Evaluation of a quantitative fluorescence immunoassay (FIAX) for detection of serum antibody to Borrelia burgdorferi

Pennell, D R; Wand, Philip J.; Schell, Ronald F.

doi:10.1128/jcm.25.11.2218-2220.1987

Cited by 17 publications

(9 citation statements)

References 14 publications

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“…Thus, the total anti-B. burgdorferi antibody response detected in infected hamsters was similar to those detected in other infected animals (1,14) and humans (12,15,19,24,38,41). The borreliacidal antibody response, however, decreased with the elimination of spirochetes from the host.…”

Section: Discussionsupporting

confidence: 79%

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Detection of anti-Borrelia burgdorferi antibody responses with the borreliacidal antibody test, indirect fluorescent-antibody assay performed by flow cytometry, and western immunoblotting

Creson

Lim

Glowacki

et al. 1996

Clin Diagn Lab Immunol

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Borreliacidal antibodies participate in the resolution of Lyme disease by clearing Borrelia burgdorferi sensu lato from the host. Detection of borreliacidal antibodies is also valuable for determination of the specific serodiagnosis of Lyme disease. We show in this work that antibody detected by the borreliacidal antibody test did not correlate with antibody detected by the indirect fluorescent-antibody assay or Western immunoblotting. Detection of borreliacidal antibody decreased with elimination of the spirochete from the host in the presence or absence of therapy. By contrast, the antibody responses detected by the indirect fluorescent-antibody assay or Western immunoblotting remained elevated or continued to expand, respectively. This suggests that the borreliacidal antibody test is a prognostic indicator for clearance of the spirochete. Additional investigations with humans are needed to confirm the prognostic potential of the borreliacidal antibody test.

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Section: Discussionsupporting

confidence: 79%

“…The most common test methods used for the serodiagnosis of Lyme disease, however, are the indirect fluorescent-antibody assay, various formats of the enzyme immunoassay, and Western immunoblotting (12,24,38,41). These assays detect the total serum antibody response directed against B. burgdorferi sensu lato, including natural and cross-reactive antibodies.…”

mentioning

confidence: 99%

Detection of anti-Borrelia burgdorferi antibody responses with the borreliacidal antibody test, indirect fluorescent-antibody assay performed by flow cytometry, and western immunoblotting

Creson

Lim

Glowacki

et al. 1996

Clin Diagn Lab Immunol

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“…Furthermore, the titer values of each serum tested scored by the 12 laboratories were within one twofold dilution. These results, as well as the results by Pennell et al (15), show the efficacy of FIA-L as an antibody test for Lyme disease.…”

Section: Discussionsupporting

confidence: 79%

Fluoroimmunoassay studies with solubilized antigens from Borrelia burgdorferi

et al. 1989

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Sodium deoxycholate-solubilized Borrelia burgdorferi antigen was prepared for use in a solid-phase fluoroimmunoassay (FIA-L) to detect antibodies in Lyme disease. Serum specimens were tested by FIA-L and by a microimmunofluorescence test. The FIA-L results are comparable to those of the standard microimmunofluorescence test. The overall agreement was 0.98. Moreover, the FIA-L procedure is simple and rapid; fluorescence is objectively determined and is proportional to antibody titer.

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“…We drew blood from three healthy human volunteers who had no history of Lyme disease and who had not taken antibiotics for the 30 days before phlebotomy. Lyme serologies, done by the fluorescent immunoassay (FIAX) method, 24 were negative for all subjects. One person was group A, Rh positive, and two persons were group 0, Rh positive on standard blood typing technique.…”

Section: Methodsmentioning

confidence: 99%

Survival of Borrelia burgdorferi in human blood stored under blood banking conditions

Nadelman¹,

Sherer

Mack

et al. 1990

Transfusion

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Hematogenous dissemination of organisms occurs in many spirochetal diseases, including Lyme disease and syphilis. Although syphilis has been transmitted by transfusion, in the vast majority of cases, only fresh blood products were involved, in part because Treponema pallidum survives poorly when refrigerated in citrated blood. Because of the rising incidence of Lyme disease in certain areas, whether its causative agent, Borrelia burgdorferi, could survive under blood banking conditions was studied. Dilutions of stock cultures of two strains of B. burgdorferi were inoculated into samples of citrated red cells (RBCs). Viable spirochetes were recovered from RBCs inoculated with 10(6) organisms per mL, after refrigeration for as long as 6 weeks. It is concluded that B. burgdorferi may survive storage under blood banking conditions and that transfusion-related Lyme disease is theoretically possible.

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Evaluation of a quantitative fluorescence immunoassay (FIAX) for detection of serum antibody to Borrelia burgdorferi

Cited by 17 publications

References 14 publications

Detection of anti-Borrelia burgdorferi antibody responses with the borreliacidal antibody test, indirect fluorescent-antibody assay performed by flow cytometry, and western immunoblotting

Detection of anti-Borrelia burgdorferi antibody responses with the borreliacidal antibody test, indirect fluorescent-antibody assay performed by flow cytometry, and western immunoblotting

Fluoroimmunoassay studies with solubilized antigens from Borrelia burgdorferi

Survival of Borrelia burgdorferi in human blood stored under blood banking conditions

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