Evaluation of a routine hematology analyzer for quality control of leukoreduced plasma

Abstract

BACKGROUND: Quality control of residual white blood cells (WBCs) and red blood cells (RBCs) in leukoreduced plasma is mandatory. Although technological advances have been made, analysis of quality controls using routine hematology analyzers has not generally been introduced. The aim of this study was to evaluate if the routine hematology analyzer Sysmex XN-10, (Sysmex Nordic ApS) could be used for quality control of residual WBCs and RBCs in leukoreduced plasma. ABBREVIATIONS: CV = coefficient of variation; rRBCs = residualRBCs; rWBCs = residual WBCs.From the

Residual red cells in blood components: A multisite study of fully automated enumeration using a hematology analyzer

“…Early studies, however, suggested that manual sample processing steps could inhibit introduction into routine automated QM processes 13 . A study that used the BF mode on the Sysmex XN‐10 analyzer concluded that it was suitable for rRBC counting in plasma at a level of 6000 RBCs/μL, but manual mixing was required and performance with PCs was not assessed 15 …”

“…The Sysmex XN series of analysers (Sysmex Corp., Kobe, Japan) also includes an option for low‐level cell counting in BFs that has been reported as giving comparable results with microscopy, although limitations were noted with levels of RBCs in BFs less than 1000 cells/μL 14 . This analysis mode has also been described as being suitable for rRBC enumeration in leukoreduced plasma, with satisfactory performance at 6000 RBCs/μL, although it was unsuitable for counting residual white blood cells (rWBCs) 15 …”