Evaluation of a novel <i>Bacillus</i> strain from a north‐western Spain hot spring as a source of extracellular thermostable lipase

Deive, Francisco J.; Sanromán, M.A.; Longo, María A.

doi:10.1002/jctb.2211

Cited by 12 publications

(10 citation statements)

References 39 publications

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“…Since 1967, when Thomas D. Brock of the University of Wisconsin reported for the first time the existence of thermophilic microorganisms in the hot springs of Yellowstone National Park [5], continental and submarine volcanic areas, geothermally heated sea sediments, and hydrothermal vents all over the world have been described as sources of thermophiles [6][7][8][9][10]. As industrial conditions are usually harsh, thermophilic microorganisms have been gaining wide industrial and biotechnological interest due to the fact that their enzymes are better suited for severe environments.…”

Section: Introductionmentioning

confidence: 99%

A process for extracellular thermostable lipase production by a novel Bacillus thermoamylovorans strain

Deive

Álvarez

Morán

et al. 2012

Bioprocess Biosyst Eng

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A lipolytic enzyme-producing thermophilic microorganism, recently isolated from a hot spring in Galicia (North Western Spain), has been investigated. First, the strain was genetically identified and tentatively named Bacillus thermoamylovorans CH6B. It produced significant levels (around 450 U/L) of extracellular lipolytic activity in shake flask cultures, and the most suitable conditions for this biological process were found at temperatures between 50 and 55 °C, and an initial pH value around 7.0. Next, a preliminary scaling up of the process was carried out in a 5-L stirred tank bioreactor, and it was concluded that operation at agitation and aeration rates of 300 rpm and 0.33 vvm, respectively, were advisable. In both type of cultures, the results were successfully fitted to logistic equations, and the relationship between lipase production and cell growth was investigated. Furthermore, some relevant properties of the crude lipolytic enzyme extracts were assessed. The crude biocatalyst preferentially hydrolysed p-nitrophenyl esters of medium and long-chain fatty acids. Thermal stability in aqueous solution of the produced enzyme was also promising, and the deactivation profiles were fitted to a series-type deactivation model.

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Section: Introductionmentioning

confidence: 99%

A process for extracellular thermostable lipase production by a novel Bacillus thermoamylovorans strain

Deive

Álvarez

Morán

et al. 2012

Bioprocess Biosyst Eng

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“…Factors such as agitation-aeration, pH and temperature, as well as culture medium composition, exert a relevant influence on the production of lipases. Previous studies on lipolytic enzymes production by other thermophilic strains indicated that the process was strongly affected by cultivation variables, such as the mineral content of the culture medium and the degree of aeration amongst others [11][12][13][14]. However, the effects of operating variables on the production of microbial metabolites are strongly strain dependent, and it remains to be ascertained if T. aquaticus YT1 might show a similar behaviour to other Thermus strains.…”

Section: Introductionmentioning

confidence: 89%

“…For instance, Deive et al [14], Kumar et al [16] and Fuciños et al [15] reported an optimum pH at 8.0, 8.5 and 9.0 for lipolytic enzymes from Bacillus subtilis (RRL 1789), Bacillus coagulans BTS-3 and Thermus thermophilus HB27, respectively, using p-nitrophenyl esters to quantify lipolytic activity. In relation to the optimum temperature, in most of the literature on thermoactive lipases, the values range from 50 to 80°C, as described in the case of the lipases from Alcaligenes sp., Bacillus sp.…”

Section: Preliminary Characterization Of the Produced Lipolytic Enzymementioning

confidence: 98%

Scaling-up the production of thermostable lipolytic enzymes from Thermus aquaticus YT1

López

Domínguez

Deive

et al. 2012

Bioprocess Biosyst Eng

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The lipolytic enzymes synthesized by Thermusaquaticus YT1 present extremely interesting properties of thermostability (more than 70% of activity after 12 days at 80°C and a half-life time of 1 h at 95°C), which point out the interest of proposing efficient strategies to successfully tackle the scale-up of the production process. In this study,viable scaling-up of the production process was implemented,and relevant aspects affecting the enzyme synthesis, such as the mineral composition of the culture medium, the aeration and the agitation have been evaluated.A strategy combining the modification of the culture medium and the aeration degree was also approached by adding perfluorocarbons, compounds which improve the availability of oxygen in the culture medium. An opposite response of biomass and lipolytic activity to the aeration conditions was found between scales (about 600 U L(-1) at high aeration levels in flask vs. 150 U L(-1) at high aeration rates in reactor), which further demonstrates the important role of the hydrodynamic conditions on the suitable development of the biological process. In all cases, the cultures were kinetically characterized and the Luedeking and Piret model turned out to be a valuable tool to conclude that the produced lipolytic enzyme is a growth-associated metabolite, no matter the medium and the scale.

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“…Although there is a variable effect depending on the microorganism and the lipidic substances, in general terms, fatty acids, triglycerides, and some esters have been reported to act as good inducers of lipolytic enzymes [4,34,35]. In this case, oleic acid, tributyrin, several oils (from olive, sunflower, and corn), and surfactants (Tween 80, Triton X-100, and CHAPS) were used to check their possible role as lipase inducers.…”

Section: Addition Of Lipidic Compoundsmentioning

confidence: 99%

“…During the last months, several reports were published stressing the need of searching for operating conditions in bioreactors to produce lipolytic enzymes from yeasts [9,35], bacteria [10], and fungi [21], but maximum activities of 700 U/L, 400 U/L, and 500 U/L, respectively, were obtained via the p-nitrophenyl esters method. Therefore, the extremely high levels obtained in this work with the same enzyme determination method point out the suitability of the proposed strategy as well as the bioreactor configuration to be implemented at higher scale for the production of lipolytic enzymes.…”

Section: Bioreactor Culturementioning

confidence: 99%

Culture Conditions and Investigation of Bioreactor Configurations for Lipase Production by Rhizopus oryzae

et al. 2010

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Lipolytic enzymes are the subject of great industrial and academic interest. For this reason, a detailed study of lipolytic enzyme production by the fungus Rhizopus oryzae is tackled, and several steps from plate to shake flasks and bioreactor cultures are investigated in order to propose an optimized strategy to perform the biological process. The suitability of several lipidic compounds and surfactants is assessed. Triton X-100 (5 g/L) gives the highest activities with a maximum value of 6320 U/L which is 10-fold the value attained in cultures without addition of lipidic compounds. As there are almost no studies on bench-scale bioreactors, two bioreactor configurations, stirred tank and air-lift, are investigated to determine the most suitable one to carry out the biological reaction. It is demonstrated that the lipolytic activity is strongly enhanced when a stirred-tank bioreactor is used with a maximum value of 3521 U/L within two days which is clearly higher than the values produced by other recently reported species.

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Evaluation of a novel Bacillus strain from a north‐western Spain hot spring as a source of extracellular thermostable lipase

Cited by 12 publications

References 39 publications

A process for extracellular thermostable lipase production by a novel Bacillus thermoamylovorans strain

A process for extracellular thermostable lipase production by a novel Bacillus thermoamylovorans strain

Scaling-up the production of thermostable lipolytic enzymes from Thermus aquaticus YT1

Culture Conditions and Investigation of Bioreactor Configurations for Lipase Production by Rhizopus oryzae

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