Evaluation of a rapid coagglutination (COA) test for the detection of infectious pancreatic necrosis virus (IPNV) in tissue samples of Atlantic salmon, Salmo salar L.

Taksdal, T; Thorud, K.

doi:10.1046/j.1365-2761.1999.00154.x

Cited by 15 publications

(9 citation statements)

References 19 publications

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“…Mortalities were monitored daily for about 5 weeks. An IPNV rapid agglutination kit was used on head kidney samples from dead fish to verify IPN as the cause of death (58). Mortality results from replicates with no statistically significant differences were pooled.…”

Section: Methodsmentioning

confidence: 99%

CpG DNA Induces Protective Antiviral Immune Responses in Atlantic Salmon (Salmo salarL.)

Jørgensen¹,

Johansen²,

Steiro³

et al. 2003

J Virol

121

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Section: Methodsmentioning

confidence: 99%

CpG DNA Induces Protective Antiviral Immune Responses in Atlantic Salmon (Salmo salarL.)

Jørgensen¹,

Johansen²,

Steiro³

et al. 2003

J Virol

121

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“…Co-agglutination has also been used for detection of infectious pancreatic necrosis virus in the tissue sample of Atlantic salmon [9]. The most important use of the co-agglutination test in the diagnosis of fish disease would be to detect the specific viral antigen in the tissue of infected fish and it was found that viral antigen could be detected in extract of whole organ or affected tissue [3].…”

Section: Resultsmentioning

confidence: 99%

“…The co-agglutination test method that used in the research is the serology method test which needs less time and less cost. This method proved to have an advantage with simple characteristic, fast, accurate and cheap, especially for fields application [9]. But, in Indonesia, this technique has not gotten attention and sufficient development.…”

mentioning

confidence: 99%

Development of Co-agglutination Method for Viral Nervous Necrosis from Grouper (Epinephelus sp.) in Batam

Amanu¹,

Rifai²,

Syarief³

et al. 2015

JAST-B

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Viral nervous necrosis (VNN) has been reported to infect larvae and juvenile of humpback grouper, and until now, this virus is one of main problem for humpback grouper. Co-agglutination test proved to be a simple, rapid and reliable diagnostic test suitable for use in the field or laboratory without any special apparatus. The study aimed to study application of a co-agglutination test using Staphylococci sensitized with specific antibody for the diagnostic of VNN in grouper. First, brain and eye organ samples from diseased fish are homogenized with phosphate buffer saline (PBS) of pH 7.2. Then, the supernatant is collected after centrifugation at 8,000 rpm for 20 min, and finally one drop of the supernatant and one drop of anti VNN antibody sensitized Staphylococci suspension are mixed on a glass slid and observation of the agglutination is performed after 5-10 min. The result shows that co-agglutination technique detects positive VNN in the brain and eye samples. The co-agglutination technique may provide valid result in a very short time as compared with complex method, such as enzyme-linked immunosorbant essay (ELISA) and fluorescient antibody technique (FAT) that require a high cost. Thus, this test can detect VNN faster, simple and more economic.

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“…In both the trial series, mortality per group was recorded daily throughout the immunization and challenge periods. Onset of the IPN epizootic was confirmed by a IPNV co‐agglutination test (Taksdal & Thorud 1999). In the second round of trials, kidney samples from all dead fish were frozen and a selection was subjected to confirmatory diagnosis using IPNV Ag ELISA (Testline Ltd).…”

Section: Methodsmentioning

confidence: 99%

Strong genetic influence on IPN vaccination‐and‐challenge trials in Atlantic salmon, Salmo salar L.

Ramstad

Midtlyng

2008

Journal of Fish Diseases

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Two series of experimental challenge trials were performed for evaluation of multivalent oil-adjuvanted vaccines with and without an infectious pancreatic necrosis virus (IPNV) antigen component. In both the trial series, Atlantic salmon were hatched, reared, vaccinated and subjected to temperature and light manipulation to induce smoltification. When ready for sea the fish were transported to the VESO Vikan experimental laboratory for bath or cohabitant challenge with IPNV. In the first series, four vaccination and bath challenge trials involving 2-year classes of experimental fish were conducted. In the second series, three groups of eyed eggs of Atlantic salmon allegedly differing in their innate resistance to IPNV were used (Storset, Strand, Wetten, Kjøglum & Ramstad 2007). Hatching, rearing and smoltification were synchronized for each group, and fish from each genetic group were randomly allocated IPN vaccine, reference vaccine or saline before being placed into parallel tanks for bath or cohabitant challenge. In the first series of trials, IPN-specific mortality commenced on day 10-12 after bath challenge. Replicates showed similar results. In trials 1 and 2 belonging to the same experimental fish year class, the average cumulative control mortality reached 60.6% and 79.5%, respectively, whereas in trials 3 and 4 belonging to the following year class the control mortality was consistently below 50%. In the second series of trials, the experimental fish originating from allegedly IPN susceptible parents consistently showed the highest cumulative mortality among the unvaccinated controls (>75%) whereas smolts derived from allegedly IPNV resistant parents showed only 26-35% control mortality. The IPN-vaccinated fish experienced significantly improved survival vs. the fish immunized with reference vaccine, with RPS values above 75% in the IPN susceptible strain. In the IPN resistant strain, the protection outcomes were variable and in part non-significant. The outcome of both the trial series suggests that control mortalities above 50% are necessary to reliably demonstrate specific protection with IPN vaccines.

show abstract

Evaluation of a rapid coagglutination (COA) test for the detection of infectious pancreatic necrosis virus (IPNV) in tissue samples of Atlantic salmon, Salmo salar L.

Cited by 15 publications

References 19 publications

CpG DNA Induces Protective Antiviral Immune Responses in Atlantic Salmon (Salmo salarL.)

CpG DNA Induces Protective Antiviral Immune Responses in Atlantic Salmon (Salmo salarL.)

Development of Co-agglutination Method for Viral Nervous Necrosis from Grouper (Epinephelus sp.) in Batam

Strong genetic influence on IPN vaccination‐and‐challenge trials in Atlantic salmon, Salmo salar L.

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