Evaluation of a platelet lysate bilayered system for periodontal regeneration in a rat intrabony three‐wall periodontal defect

Babo, Pedro S.; Cai, Xinjie; Plachokova, Adelina S.; Reis, Rui L.; Jansen, John A.; Gomes, Manuela E.; Walboomers, X. Frank

doi:10.1002/term.2535

Cited by 16 publications

(29 citation statements)

References 45 publications

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“…This study demonstrated that the bilayer sponges combined with cell and biological factors promoted significantly higher osteochondral regeneration and reduced the defect size, as observed by radiography, quantitative computed tomography and macroscopic and histological evaluation 4 months after surgery, than the control group (unloaded bilayer sponges). In a similar approach, a bilayered system has been explored for the regeneration of periodontium in a critical 3wall intrabony periodontal defect in rats [188]. (nano/meso scale control) and freeze-drying (fibrillar structures) to produce 3D…”

Section: Controlled Architecturementioning

confidence: 99%

Blood derivatives awaken in regenerative medicine strategies to modulate wound healing

Mendes

Gomez‐Florit

Babo

et al. 2018

Advanced Drug Delivery Reviews

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Blood components play key roles in the modulation of the wound healing process and, together with the provisional fibrin matrix ability to selectively bind bioactive molecules and control its spatial-temporal presentation, define the complex microenvironment that characterize this biological process. As a biomimetic approach, the use of blood derivatives in regenerative strategies has awakened as a source of multiple therapeutic biomolecules. Nevertheless, and despite their clinical relevance, blood derivatives have been showing inconsistent therapeutic results due to several factors, including proper control over their delivery mechanisms. Herein, we highlight recent trends on the use biomaterials to protect, sequester and deliver these pools of biomolecules in tissue engineering and regenerative medicine approaches. Particular emphasis is given to strategies that enable to control their spatiotemporal delivery and improve the selectivity of presentation profiles of the biomolecules derived from blood derivatives rich in platelets. Finally, we discussed possible directions for biomaterials design to potentiate the aimed regenerative effects of blood derivatives and achieve efficient therapies.

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Section: Controlled Architecturementioning

confidence: 99%

Blood derivatives awaken in regenerative medicine strategies to modulate wound healing

Mendes

Gomez‐Florit

Babo

et al. 2018

Advanced Drug Delivery Reviews

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“…In 70. showed that the stabilization of platelet-origin proteins on the root surface increased the overall periodontal healing score for a period up to 6 weeks after implantation and restricted the formation of long epithelial junctions [14]. The recent findings of Noguchi S. et al (2017) suggest that the destruction of periodontal tissue is increased in tissue containing long junctional epithelium compared with normal junctional epithelium, and that the immunized condition accelerates the destruction by forming immune complexes [22].…”

mentioning

confidence: 97%

“…Nevertheless, according to Alves A. et al (2019) reports, there is a lack of evidence data from animal models, which would be useful for studies of new periodontal treatments [12]. Scaling and root planing may not always be effective in preventing periodontal disease progression, and, moreover, with currently available therapies, full regeneration of lost periodontal tissues after periodontitis cannot be achieved [13,14].…”

mentioning

confidence: 99%

“…Material and methods. The rat models, used in the reviewed publications were: ligature-induced [5,13,[15][16][17][18][19], streptozotocin-induced [2,3,20,21] and immune complexes-induced [22] experimental periodontitis; 3-wall periodontal defect in Wistar rats [9,14],osteoporotic rats periodontal fenestration defect model [4], apical periodontitis, induced in Wistar rats by pulp exposure to oral cavity [23], altered functional loading model with use of a hard and soft diet and the posterior bite-blocks in rats [24], as well as rats stress exposures in different modes [25], experimental rat model of surgically created chronic acid reflux esophagitis [26]. The combined bilayered compartmentalized system was used to promote the regeneration of alveolar bone in Wistar rats [14].…”

