Evaluation of a Real-time PCR-based assay using the lightcycler system for detection of Toxoplasma gondii bradyzoite genes in blood specimens from patients with toxoplasmic retinochoroiditis

Molecular Detection and Genotyping of Toxoplasma gondii from Clinical Samples

“…To increase the sensitivity of molecular diagnostics of toxoplasmosis nested PCR was introduced, although in recent years real-time PCR has shown a significantly higher sensitivity as well as specificity (Jauregui et al, 2001;Reischl et al, 2003;Contini et al, 2005;Calderaro et al, 2006;Edvinsson et al, 2006). Real-time PCR detection also has the capability of quantification of T. gondii in biological samples, which has found wide application in monitoring the kinetics and outcome of infection in patients undergoing therapy, as well as in experimental models (Lin et al, 2000;Jauregui et al, 2001;Contini et al, 2005;Djurković-Djaković et al, 2012).…”

“…Molecular methods based on polymerase chain reaction (PCR) are simple, sensitive, reproducible and can be applied to all clinical samples (Bell and Ranford-Cartwright, 2002;Contini et al, 2005;Calderaro et al, 2006;Bastien et al, 2007). These methods are divided into two groups.…”

A quantitative real-time PCR assay for Ehrlichia ruminantium using pCS20

“…DNA-based tests including a pCS20 based PCR and PCR/ 32 P-probe ( [Waghela et al, 1991], [Steyn et al, 2003] and [Van Heerden et al, 2004b]) have been used to detect E. ruminantium both in ticks and ruminants. The PCR/probe-based assays are labour-intensive, time consuming (5 days) and requires handling of radioactive materials ( [Contini et al, 2005] and [Monis et al, 2002]) while real-time PCR assays can detect parasite DNA within 70 min, are more sensitive and less labour-intensive (Espy et al, 2006). E. ruminantium, quantitative real-time PCR was first used to detect the map1 gene in order to monitor cell culture vaccine production (Peixoto et al, 2005).…”

Thyroid-Stimulating Hormone Regulation and Transcription in Hypothyroidism

“…It combines a thermocycler and a microvolume fluorimeter (Lyon, 2001) with the fluorescencebased assay requiring less manipulation than a basic PCR assay (Contini et al, 2005). Therefore, the LightCycler® is a highly sensitive quantitative method for the detection of RNA expression (Emrich et al, 2002;Schuster et al, 2004;Tan et al, 2004).…”