Evaluation of a Novel Polihexanide-Preserved Wound Covering Gel on Dermal Wound Healing

Abstract

Objective: Daily wound assessment, including dressing changes and the removal of old ointments causes discomfort for the patient. We therefore developed a new thermoreversible and transparent gel formulation that allows for filling wounds of different shapes and depths. The aim of the study was to investigate the effect of a wound covering gel on wound healing and the skin’s microcirculation. Materials and Methods: Investigations were carried out in a standardized and reproducible wound model (hairless mice SKH1/hr, n = 30). Three groups were studied by intravital fluorescence microscopy: treatment with polihexanide-preserved wound covering gel, a formulation containing 3% povidone (PVP)-iodine, and physiological saline for control. Microcirculatory standard parameters were analysed. Results: The non-perfused area vanished within 14 days due to angiogenesis. The venular diameter, oedema formation and functional capillary density showed no significant differences between the three groups. Conclusion: The use of the newly developed wound covering gel has no toxic effects on microcirculation and angiogenesis and reveals no significant differences in the overall assessment of microcirculation compared to the control group and the well-established PVP-iodine. The transparent antibacterial wound covering gel allows for direct wound assessment. Due to its thermoreversible gel formulation it enables good wound contact and easy handling.

Evaluation of angiogenesis, epithelialisation and microcirculation after application of polyhexanide, chitosan and sodium chloride in rodents

“…Its transparency allows a direct assessment of the wound. Our previous studies as well as the data obtained from other groups showed excellent results concerning toxicity and wound healing in experimental and clinical setups .…”

“…The purpose of this study was to examine the effects of polyhexanide ointment and chitosan solution on angiogenesis, epithelialisation and microcirculation in a standardised mouse model on fresh wounds . The wound model in hairless mice allows for qualitative and quantitative analysis of the wound microcirculation after topical application of different formulations and wound dressings .…”

“…Chitosan is a polysaccharide of randomly distributed β‐ ‐linked d ‐glucosamine. It is a natural material obtained from crustacean shells.…”

Clinical Safety and Efficacy of a Novel Thermoreversible Polyhexanide-Preserved Wound Covering Gel

“…Experimental data of the impact of wound gel on microcirculation and wound healing in mice in our research team showed no toxic effects [7] . Another study of our team found an integrity loss of the endothelium and a decrease in the functional vessel density after 1 hour of topical exposures in ears of mice [8] .…”

Classification of Wounds at Risk and Their Antimicrobial Treatment with Polihexanide: A Practice-Oriented Expert Recommendation

“…Flushing of deep wounds (e.g. bite injuries) with topically active strong protein-binding antiseptics is permissible only if drainage of the rinsing fluid is provided [11,33,[41][42][43][44][45] .…”