Evaluation of a smartphone-based rapid fluorescent diagnostic system for H9N2 virus in specific-pathogen-free chickens

Yeo, Seon Ju; Cuc, Bui Thi; Sung, Haan Woo; Park, Hyun

doi:10.1007/s00705-016-2922-8

Cited by 14 publications

(17 citation statements)

References 17 publications

(20 reference statements)

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“…Recently, modern smartphones are equipped with a lightweight, high-performance camera/imaging module, becoming the preferred tool for use in many POC applications 39 , 40 .…”

Section: Discussionmentioning

confidence: 99%

Improvement of a rapid diagnostic application of monoclonal antibodies against avian influenza H7 subtype virus using Europium nanoparticles

Yeo

Bao

Seo

et al. 2017

Sci Rep

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The development of a sensitive and rapid diagnostic test is needed for early detection of avian influenza (AI) H7 subtype. In this study, novel monoclonal antibodies (mAbs) against influenza A H7N9 recombinant hemagglutinin (rHA)1 were developed and applied to a Europium nanoparticle–based rapid fluorescent immunochromatographic strip test (FICT) to improve the sensitivity of the rapid diagnostic system. Two antibodies (2F4 and 6D7) exhibited H7 subtype specificity in a dot-FICT assay by optimization of the conjugate and the pH of the lysis buffer. The subtype specificity was confirmed by an immunofluorescence assay and Western blot analysis. The limit of detection of the FICT employing novel mAbs 31 ng/mL for H7N9 rHA1 and 40 hemagglutination units/mL for H7 subtype virus. Sensitivity was improved 25-fold using Europium as confirmed by comparison of colloidal gold-based rapid diagnostic kit using the 2F4 and 6D7 mAbs.

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“…Recently, modern smartphones are equipped with a lightweight, high-performance camera/imaging module, becoming the preferred tool for use in many POC applications 39 , 40 .…”

Section: Discussionmentioning

confidence: 99%

Improvement of a rapid diagnostic application of monoclonal antibodies against avian influenza H7 subtype virus using Europium nanoparticles

Yeo

Bao

Seo

et al. 2017

Sci Rep

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“…A further improvement of this branch of methods involves the use of europium nanoparticles [67], artificially designed peptides specifically binding hemagglutinin [68], and improved fluorescent dyes [69]. Additionally, a smartphone-based immunofluorescent field diagnostic approach was described [70,71] and upgraded to a dual-fluorescent system [72] capable of specifically detecting the highly pathogenic H5 subtype. In this type of assay, a special portable fluorescent strip reader is required to develop the test strips, which are compatible with a smartphone camera.…”

Section: Current Avian Influenza Virus Sampling Analysis and Quantification Methodologymentioning

confidence: 99%

Avian Influenza in Wild Birds and Poultry: Dissemination Pathways, Monitoring Methods, and Virus Ecology

et al. 2021

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Avian influenza is one of the largest known threats to domestic poultry. Influenza outbreaks on poultry farms typically lead to the complete slaughter of the entire domestic bird population, causing severe economic losses worldwide. Moreover, there are highly pathogenic avian influenza (HPAI) strains that are able to infect the swine or human population in addition to their primary avian host and, as such, have the potential of being a global zoonotic and pandemic threat. Migratory birds, especially waterfowl, are a natural reservoir of the avian influenza virus; they carry and exchange different virus strains along their migration routes, leading to antigenic drift and antigenic shift, which results in the emergence of novel HPAI viruses. This requires monitoring over time and in different locations to allow for the upkeep of relevant knowledge on avian influenza virus evolution and the prevention of novel epizootic and epidemic outbreaks. In this review, we assess the role of migratory birds in the spread and introduction of influenza strains on a global level, based on recent data. Our analysis sheds light on the details of viral dissemination linked to avian migration, the viral exchange between migratory waterfowl and domestic poultry, virus ecology in general, and viral evolution as a process tightly linked to bird migration. We also provide insight into methods used to detect and quantify avian influenza in the wild. This review may be beneficial for the influenza research community and may pave the way to novel strategies of avian influenza and HPAI zoonosis outbreak monitoring and prevention.

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“…The invention of user-friendly techniques brought measurements to smartphone systems. The study of Yeo et al [76] showed the performance of a smartphone-based rapid fluorescent diagnostic system (SRFDS) created for the H9N2 virus diagnosis in chickens. The authors used oropharyngeal (OP) and cloacal (CL) samples and compared their method with real-time RT-PCR.…”

Section: Novel Detection Methods Of Influenza Virusmentioning

confidence: 99%

Detection Methods of Human and Animal Influenza Virus—Current Trends

Dziąbowska¹,

Czaczyk²,

Nidzworski³

2018

Biosensors

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The basic affairs connected to the influenza virus were reviewed in the article, highlighting the newest trends in its diagnostic methods. Awareness of the threat of influenza arises from its ability to spread and cause a pandemic. The undiagnosed and untreated viral infection can have a fatal effect on humans. Thus, the early detection seems pivotal for an accurate treatment, when vaccines and other contemporary prevention methods are not faultless. Public health is being attacked with influenza containing new genes from a genetic assortment between animals and humankind. Unfortunately, the population does not have immunity for mutant genes and is attacked in every viral outbreak season. For these reasons, fast and accurate devices are in high demand. As currently used methods like Rapid Influenza Diagnostic Tests lack specificity, time and cost-savings, new methods are being developed. In the article, various novel detection methods, such as electrical and optical were compared. Different viral elements used as detection targets and analysis parameters, such as sensitivity and specificity, were presented and discussed.

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Evaluation of a smartphone-based rapid fluorescent diagnostic system for H9N2 virus in specific-pathogen-free chickens

Cited by 14 publications

References 17 publications

Improvement of a rapid diagnostic application of monoclonal antibodies against avian influenza H7 subtype virus using Europium nanoparticles

Improvement of a rapid diagnostic application of monoclonal antibodies against avian influenza H7 subtype virus using Europium nanoparticles

Avian Influenza in Wild Birds and Poultry: Dissemination Pathways, Monitoring Methods, and Virus Ecology

Detection Methods of Human and Animal Influenza Virus—Current Trends

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