Evaluation of a simple plasma catecholamine extraction procedure prior to high-performance liquid chromatography and electrochemical detection

Ganhao, M.F.; Hattingh, J.; Hurwitz, Marilyn L.; Pitts, Neville

doi:10.1016/0378-4347(91)80069-o

Cited by 54 publications

(26 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The main difference between the two methods was the elution solution for the extraction of CA from the alumina. By using a mixture of methanol and acetic acid (98:2, v/v, pH 2.8) in place of 0.1 M perchloric acid (pH 1.2) by Ganhao et al [9]. as the elution solution, the recovery and reproducibility of plasma CA analysis was improved.…”

Section: Discussionmentioning

confidence: 99%

“…The extraction procedure used in this study was a modification of the method described by Ganhao et al [9]. The main difference between the two methods was the elution solution for the extraction of CA from the alumina.…”

Section: Discussionmentioning

confidence: 99%

“…The extraction procedure reported by Ganhao et al [9] was modified. An aliquot (1.0 ml) of the plasma sample was transferred into a sepakol minicolumn with a column filter and a bottom cap (Shiseido).…”

Section: Methodsmentioning

confidence: 99%

“…In the modified method a mixture of acetic acid and methanol (98:2, v/ v, pH 2.8) was used in place of the 0.1 M perchloric acid (pH 1.2) in the Ganhao et al method [9]. In a chromatogram of plasma extracts obtained by the procedure of Ganhao et al [9], the peaks of many substances that are endogenous to equine plasma were detected and disturbed the detection of CA (Fig. 1b).…”

Section: Effect Of the Modified Extraction Proceduresmentioning

confidence: 99%

“…There have been many papers published on the analytical methods used for detecting plasma CA, such as the radioenzymatic method [2,22], high-performance liquid chromatography (HPLC) with electrochemical detection (ED) [4,9], and fluorimetric detection [18,21].…”

mentioning

confidence: 99%

See 4 more Smart Citations

Circadian Variations in Plasma Adrenaline and Noradrenaline in the Thoroughbred Horse.

Kurosawa

Takeda

Nagata

et al. 1997

JES

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Effect Of the Modified Extraction Proceduresmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Circadian Variations in Plasma Adrenaline and Noradrenaline in the Thoroughbred Horse.

Kurosawa

Takeda

Nagata

et al. 1997

JES

View full text Add to dashboard Cite

show abstract

Epinephrine for the treatment of anaphylaxis: do all 40 mg sublingual epinephrine tablet formulations with similar in vitro characteristics have the same bioavailability?

Rawas‐Qalaji

Simons

2006

Biopharm & Drug Disp

View full text Add to dashboard Cite

Epinephrine autoinjectors are underutilized in the first aid emergency treatment of anaphylaxis in the community; so non-invasive sublingual epinephrine administration is being proposed. In order to determine the effect of changing excipients on the bioavailability of sublingual epinephrine, four distinct fast-disintegrating epinephrine 40 mg tablet formulations, A, B, C and D, were manufactured using direct compression. All formulations were evaluated for tablet hardness (H), disintegration time (DT) and wetting time (WT). In a prospective 5-way crossover study, four sublingual formulations and epinephrine 0.3 mg i.m. as a control were tested sequentially in a validated rabbit model. Blood samples were collected before dosing and at intervals afterwards. Epinephrine plasma concentrations were measured using HPLC-EC. All tablet formulations met USP standards for weight variation and content uniformity, and resulted in similar mean H, DT and WT (n=6). The area under the curve (AUC), maximum concentration (C(max)) and time at which C(max) was achieved (T(max)) did not differ significantly after the sublingual administration of formulation A and epinephrine 0.3 mg i.m. The AUC after B, C and D were significantly lower (p<0.05) than after epinephrine 0.3 mg i.m. These results suggest that the selection of excipients used in these tablet formulations can affect the bioavailability of sublingually administered epinephrine.

show abstract

Improved Survival of Embryonic Porcine Dopaminergic Neurons in Coculture with a Conditionally Immortalized GDNF-Producing Hippocampal Cell Line

Meyer¹,

Johansen

Gramsbergen³

et al. 2000

Experimental Neurology

View full text Add to dashboard Cite

Evaluation of a simple plasma catecholamine extraction procedure prior to high-performance liquid chromatography and electrochemical detection

Cited by 54 publications

References 16 publications

Circadian Variations in Plasma Adrenaline and Noradrenaline in the Thoroughbred Horse.

Circadian Variations in Plasma Adrenaline and Noradrenaline in the Thoroughbred Horse.

Epinephrine for the treatment of anaphylaxis: do all 40 mg sublingual epinephrine tablet formulations with similar in vitro characteristics have the same bioavailability?

Improved Survival of Embryonic Porcine Dopaminergic Neurons in Coculture with a Conditionally Immortalized GDNF-Producing Hippocampal Cell Line

Contact Info

Product

Resources

About