Evaluation of a Rapid Immunochromatographic Test for Detection of Brucella Abortus Antibodies in Egyptian Cattle Sera and Milk

Elshemey, Tharwat M.; Abdelrahman, Amir

doi:10.5455/ajvs.47307

Cited by 10 publications

(8 citation statements)

References 9 publications

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“…Hence, in the present investigation, LFA was evaluated with RBPT and iELISA considering cELISA as the gold standard. Our results are in agreement with the high sensitivity and specificity observed for the flow assay by earlier reports from Spain [ 27 ], Turkey [ 28 , 29 ] Kazakhstan [ 28 ], and Egypt [ 30 , 31 ]. The LFA has shown good PPV and NPV greater than RBPT and almost similar to that of iELISA.…”

Section: Discussionsupporting

confidence: 93%

Evaluation of lateral flow assay as a field test for investigation of brucellosis outbreak in an organized buffalo farm: A pilot study

Shome¹,

Filia²,

Padmashree³

et al. 2015

Vet World

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Aim:The aim was to evaluate lateral flow assay (LFA) as a field test for investigation of brucellosis outbreak in organized buffalo farm.Materials and Methods:A total of 153 serum samples were tested to detect the presence of brucella antibodies by LFA and three other serological tests i.e. rose bengal plate test (RBPT), protein G based indirect enzyme-linked immunoassay (iELISA), and competitive ELISA (cELISA). The performances of LFA and other serological tests were evaluated using OIE complaint cELISA as the gold standard.Results:Serological tests revealed 50% of the animals were seropositive for Brucella antibodies and correlated with clinical history of abortions, infertility, and productive failures. The newly developed assay showed 87.1% and 92.6% sensitivity and specificity, which was even higher than the specificity of RBPT.Conclusions:The investigation proved the potential usefulness of LFA for field diagnosis of brucellosis in the regions where laboratory facilities are limited.

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Section: Discussionsupporting

confidence: 93%

Evaluation of lateral flow assay as a field test for investigation of brucellosis outbreak in an organized buffalo farm: A pilot study

Shome¹,

Filia²,

Padmashree³

et al. 2015

Vet World

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“…In general, there was a moderate degree of agreement between all the three tests in both human and animal with kappa value of 0.57 in animals and 0.51 in humans 95% confidence interval and p<0.001 which was statistically significant. This was similar to the findings of Elshemey and Abd-Elrahman [16].…”

Section: Discussionsupporting

confidence: 92%

Comparison of rose bengal plate test, serum agglutination test, and indirect enzyme-linked immunosorbent assay in brucellosis detection for human and goat samples

Ipola¹,

Kato²,

Ikwap³

et al. 2018

Int J One Health

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Background: In Uganda, detection of brucellosis in goats and humans is more frequently done using rose bengal plate test (RBPT) in comparison to other serological tests such as serum agglutination test (SAT) and indirect enzyme-linked immunosorbent assay (i-ELISA). Aim:This study aimed at estimating the degree of agreement, specificity and sensitivity of RBPT, SAT, and ELISA in brucellosis detection in human and goat samples.Materials and Methods: An analytical study was conducted involving parallel testing of 425 goat samples and 177 human samples to compare RBPT, SAT, and i-ELISA tests of brucellosis detection. Results:A seroprevalence of 14.82% in animals and 11.86% in humans was detected using RBPT, 7.06% in goats and 5.09% in humans using SAT, and 6.35% in humans and 4.52% in animals using i-ELISA. The degree of agreement between the three tests in humans and animals was compared using Fleiss' kappa statistics where a moderate agreement (MA) was obtained for both with kappa value 0.57 in animals and 0.51 in humans and p<0.001. Kappa values in animals were 0.52 (MA) for RBPT and i-ELISA, 0.61 (MA) for RBPT and SAT, and 0.82 substantial agreement (SA) for SAT and i-ELISA. The kappa values in humans were 0.52 (MA) for RBPT and i-ELISA, 0.57 (MA) for RBPT and SAT, and 0.82 (SA) for SAT and i-ELISA, respectively. The study revealed a significant agreement in the tests for all the three tests, but there was SA between SAT and i-ELISA in both animal and human samples. Sensitivity and specificity of RBT and SAT were estimated using i-ELISA as a gold standard as follows, in goats RBPT: 100 and 91% and SAT: 100 and 99%, respectively, and in humans RBPT: 100 and 92% and SAT: 88 and 99%, respectively. Conclusion:It appears that RBPT is good as a screening test, whereas SAT and i-ELISA are good for diagnosis purposes or confirmatory test.

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“…As well as this test has several practical advantages making it the method of choice when testing animals from nomadic and other migratory populations [14]. Relatively similar findings were recorded by Elshemey and Abd-Elrahman [15] who recorded 94.44 % sensitivity and 100 % specificity suggesting that, it is a simple and rapid method that provides accurate detection of antibodies to B. abortus in cattle. Therefore, it can be practically used in the epidemiological surveillances of brucellosis.…”

Section: Resultssupporting

confidence: 61%

Studies on ruminant brucellosis in El Salam canal area, Egypt

Wassif

Mohamed

2017

Benha Journal of Applied Sciences

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The current study is aimed to provide some epidemiological data about brucellosis and its impact among ruminants in the area around El-Salam canal, North Sinai. In addition to determination of the sensitivity, specificity; positive & negative predictive values and diagnostic efficacy of Brucella Antibody Test Kits as a rapid field assay for the diagnosis of brucellosis. Our results revealed that, the overall prevalence rate of brucellosis was 7.1% (12/168) and 10.1% (17/168) using ELISA and Brucella Antibody Test Kits respectively. Out of 168 serum samples; 9 were seropositive and 148 were sero-negative for brucellosis by both ELISA and Brucella Antibody Test Kits. Another 3 and 8 serum samples were only positive with ELISA and Brucella Antibody Test Kits respectively, revealing 75% sensitivity, 94.8% specificity, 47% positive predictive value, 53.5% negative predictive value and 93.4% diagnostic efficacy for this test. Brucellosis was found to be one of the causes of abortion in the study area as 61.9% (13/21) of aborted animals were seropositive for brucellosis. While to a little extent, it can be considered one of infertility causes as 4.7% (7/147) of infertile animals were seropositive for brucellosis. On the other hand, there were 38% (8/21) and 95.2% (140/147) of aborted and infertile animals respectively were sero-negative for brucellosis revealing that, there were another causes of abortion and infertility in the area of study rather than brucellosis and there was a necessity for their further investigations in order to improve the fertility and productivity of the animals in this targeted area.

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Evaluation of a Rapid Immunochromatographic Test for Detection of Brucella Abortus Antibodies in Egyptian Cattle Sera and Milk

Cited by 10 publications

References 9 publications

Evaluation of lateral flow assay as a field test for investigation of brucellosis outbreak in an organized buffalo farm: A pilot study

Evaluation of lateral flow assay as a field test for investigation of brucellosis outbreak in an organized buffalo farm: A pilot study

Comparison of rose bengal plate test, serum agglutination test, and indirect enzyme-linked immunosorbent assay in brucellosis detection for human and goat samples

Studies on ruminant brucellosis in El Salam canal area, Egypt

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