingmentioning

confidence: 99%

Serum hyaluronic acid as non invasive biomarker to predict liver fibrosis in viral hepatitis patients.

Agroudy

Elghareb

Addissouky

et al. 2016

Journal of Bioscience and Applied Research

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show abstract

“…A normal ALT level was defined as less than 40 IU/l. Testing for anti-HCV was performed by ELISA using Sorine Biomedica kits [Sorine Biomedica Diagnostics Division, Saluggia (VC), Italy] [10].…”

Section: Laboratory Testingmentioning

confidence: 99%

Alanine aminotransferase levels in citizens of Nile Delta infected with the chronic hepatitis C virus

Abdel-Khalek¹,

Elfakhry²,

Helaly³

et al. 2012

Egyptian Liver Journal

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Borderline and/or discordant Cobas Amplicor™ HCV Test version 2.0 results: clinical significance

Valentine-Thon

2001

Journal of Clinical Virology

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Evaluation of a supplemental assay for the diagnosis of hepatitis C virus infections

Abstract: Despite clear results and good sensitivity, the MATRIX-HCV2 assay was poorly predictive of viremia in patients with indeterminate results in initial screening assays.

Cited by 4 publications

References 10 publications

Serum hyaluronic acid as non invasive biomarker to predict liver fibrosis in viral hepatitis patients.

Serum hyaluronic acid as non invasive biomarker to predict liver fibrosis in viral hepatitis patients.

Alanine aminotransferase levels in citizens of Nile Delta infected with the chronic hepatitis C virus

Borderline and/or discordant Cobas Amplicor™ HCV Test version 2.0 results: clinical significance

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