Evaluation of a solid-phase immunoradiometric assay (IRMA) for serum thyroglobulin: effect of anti-thyroglobulin autoantibody

Mariotti, Stefano; C, Cupini; Giani, Carlotta; Lari, Riccardo; Rolleri, E.; Falco, A.; Marchisio, Marco; Pinchera, Aldo

doi:10.1016/0009-8981(82)90181-4

Cited by 45 publications

(12 citation statements)

References 18 publications

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“…As the assessment of endogenous antibodies simultaneously by several methods is too complicated and expensive, most laboratories employ ordinary agglutination tests. According to our results, both the Tg concentration and the avidity of Tg antibodies affect antigen-antibody binding, as also suggested by Mariotti et al [4].…”

Section: Discussionsupporting

confidence: 90%

Thyroid antibodies in association with thyroid malignancy II: qualitative properties of thyroglobulin antibodies

Haapala¹,

Soppi²,

Mörsky³

et al. 1995

Scandinavian Journal of Clinical and Laboratory Investigation

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Qualitative properties of thyroglobulin (Tg) antibodies, in association with thyroid malignancy, suspected malignancy or other thyroid diseases, were studied in 177 patients. Retrospective clinical analysis revealed 137 patients to have thyroid carcinoma and 40 to have other thyroid diseases. Serum Tg was assayed by an immunoradiometric method. Thyroid microsomal (AMC) and Tg antibodies were measured by the particle agglutination method and the avidity of Tg antibodies by enzyme immunoassay (EIA). Assessment of the qualitative properties of Tg antibodies revealed that the high-avidity antibodies especially seem to bind circulating Tg. Thus any Tg value from a sample with detectable Tg antibodies is unreliable and should be interpreted with caution.

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Section: Discussionsupporting

confidence: 90%

Thyroid antibodies in association with thyroid malignancy II: qualitative properties of thyroglobulin antibodies

Haapala¹,

Soppi²,

Mörsky³

et al. 1995

Scandinavian Journal of Clinical and Laboratory Investigation

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“…This divergence might be explained by differences in endogenous and exogenous Tg, the use of different isoforms with variable immunoreactivity in the preparation of the test (21), and TgAb heterogeneity (22).…”

Section: Discussionmentioning

confidence: 99%

Antithyroglobulin antibodies in patients with differentiated thyroid carcinoma: methods of detection, interference with serum thyroglobulin measurement and clinical significance

Rosário

Maia

Fagundes

et al. 2004

Arq Bras Endocrinol Metab

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Antithyroglobulin antibodies (TgAb) were measured using a chemiluminescent immunoassay (ICMA) and an agglutination test. TgAb laboratory and clinical interference with Tg measurements were assessed. The course of TgAb concentration and disease status were compared during 3 years after initial treatment. The agglutination test failed to detect all titers < 10IU/mL (ICMA). Interference from TgAb was common at high titers, but even low antibody titers (< 5IU/mL) were able to interfere with Tg measurement. Cases of distant metastases with undetectable Tg (by IRMA) and those apparently free of disease and without thyroid remnants with Tg> 2ng/ml (by RIA) were identified among patients with TgAb. The exogenous Tg recovery test was normal (> 80%) by the two methods in 22% of patients with TgAb and confirmed laboratory interference. Absence of reduction in TgAb levels was a marker of persistent disease. In conclusion, TgAb should be determined by immunoassays; interference with Tg measurements occurred mainly but not always at high concentrations, with a normal Tg recovery test not excluding this interference. The behavior of TgAb is related to disease persistence or cure. Anticorpos anti-tireoglobulina (TgAb) foram medidos por um imunoensaio de quimioluminescência (ICMA) e um teste de aglutinação. Avaliamos a interferência clínica e laboratorial dos TgAb com as medidas de Tg. A evolução da concentração dos TgAb e o estado da doença foram comparados durante 3 anos após o início do tratamento. O teste de aglutinação falhou em detectar todos o títulos <10UI/mL (ICMA). Interferência dos TgAb foi comum com títulos altos, mas mesmo títulos baixos dos anticorpos (<5UI/mL) interferiram na medida de Tg. Casos com metástases à distância e Tg indetectável (por IRMA) e aqueles aparentemente livres da doença e sem remanescentes tireoidenos com Tg >2ng/ml (por RIA) foram identificados entre pacientes com TgAb. O teste de recuperação da Tg exógena foi normal (>80%) por ambos os métodos em 22% dos pacientes com TgAb, confirmando a interferência laboratorial. Ausência de redução dos níveis de TgAb foi um marcador de persistência da doença. Em conclusão, TgAb deve ser determinada por imunoensaios; interferência com as medidas de Tg ocorreram principalmente mas não somente em altas concentrações, com um teste normal de recuperação de Tg não excluindo esta interferência. O comportamento dos TgAb está relacionado à persistência ou à cura da doença.

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“…However, interferences invariably lead to falsely decreased values (Mariotti et al 1982) and should be sought by a recovery test in all sera. These were found in 5.5% of patients, which is a lower proportion than when using a RIA with polyclonal antibodies.…”

Section: Discussionmentioning

confidence: 99%

A new immunoradiometric assay (IRMA) system for thyroglobulin measurement in the follow-up of thyroid cancer patients

et al. 1991

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A new commercially available kit for thyroglobulin (Tg) measurement [immunoradiometric assay (IRMA) system based on monoclonal antibodies] was used in 479 patients with thyroid carcinoma. The effective working range was 1 ng/ml, and results were strongly correlated with our homemade radioimmunoassay (RIA). This IRMA method is less susceptible to interferences of auto-antibodies than our RIA. During thyroxine (T4) treatment, the Tg level was undetectable in 98% of patients after total thyroid ablation, in 91% after total thyroidectomy and in 42% after lobectomy only. In this situation, Tg was found in all patients with large metastases and in 88% of those with small metastases. Following T4 withdrawal, Tg was detectable in all patients with neoplastic disease and in 13% of those in complete remission after total thyroid ablation. In conclusion, Tg measured with this IRMA method appears to be a reliable marker of differentiated thyroid carcinoma.

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Evaluation of a solid-phase immunoradiometric assay (IRMA) for serum thyroglobulin: effect of anti-thyroglobulin autoantibody

Cited by 45 publications

References 18 publications

Thyroid antibodies in association with thyroid malignancy II: qualitative properties of thyroglobulin antibodies

Thyroid antibodies in association with thyroid malignancy II: qualitative properties of thyroglobulin antibodies

Antithyroglobulin antibodies in patients with differentiated thyroid carcinoma: methods of detection, interference with serum thyroglobulin measurement and clinical significance

A new immunoradiometric assay (IRMA) system for thyroglobulin measurement in the follow-up of thyroid cancer patients

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