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confidence: 99%

“…Nevertheless, the faster degradation of the cement component with incorporated hyaluronic acid microspheres compromised the stability of the combined compartmentalized system, which hampered the periodontal regeneration. Therefore, after optimization of the hard tissue analogue, the system should be further elaborated in (pre)clinical validation studies to assist predictable healing of functional periodontium [14]. Micro-computed tomography and histological analysis showed substantially more new bone formation in the platelet-rich fibrin + cells group at 24 days after surgery.…”

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confidence: 99%

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Periodontal Destruction and Regeneration in Experimental Models: Combined Research Approaches

Kordiyak¹

2020

Ukr. ž. med. bìol. sportu

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Chronic periodontitis is a common dental disease, resulting in destruction of gingival tissue, periodontal ligament, cementum, alveolar bone and, consequently- teeth loss in the adult population. Experimental animal models have enabled the study of periodontal disease pathogenesis and are used to test new therapeutic approaches for treating the disease The purpose of this review study was to draw the evidence from animal models, required for future assessment of destructional and regenerative processes in periodontal tissues. Material and methods: a rat experimental periodontitis models of ligature, streptozotocin, and immune complexes induced periodontitis, periodontal defect, altered functional loading, stress exposures and surgically created chronic acid reflux esophagitis models. Histomorphomorphological/-metrical, immunohisto (-cyto)chemical and histopathological analysis, micro-computed tomography, scanning and transmission electron microscopy, polarizing light and confocal microscopy, spectrophotometry, radiographic and biomechanical analysis, descriptive histology and computer-assisted image analysis. Results and discussion. Scaling and root planing may not always be effective in preventing periodontal disease progression, and, moreover, with currently available therapies, full regeneration of lost periodontal tissues after periodontitis cannot be achieved. However, in 70.5% of the results of experimental studies reported, irrespective of the defect type and animal model used, beneficial outcome for periodontal regeneration after periodontal ligament stem cell implantation, including new bone, new cementum and new connective tissue formation, was recorded. Therefore, platelet-rich fibrin combined with rat periodontal ligament stem cells provides a useful instrument for periodontal tissue engineering. Conclusion. There is sufficient evidence from preclinical animal studies suggesting that periodontal tissue engineering would provide a valuable tool for periodontal regeneration. Further elaboration of the developed in preclinical studies experimental techniques should justify progress to clinical studies and subsequent medical application

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Tissue Engineered Constructs for Periodontal Regeneration: Current Status and Future Perspectives

Vaquette

Pilipchuk

Bartold

et al. 2018

Adv Healthcare Materials

108

109

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The periodontium, consisting of gingiva, periodontal ligament, cementum, and alveolar bone, is a hierarchically organized tissue whose primary role is to provide physical and mechanical support to the teeth. Severe cases of periodontitis, an inflammatory condition initiated by an oral bacterial biofilm, can lead to significant destruction of soft and hard tissues of the periodontium and result in compromised dental function and aesthetics. Although current treatment approaches can limit the progression of the disease by controlling the inflammatory aspect, complete periodontal regeneration cannot be predictably achieved. Various tissue engineering approaches are investigated for their ability to control the critical temporo-spatial wound healing events that are essential for achieving periodontal regeneration. This paper reviews recent progress in the field of periodontal tissue engineering with an emphasis on advanced 3D multiphasic tissue engineering constructs (TECs) and provides a critical analysis of their regenerative potential and limitations. The review also elaborates on the future of periodontal tissue engineering, including scaffold customization for individual periodontal defects, TEC's functionalization strategies for imparting enhanced bioactivity, periodontal ligament fiber guidance, and the utilization of chair-side regenerative solutions that can facilitate clinical translation.

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Evaluation of a platelet lysate bilayered system for periodontal regeneration in a rat intrabony three‐wall periodontal defect

Cited by 16 publications

References 45 publications

Blood derivatives awaken in regenerative medicine strategies to modulate wound healing

Blood derivatives awaken in regenerative medicine strategies to modulate wound healing

Periodontal Destruction and Regeneration in Experimental Models: Combined Research Approaches

Tissue Engineered Constructs for Periodontal Regeneration: Current Status and Future Perspectives

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