Evaluation of a novel immunochromatographic device for rapid and accurate clinical detection of Porphyromonas gingivalis in subgingival plaque

Clinical Usefulness of Novel Immunochromatographic Detection Device for Porphyromonas gingivalis in Evaluating Effects of Scaling and Root Planing and Local Antimicrobial Therapy

“…These results reaffirm that DK13‐PG‐001 could identify clinically relevant levels of Pg , and the device score demonstrates a high correlation with number of Pg by the PCR‐invader method. In a previous study, not only patients with periodontitis but also healthy individuals were included to evaluate the device for detection of Pg in subgingival plaque: a positive correlation between DK13‐PG‐001 and PCR‐invader ( r = 0.743) was presented 22 . In this study, the correlation coefficient ( r = 0.862) between DK13‐PG‐001 and PCR‐invader was higher than in the previous study.…”

“…They were immediately transferred into a sampling tube supplied in the test device kit. It was confirmed that contamination of sampling paper points with blood had no significant influence on detection results 22 …”

“…For the detection of Pg in a sample, a novel lateral immunochromatographic device with monoclonal antibodies to Pg (DK13‐PG‐001) was used 22 . Detailed information on the test device kit is described elsewhere 22 …”

“…After 15 minutes at room temperature, detection results were recorded according to appearance of lines in the result window. If results were positive, the intensity of blue coloring was compared with a shade guide, and the corresponding score was given for estimation of bacteria numbers (see http://www.joponline.org/doi/suppl/10.1902/jop.2016.160147/suppl_file/160147_supfig1.html in online Journal of Periodontology ) 22 …”

“…In a recent multicenter clinical study in Japan, it was suggested that the combination of Pg ratio in saliva and serum immunoglobulin G (IgG) titers against Pg may be associated with progression of periodontitis 21 . Imamura et al 22 previously reported on a novel immunochromatographic device (DK13‐PG‐001) for rapid and accurate clinical detection of Pg in subgingival plaque. The device can specifically react to the 40 kDa outer membrane protein of Pg , 23 and a significantly positive correlation was found among the score of DK13‐PG‐001, number of Pg detected by the PCR‐invader method, and periodontal parameters 22 …”