Evaluation of a rapid test for the detection of antibodies to human immunodeficiency virus type 1 and 2

Ng, Kee Peng; Saw, T.L.; Baki, Ali; He, Jin-Shu; Singh, N. P.; Lyles, Cynthia M.

doi:10.1177/095646249901000608

Cited by 4 publications

(3 citation statements)

References 3 publications

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“…Our results demonstrate that rapid assays can have high sensitivity and specificity and, when used in combination with serial or parallel testing algorithms, can yield results with an accuracy comparable to that of results obtained by ELISAbased testing algorithms. The high sensitivity and specificity we observed in this study confirm and extend findings of others (1,4,6,8 Our findings of a high sensitivity and high specificity for some rapid EIAs, especially for samples from seroconverters of HIV-1 non-B subtypes, and the fact that reagents do not need to be reconstituted for some of the assays have important public health utility in Africa. First, by using rapid EIA testing algorithms, we have observed a remarkable increase in the number of women identified as HIV positive who were eligible .…”

Section: Discussionsupporting

confidence: 78%

Sensitivity and Specificity of Human Immunodeficiency Virus Rapid Serologic Assays and Testing Algorithms in an Antenatal Clinic in Abidjan, Ivory Coast

et al. 2001

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To evaluate serologic testing algorithms for human immunodeficiency virus (HIV) based on a combination of rapid assays among persons with HIV-1 (non-B subtypes) infection, HIV-2 infection, and HIV-1-HIV-2 dual infections in Abidjan, Ivory Coast, a total of 1,216 sera with known HIV serologic status were used to evaluate the sensitivity and specificity of four rapid assays: Determine HIV-1/2, Capillus HIV-1/HIV-2, HIV-SPOT, and Genie II HIV-1/HIV-2. Two serum panels obtained from patients recently infected with HIV-1 subtypes B and non-B were also included. Based on sensitivity and specificity, three of the four rapid assays were evaluated prospectively in parallel (serum samples tested by two simultaneous rapid assays) and serial (serum samples tested by two consecutive rapid assays) testing algorithms. All assays were 100% sensitive, and specificities ranged from 99.4 to 100%. In the prospective evaluation, both the parallel and serial algorithms were 100% sensitive and specific. Our results suggest that rapid assays have high sensitivity and specificity and, when used in parallel or serial testing algorithms, yield results similar to those of enzyme-linked immunosorbent assay-based testing strategies. HIV serodiagnosis based on rapid assays may be a valuable alternative in implementing HIV prevention and surveillance programs in areas where sophisticated laboratories are difficult to establish.

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Section: Discussionsupporting

confidence: 78%

Sensitivity and Specificity of Human Immunodeficiency Virus Rapid Serologic Assays and Testing Algorithms in an Antenatal Clinic in Abidjan, Ivory Coast

et al. 2001

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“…Throughout the last decade, these assays have demonstrated excellent sensitivities and specificities, but many tests still require a laboratory to process the specimens and to maintain the integrity of the test components (5,10,19,20,26). The World Health Organization (WHO) has recommended the use of rapid HIV tests for blood safety, for surveillance, and for patient diagnosis in prenatal clinics or voluntary counseling and testing (VCT) centers (1,3).…”

mentioning

confidence: 99%

Evaluation of Rapid Prenatal Human Immunodeficiency Virus Testing in Rural Cameroon

Granade

Parekh

Tih

et al. 2005

Clin Vaccine Immunol

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Pregnant women (n ‫؍‬ 859) in rural Cameroonian prenatal clinics were screened by two rapid human immunodeficiency virus (HIV) antibody tests (rapid tests [RT]) (Determine and Hema-Strip) using either whole blood or plasma. One additional RT (Capillus, HIV-CHEK, or Sero-Card) was used to resolve discordant results. RT results were compared with HIV-1 enzyme immunoassay (EIA) and Western blot (WB) results of matched dried blood spots (DBS) to assess the accuracy of HIV RTs. DBS EIA/WB identified 83 HIV antibody-reactive, 763 HIV antibody-nonreactive, and 13 indeterminate specimens. RT results were evaluated in serial (two consecutive tests) or parallel (two simultaneous tests) testing algorithms. A serial algorithm using Determine and Hema-Strip yielded sensitivity and specificity results of 97.6% and 99.7%, respectively, whereas a parallel RT algorithm using Determine plus a second RT produced a sensitivity and specificity of 100% and 99.7%, respectively. HIV RTs provide excellent alternatives for identifying HIV infection, and their field performance could be monitored using DBS testing strategies.

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“…Its specificity is ≥ 98.9% and requires supplementary serological testing to confirm diagnosis [29][30][31]. These tests are extremely useful in situations requiring fast detection of infection, such as following occupational exposure.…”

Section: Acquired Immunodeficiency Syndrome (Aids)mentioning

confidence: 99%

Sexually-transmitted viral diseases in women: clinical and epidemiological aspects and advances in laboratory diagnosis

Pinto¹,

Baggio²,

Guedes³

2005

Braz J Infect Dis

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Sexually transmitted diseases (STDs) have long been known, but they have only recently been recognized as causes of significant long-term morbidity, mainly as a result of increased knowledge concerning viral STDs. The relationship of these diseases with conditions such as anogenital cancer and acquired immunodeficiency syndrome (AIDS) has made viral STDs an important issue in the healthcare of women and infants, and in reproductive health. The evolution of the AIDS pandemic is now characterized by growing differences between rich and poor nations. New diagnostic tools include rapid tests of blood, urine and saliva samples. New techniques, such as computerized cytology, have been developed for the diagnosis of human papillomavirus (HPV). Women infected with HIV are at a greater risk of being co-infected with HPV, and they are also more prone to the progression and persistence of HPV lesions. The herpes simplex virus presents high rates of co-infection with HIV, and it plays a particularly important role in increasing transmission rates of this virus.

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Evaluation of a rapid test for the detection of antibodies to human immunodeficiency virus type 1 and 2

Cited by 4 publications

References 3 publications

Sensitivity and Specificity of Human Immunodeficiency Virus Rapid Serologic Assays and Testing Algorithms in an Antenatal Clinic in Abidjan, Ivory Coast

Sensitivity and Specificity of Human Immunodeficiency Virus Rapid Serologic Assays and Testing Algorithms in an Antenatal Clinic in Abidjan, Ivory Coast

Evaluation of Rapid Prenatal Human Immunodeficiency Virus Testing in Rural Cameroon

Sexually-transmitted viral diseases in women: clinical and epidemiological aspects and advances in laboratory diagnosis

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