Evaluation of a particle-enhanced turbidimetric immunoassay for the measurement of ferritin: application to patients participating in an autologous blood transfusion program

Gómez, Frederic; Simó, Josep M.; Camps, Jordi; Clivillé, X; Bertran, Núria; Ferré, Natàlia; Bofill, C; Joven, Jorge

doi:10.1016/s0009-9120(00)00064-3

Cited by 21 publications

(21 citation statements)

References 18 publications

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“…The analysis of serum ferritin was assessed by immunoassay as described by Gomez et al [11]. Serum iron and serum transferrin were measured using spectrophotometry (ITC Diagnostics S.A., Barcelona, Spain and Biokit S.A., Barcelona, Spain respectively) with a Beckman Coulter analyzer (Fullerton, California, USA) at the Centro de Investigación Biomédica (CRB of the Hospital Sant Joan de Reus).…”

Section: Methodsmentioning

confidence: 99%

Diet, iron biomarkers and oxidative stress in a representative sample of Mediterranean population

Romeu

Aranda

Giralt

et al. 2013

Nutr J

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BackgroundThe consumption pattern characterized by high consumption of vegetables, fruit, fish, olive oil and red wine has been associated with improvements in the total antioxidant capacity of individuals and reduced incidence of diseases related to oxidation. Also, high body iron levels may contribute to increase the oxidative stress by the generation of reactive oxygen species. The objective of this study is to analyze the relationship between antioxidant and pro-oxidant factors obtained from the diet and iron biomarkers on lipoprotein oxidation and total antioxidant capacity in a representative sample of the Mediterranean population.MethodsCross-sectional prospective study, carried out with 815 randomly selected subjects (425 women and 390 men). Dietary assessment (3-day food records), iron biomarkers (serum ferritin, serum iron and transferrin saturation), biochemical markers of lipoperoxidation (TBARS), antioxidant capacity (ORAC) and CRP (C-Reactive Protein) were determined. Multiple Linear Regression (MLR) models were applied to analyze the association between diet factors and iron biomarkers on TBARS and ORAC levels.ResultsWe observed that lipoperoxidation measured by TBARS increased by age but no differences were observed by sex. Antioxidant capacity measured by ORAC is independent of age and sex. In general, increasing age, tobacco, heme iron intake from meat and fish and transferrin saturation were independently and positively associated with TBARS, while non-heme iron was negatively associated. Vegetables, vitamin C intake and serum ferritin were positively associated with ORAC, whereas saturated fatty acids and meat intake were negatively associated.ConclusionsIn our general population, we observed that oxidative stress is related to aging, but antioxidant capacity is not. The highest intake of dietary non-heme iron, vegetables and vitamin C intake exerts a protective effect against oxidation while the highest intake of dietary heme iron from meat and fish and saturated fatty acids are associated with increased oxidative stress. High levels of circulating iron measured by transferrin saturation are associated with increased oxidative stress in women however its association with the higher levels of serum ferritin is controversial.

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Section: Methodsmentioning

confidence: 99%

Diet, iron biomarkers and oxidative stress in a representative sample of Mediterranean population

Romeu

Aranda

Giralt

et al. 2013

Nutr J

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“…Serum ferritin (SF; μg/L) was measured immuno-turbidimetrically [25]. The transferrin saturation index was calculated as reported [26].…”

Section: Methodsmentioning

confidence: 99%

Effects of C282Y, H63D, and S65C HFE gene mutations, diet, and life-style factors on iron status in a general Mediterranean population from Tarragona, Spain

et al. 2010

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Mutations in the HFE gene result in iron overload and can produce hereditary hemochromatosis (HH), a disorder of iron metabolism characterized by increased intestinal iron absorption. Dietary quality, alcoholism and other life-style factors can increase the risk of iron overload, especially among genetically at risk populations. Polymorphisms of the HFE gene (C282Y, H63D and S65C) were measured together with serum ferritin (SF), transferrin saturation (TS) and hemoglobin, to measure iron status, in randomly-selected healthy subjects living in the Spanish Mediterranean coast (n = 815; 425 females, 390 males), 18 to 75 years of age. The intake of dietary components that affect iron absorption was calculated from 3-day dietary records. The presence of C282Y/H63D compound heterozygote that had a prevalence of 2.8% in males and 1.2% in females was associated with an elevated TS and SF. No subject was homozygous for C282Y or S65C. The C282Y heterozygote, H63D heterozygote and homozygote and H63D/S65C compound heterozygote genotypes were associated with increased TS relative to the wild type in the general population. These genotypes together with the alcohol and iron intake increase the indicators of iron status, while calcium intake decreases them. We did not observe any affect of the S65C heterozygote genotype on these levels. All the HFE genotypes except for the S65C heterozygote together with the alcohol, iron and calcium intake affect the indicators of iron status. The C282Y/H63D compound heterozygote genotype has the higher phenotypic expression in our Spanish Mediterranean population.

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“…Serum ferritin levels were measured by turbidimetric immunoassay, as described by Gomez et al [30]. They were aliquoted and stored at À80°C until analysis.…”

Section: Biochemical Variablesmentioning

confidence: 99%

Increased iron levels and lipid peroxidation in a Mediterranean population of Spain

Aranda

Fernández‐Cao

Tous

et al. 2016

Eur J Clin Investigation

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Evaluation of a particle-enhanced turbidimetric immunoassay for the measurement of ferritin: application to patients participating in an autologous blood transfusion program

Cited by 21 publications

References 18 publications

Diet, iron biomarkers and oxidative stress in a representative sample of Mediterranean population

Diet, iron biomarkers and oxidative stress in a representative sample of Mediterranean population

Effects of C282Y, H63D, and S65C HFE gene mutations, diet, and life-style factors on iron status in a general Mediterranean population from Tarragona, Spain

Increased iron levels and lipid peroxidation in a Mediterranean population of Spain

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