Evaluation of a recombinant antigen-based antibody assay for diagnosis of bancroftian filariasis in Egypt

Ramzy, Reda M. R.; Helmy, Hanan; Faris, Rifky; Gad, Ahmed; Chandrashekar, Ramaswamy; Weil, Gary J.

doi:10.1080/00034983.1995.11812974

Cited by 35 publications

(38 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…4 Prevalence rates of IgG4 antibodies to the recombinant filarial antigen BmM14 were higher than rates for microfilaremia or antigenemia. Prior studies have shown that this test is quite specific for exposure or infection by filarial parasites, 6,13 and Wuchereria bancrofti is the only filarial parasite of humans known to be endemic in Egypt.…”

Section: Discussionmentioning

confidence: 99%

“…8 IgG4 antibodies to recombinant filarial antigen BmM14 were detected by ELISA as previously described. 6,8 Prior studies have shown that most (90%) subjects with microfilaremia or antigenemia have positive antibody test results; positive antibody test results are also seen in some apparently uninfected endemic normals. Prior studies have shown that the filarial antigen test and the BmM14 antibody test have specificities that approach 100% with nonendemic Egyptian sera.…”

Section: Methodsmentioning

confidence: 99%

“…5 Assays for IgG4 antibody to recombinant filarial antigens can also be useful tools for measuring exposure to filarial parasites in populations. 6 Relatively little is known about protective immunity in human filariasis, and there is little direct evidence that such immunity exists. The present study was prompted by the observation that filariasis prevalence rates increase rapidly with age in teenage children in Egypt, 5 and by results of another study that found that filariasis infection intensity in a village in the Nile Delta tended to decrease with age 7 (also Weil GJ and others, unpublished data).…”

mentioning

confidence: 99%

See 2 more Smart Citations

A longitudinal study of Bancroftian filariasis in the Nile Delta of Egypt: baseline data and one-year follow-up.

Weil¹,

Ramzy²,

Setouhy³

et al. 1999

The American Journal of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Abstract. We initiated a longitudinal study of Bancroftian filariasis to improve understanding of dynamics and risk factors for infection in villages near Cairo, Egypt. Baseline prevalence rates for microfilaremia and filarial antigenemia for 1,853 subjects more than 9 years of age were 7.7% and 11.2%, respectively. Microfilaria counts, antigen levels, and microfilaremia incidence over a 1-year period were all significantly lower in older people. These findings suggest that humans develop partial immunity to Wuchereria bancrofti over time. One-year incidence rates for microfilaremia and antigenemia were 1.8% and 3.1%, respectively. Filarial antigenemia, IgG4 antibody to recombinant antigen BmM14, and household infection were all significant risk factors for microfilaremia incidence. Microfilaria counts and parasite antigen levels were significantly reduced by diethylcarbamazine therapy, but many infected subjects refused treatment, and most treated people were still infected one year later. Incident infections approximately balanced infections lost to produce an apparent state of dynamic equilibrium.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

A longitudinal study of Bancroftian filariasis in the Nile Delta of Egypt: baseline data and one-year follow-up.

Weil¹,

Ramzy²,

Setouhy³

et al. 1999

The American Journal of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

show abstract

“…17,22,23 Previous studies have shown that the antigen-capture ELISA and the card test used in this study have comparable sensitivities, with positive tests in 90-97% of sera or plasma from untreated MF carriers. 15,24 Filariasis treatment reduces adult worm infection intensities and antigen levels. Thus, prior treatment of many infected subjects in the study area (and unusually low filarial antigen levels in the study population) may explain the relatively low sensitivity of antigen testing in microfilaremic subjects observed in this study.…”

Section: Discussionmentioning

confidence: 99%

The impact of single-dose diethylcarbamazine treatment of bancroftian filariasis in a low-endemicity setting in Egypt.

Ramzy¹,

El-Setouhy²,

Helmy³

et al. 2002

The American Journal of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Abstract. This study was designed to evaluate the effect of a single dose of diethylcarbamazine (DEC, 6 mg/kg) on Wuchereria bancrofti infections in a low-endemicity setting in Egypt (microfilaremia, or MF, 3.7%, median MF 34/mL). Subjects with MF or filarial antigenemia were treated and restudied 1 year later. Treatment with DEC dramatically reduced blood MF counts, with clearance in 69% of subjects. Treatment also reduced filarial antigen levels, but low clearance rates suggest that some adult worms survived treatment in most patients. Mass treatment was administered in one village; 27 months later, MF prevalence had decreased 84% (from 4.9% to 0.8%). These results show that singledose DEC treatment can have a major effect on MF prevalence rates and levels in low-endemicity settings. Although the World Health Organization advocates repeated multidrug regimens for filariasis elimination, mass treatment with DEC alone may be sufficient to interrupt transmission in areas with low infection intensities and prevalence rates.

show abstract

“…KAP -Knowledge, attitudes and practices. Weil & Liftis, 19871997Freedman et al, 1997Ramzy et al, 1999Bhumiratana et al, 1999 To meet this objective, a large-scale transmission control requires a current magnitude and geographical distribution of the disease in the at-risk population. To understand the extent to which the target population needs to be designed for MDA and monitored whether the MDA implementation is effective, such surveillance and monitoring systems are required.…”

Section: Surveillance and Monitoring Systemsmentioning

confidence: 99%

Molecular Diagnosis and Monitoring of Benzimidazole Susceptibility of Human Filariids

Bhumiratana¹,

Intarapuk²,

Sangthong³

et al. 2012

Current Topics in Tropical Medicine

View full text Add to dashboard Cite

Evaluation of a recombinant antigen-based antibody assay for diagnosis of bancroftian filariasis in Egypt

Cited by 35 publications

References 8 publications

A longitudinal study of Bancroftian filariasis in the Nile Delta of Egypt: baseline data and one-year follow-up.

A longitudinal study of Bancroftian filariasis in the Nile Delta of Egypt: baseline data and one-year follow-up.

The impact of single-dose diethylcarbamazine treatment of bancroftian filariasis in a low-endemicity setting in Egypt.

Molecular Diagnosis and Monitoring of Benzimidazole Susceptibility of Human Filariids

Contact Info

Product

Resources

About